枸杞多糖对氧化损伤大鼠晶状体上皮细胞凋亡的调控

目的 :研究枸杞多糖 (lyciumbarbarumpolysaccha rides,LBP)对实验性晶状体氧化损伤所致晶状体上皮细胞(lensepithelialcell,LEC)凋亡的影响。方法 :复制大鼠晶状体LEC凋亡模型 ,将 2 4只透明晶状体随机分为 4组 ,空白组、H2 O2 组、白内停组、枸杞多糖组。枸杞多糖组加入终浓度为1g/L的枸杞多糖共同孵育 2 4h ,采用TUNEL法检测LEC凋亡率 ,用透射电子显微镜观察LEC超微结构改变。结果H2 O2 组LEC凋亡率 (92 .0± 2 .5 5 )显著高于空白组 (3.5± 1.84 ) ,(t=6 2 .97,P <0 .0 1) ;枸杞多糖组LEC凋亡率 (16 .6±8.11)与白内停组比较 ,差异有非常显著性 (t=15 .5 0 ,P <0 .0 1)。透射电镜观察 ,H2 O2 组胞浆明显浓缩 ,核内染色质凝聚或成块 ,枸杞多糖组多数LEC形态改变轻微 ,表现为胞浆基质密度轻度升高 ,部分线粒体水肿 ,细胞间间隙增宽。结论 :枸杞多糖可明显抑制LEC凋亡 ,从祖国医药中寻求防治白内障的有效药物具有广阔前景。     

Histamine downregulates CD14 expression via H2 receptors on human monocytes

Lipopolysaccharide (LPS) binds to LPS-binding protein (LBP) in plasma and is delivered to the cell surface receptor CD14 on human monocyte. LPS is transferred to the transmembrane signaling receptor toll-like receptor (TLR) 4. In the present study, the effect of histamine on the expression of CD14 on human monocytes was investigated. Histamine concentration- and time-dependently decreased the expression of cell surface CD14, whereas histamine did not decrease mRNA for CD14 nor increase soluble CD14 (sCD14). The inhibitory effects of histamine on CD14 expression were antagonized by H2-receptor antagonist, but not by H1 and H3/H4 antagonist. The effects of selective H2-receptor agonists, 4-methylhistamine and dimaprit, on CD14 expression mimicked that of histamine indicating that histamine regulated CD14 expression through the stimulation of H2-receptors. The pretreatment with histamine partially inhibited the LPS-induced TNF-alpha production in human peripheral blood mononuclear cells (PBMC). Such inhibition might be due to the down-regulation of CD14 expression on monocytes by histamine.     

两种兔源内毒素结合蛋白的分离、纯化及其体外生物学活性的对比研究

目的　从烧伤合并内毒素血症致多器官损害家兔血清中分离纯化内毒素结合蛋白 ,并初步对比研究它们的生物学活性。方法　兔血清蛋白质通过二步离子交换层析 (Bio Rex 70Resin、Mono Q)及凝胶过滤分离纯化 ,并经流式细胞分析实验、绵羊红细胞凝聚实验、氨基末端氨基酸残基测序等方法鉴定 ,再将所获蛋白质作用于体外培养的人单核细胞 (U937) ,观测其分泌TNFα等细胞因子的生物效应。结果　获得两种具有促进内毒素 (LPS)与外周血单核细胞 (PBMC)结合的蛋白质 ,其中一种相对分子质量为 6 0× 10 4 ,其N端 10个氨基酸序列为TNPGLITRIT ,证实为兔内毒素结合蛋白 (LBP) ;另一种蛋白质相对分子质量为 4 8× 10 4 ,其N端 10个氨基酸序列为GSQGTFTSEE ,与NCBI蛋白质库中的氨基酸序列进行比较 ,未发现相同序列 ,命名为p48。结论　p48与LBP具有相似的生物学功能 ,p48的生物学活性稍弱于LBP。提示体内除LBP外 ,p48也同样起着介导LPS的毒性作用。这可能是近年国外研究中阻断LBP的活性后 ,机体毒性反应并无明显减轻的原因之一。     

耳穴降压沟电脉冲刺激治疗高血压病疗效分析

目的:观察耳穴降压沟电脉冲刺激对高血压病的治疗作用.方法:将129例Ⅰ～Ⅲ期高血压病患者采用华佗自动降压仪取耳穴降压沟电脉冲刺激,每次20～30分钟,每天1次.3疗程(21次)后观察血压变化情况.结果:129例患者,显效44例,有效73例,无效12例,总有效率达90.70%.收缩压平均下降(18.64±1.48)mmHg,舒张压平均下降(8.01±0.68)mmHg,收缩压与舒张压治疗前后比较差异均有极显著性意义(P<0.001).结论:耳穴降压沟电脉冲刺激能有效降低高血压病人的收缩压和舒张压.     

