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1.
目的分析羊栖菜多糖对S180荷瘤小鼠红细胞相关生化功能影响的研究。方法建立肿瘤动物模型,分高、中、低剂量腹腔给予羊栖菜多糖7 d,采集红细胞,制备红细胞悬液,运用激光共聚焦扫描技术测定荷瘤小鼠红细胞内[Ca2+]的变化;运用相关试剂盒测定红细胞膜表面唾液酸含量和Na+,K+-ATPase及Ca2+,Mg2+-ATPase活性的变化;用流式细胞仪分析红细胞膜电位水平的变化;采用高效毛细管电泳法测定红细胞电泳合淌度的变化。结果羊栖菜多糖能降低荷瘤小鼠红细胞内[Ca2+],升高膜表面唾液酸的含量,增强膜表面Na+,K+-ATPase及Ca2+,Mg2+-ATPase的活性,提高红细胞膜电位水平,提高红细胞的电泳合淌度。结论羊栖菜多糖可调节或恢复S180荷瘤小鼠红细胞多种生理生化功能。 相似文献
2.
Conventional PCR-SSP, which is based on an agarose gel-based read-out, has the disadvantages of time-consuming post-PCR steps and low potential for automation. The aim of our study was to sort out these drawbacks by establishing a fluorescence-based PCR-SSP system for HLA-C. The assay relies on the sequence-specific identification of amplicons with individually labeled probes that are cleaved during successful PCR by the 5'-3' exonuclease activity of the Taq-DNA Polymerase. The oligonucleotides are labeled with a unique and spectrally resolvable fluorescent reporter dye at the 5' terminus (FAM or TET) and a common quencher dye at the 3' terminus (TAMRA). In case of amplification, the reporter escapes from the quenching control caused by the physical separation of the dyes, resulting in a significant increase of the reporter fluorescence. This allows simultaneous and differential detection of the specific HLA (FAM) and internal control (TET) product. The HLA-C fluorotyping information is based on the individual reporter fluorescence released by 18 PCR primer mixes. Using this method, we analyzed 145 samples previously typed with conventional PCR-SSP and found a concordance rate of 100%. Furthermore, fluorotyping revealed quantitative results that may indicate the presence of homozygosity by high signal intensities. This provided extra protection not to miss new alleles which are not amplified by the current primer mixes. These features as well as the capability of high sample throughput and the possibility of automation makes fluorotyping an attractive tool for PCR-based HLA typing. 相似文献
3.
将3′,5′-环胞苷二棕榈酸酯包封于脂质体中.药效学研究表明,药物的脂质体剂型对腹水型肝癌细胞的抑制率比游离药物以及阿糖胞苷脂质体的抑制率高1倍以上.化学动力学实验显示,在pH为6.9时,脂质体中药物的分解反应速度比游离药物慢得多,其有效期比游离药物长3倍以上.用荧光法研究药物分子在脂双层上的状态,探讨了药物包封于脂质体中抗癌活性及化学稳定性提高的原因. 相似文献
4.
YE Qifa 《华中科技大学学报(医学英德文版)》1996,(4)
OxygenationofLiverTransplantsasaPredictorofSuccessYEQifa(DepartmentofSurgery,TongjiHospital,TongjiMedicalUniversity,Wuhan4300... 相似文献
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6.
应用电镜酶细胞化学方法对12例胃癌细胞三磷酸腺苷酶(Mg~(2+)—ATPase,Ca~(2+)—ATPase),5~1—核苷酸酶(5NPase)及胞嘧啶单核苷酸酶(CMPase)进行了超微结构水平的定位观察。结果表明:胃管状腺癌细胞腔缘微绒毛丰富,Mg~(2+)—ATPase、Ca~(2+)—ATPase及5NPase定位于微绒毛表面,反应明显,提示其功能代谢具有极性分布特点。粘液腺癌细胞出、入胞现象活跃,Mg~(2+)—ATPase、Ca~(2+)—ATPase及CMPase反应明显,认为与其粘液的大量分泌,营养物质的摄取与加工以及逃避免疫细胞的杀伤机制有关。低分化腺癌上述酶大都无反应或反应微弱。作者认为胃癌细胞的酶活性及其分布与其分化程度及功能状况有关。 相似文献
7.