EPR技术研究枸杞多糖清除·OH自由基作用

目的:探讨枸杞多糖清除自由基的作用.方法:采用电子顺磁共振技术(EPR)检测不同浓度枸杞多糖对·OH自由基的清除作用.结果:枸杞多糖清除·OH自由基的能力在0.25 mg/ml为18.64%,在1.0 mg/ml为87.29%,再增大浓度时清除能力呈下降趋势.结论:枸杞多糖低浓度时具显著清除·OH自由基作用,但高浓度时其清除能力却下降.     

Development,validity and reliability of a new pressure air biofeedback device (PAB) for measuring isometric extension strength of the lumbar spine

This study describes the development of a new portable muscle testing device, using air pressure as a biofeedback and strength testing tool. For this purpose, a pressure air biofeedback device (PAB^®) was developed to measure and record the isometric extension strength of the lumbar multifidus muscle in asymptomatic and low back pain (LBP) persons. A total of 42 subjects (age 47.58 years, ±18.58) participated in this study. The validity of PAB^® was assessed by comparing a selected measure, air pressure force in millibar (mb), to a standard criterion; calibrated weights in kilograms (kg) during day-to-day tests. Furthermore, clinical trial-to-trial and day-to-day tests of maximum voluntary isometric contraction (MVIC) of L5 lumbar multifidus were done to compare air pressure force (mb) to electromyography (EMG) in microvolt (μV) and to measure the reliability of PAB^®. A highly significant relationship were found between air pressure output (mb) and calibrated weights (kg). In addition, Pearson correlation calculations showed a significant relationship between PAB^® force (mb) and EMG activity (μV) for all subjects (n?=?42) examined, as well as for the asymptomatic group (n?=?24). No relationship was detected for the LBP group (n?=?18). In terms of lumbar extension strength, we found that asymptomatic subjects were significantly stronger than LBP subjects. The results of the PAB^® test differentiated between LBP and asymptomatic subject’s lumbar isometric extension strength without any risk to the subjects and also indicate that the lumbar isometric extension test with the new PAB^® device is reliable and valid.     

Correlations between pain and function in a longitudinal low back pain cohort

Purpose.?Most studies of low back pain (LBP) and functional limitation have been cross-sectional, and show only modest correlations between pain and function. Though functional limitation may be superior for predicting disability outcomes, there is a need to understand better the gap between pain and function. This study analysed changes in intra-individual correlations between pain and function over time.

Method.?Seventeen men and 16 women currently experiencing LBP provided self-reports of LBP (0-to-10 scale) and functional status (Back Pain Functional Scale) for a maximum of 8 weeks. Spearman correlation coefficients between pain and function scores were calculated for each individual. The effects of pain history, pain intensity, variability and trends over time on pain–function correlations were assessed.

Results.?There were no significant differences in correlation due to gender, age or pain intensity (low versus high). Participants with steeper slopes in change in pain score over the study period had significantly stronger correlations to function than those with weaker trends, r?==??0.91 and r?==??0.45, respectively. Participants with at least one pain-free score during the reporting period had significantly stronger correlations than those with no pain-free reports, r?==??0.80 and r?==??0.51, respectively. Participants having the first episode LBP had stronger correlations (r?==??0.85) than those with persistent symptoms of LBP (r?==??0.62).

Conclusions.?The results suggest that over the course of LBP, within-person pain–function correlations are stronger than those reported in cross-sectional, population-based studies. Changes in pain ratings over time may have more clinical relevance than differences in pain levels between individuals. Among those with more long-standing pain, factors other than pain intensity, such as pain catastrophising or fear avoidant beliefs, may have a greater effect on day-to-day perceptions of functional limitation.     

Benefits of the Restorative Exercise and Strength Training for Operational Resilience and Excellence Yoga Program for Chronic Low Back Pain in Service Members: A Pilot Randomized Controlled Trial

Objective

To examine the feasibility and preliminary effectiveness of an individualized yoga program.

Evidence for Dry Needling in the Management of Myofascial Trigger Points Associated With Low Back Pain: A Systematic Review and Meta-Analysis

Objective

To evaluate the current evidence of the effectiveness of dry needling of myofascial trigger points (MTrPs) associated with low back pain (LBP).

Evaluation of Cognitive Behavioral Interventions and Psychoeducation Implemented by Rehabilitation Specialists to Treat Fear-Avoidance Beliefs in Patients With Low Back Pain: A Systematic Review

Objectives

To systematically locate, critically appraise, and synthesize the available evidence regarding the effectiveness of cognitive behavioral therapies (CBTs) and psychoeducation that can be implemented by rehabilitation specialists to treat fear-avoidance beliefs in patients with acute, subacute, and chronic low back pain (LBP).