The present study was undertaken to evaluate the effect of fenoterol, a selective beta 2-adrenergic agonist, on basophil histamine release. Fenoterol at 10(-7) to 10(-3) M did not inhibit the release of histamine induced by Dermatophagoides farinae extract (D.f.) from leukocytes from allergic patients sensitive to mite. Similarly, there was no suppression of histamine release induced by anti-IgE and formyl-methionyl-leucyl-phenylalanine under the influence of fenoterol. Fenoterol caused a slight inhibition of the calcium ionophore A23187-induced histamine release at 10(-3) M with % inhibition of 11.8 +/- 2.4 (means +/- SEM, P less than 0.05). There was no synergism between fenoterol and theophylline in inhibiting D.f.-induced histamine release. Fenoterol did not suppress the release of histamine induced by antigen at low as well as high levels of release. Based on the data on the effect of fenoterol on IgE-mediated histamine release, it was concluded that in contrast to a human lung mast cell system, the beta-adrenergic receptor-adenylate cyclase system is not a control mechanism in IgE-mediated basophil histamine release. 相似文献
8.
S. S. Kanj J. E. Corkill Z. A. Kanafani G. F. Araj C. A. Hart R. Jaafar G. M. Matar 《Clinical microbiology and infection》2008,14(5):501-504
The prevalence of bla CTX-M , bla TEM and bla SHV genes among extended-spectrum β-lactamase (ESBL)-producing clinical isolates of Escherichia coli ( n = 50) and Klebsiella spp. ( n = 50) from Lebanon was 96%, 57% and 67%, and 40%, 82% and 84%, respectively. Genotyping revealed that the clonal diversity was unrelated to the presence of bla genes. Sequence analysis of 16 selected isolates identified the bla CTX-M-15 , bla TEM-1 , bla OXA-1 and six bla SHV genes, as well as the gene encoding the quinolone-modifying enzyme AAC(6')-Ib-cr. The genes encoding CTX-M-15 and AAC(6')-Ib-cr were carried on a 90-kb plasmid of the pC15-1a or pCTX-15 type, which transferred both ESBL production and quinolone resistance from donors to transconjugants. 相似文献
9.
血管紧张素原基因5′端核心启动子区(-6)A-G和(-20)A-C变异与哈萨克族人原发性高血压相关性分析 总被引:19,自引:0,他引:19
目的 研究血管紧张素源 (angiotensinogen,AGT)基因核心启动子区域 (- 6 ) A- G和 (- 2 0 )A- C位点变异与哈萨克族人原发性高血压相关关系。方法 采用经典的饱和酚 /氯仿抽提法提取哈萨克族正常人 74名和高血压患者 12 5例白细胞基因组 DNA,通过 PCR、单链构象多态性、限制性片段长度多态性和测序等技术 ,鉴定不同个体 AGT基因核心启动子区域 (- 6 )、(- 2 0 )位等位基因的类型 ,观察在高血压组和正常血压组不同基因型的分布和等位基因频率的差异。结果 (1)哈萨克族人 AGT基因 - 16 4~ 73区域仅存在 (- 6 ) A- G、(- 2 0 ) A- C两种变异。(2 ) AGT基因 (- 6 )位点 AA、AG、GG基因型的频率在高血压组和正常血压组分别为 0 .39、0 .4 5、0 .16和 0 .4 9、0 .4 9、0 .0 2 ,两组之间差异存在显著性 (χ2 =8.5 6 ,P=0 .0 14 )。A、G等位基因频率分别为 0 .6 2、0 .38和 0 .73、0 .2 7,差异存在显著性 (χ2 =5 .35 ,P=0 .0 2 1)。(3) AGT基因 (- 2 0 )位点 AA、AC、CC基因型频率在高血压组和正常血压组分别为 0 .6 9、0 .2 6、0 .0 5和 0 .6 5、0 .32、0 .0 3,差异无显著性 (χ2 =2 .4 2 ,P=0 .30 ) ;A、C等位基因的频率分别为 0 .82、0 .18和 0 .82、0 .18,差异无显著性 (χ2 =0 ,P=0 .99)。(4) AGT基 相似文献
10.
Objective To investigate BCL-6 gene mutations in Chinese populations with B-cell non- Hodgkin’s lymphoma.Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5’ noncoding re gion of the BCL-6 gene in a total of 40 cases of diffuse large-cell lymphoma ( DLCL) and follicular lymphoma (FL).Results Nine cases were found to have base substitutions.The incidence of BCL-6 gene mutation and the frequency of single-base changes were approximately 25.7% an d (0.56-1.10)×10(-2)/bp, respectively.Conclusions The 5’ regulatory region of the BCL-6 gene undergoes frequent somatic hypermuta tion during lymphomagenesis and the identification of BCL-6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B-NHL. 相似文献