Structure,Assembly and Function of Cuticle from Mechanical Perspective with Special Focus on Perianth

This review is devoted to the structure, assembly and function of cuticle. The topics are discussed from the mechanical perspective and whenever the data are available a special attention is paid to the cuticle of perianth organs, i.e., sepals, petals or tepals. The cuticle covering these organs is special in both its structure and function and some of these peculiarities are related to the cuticle mechanics. In particular, strengthening of the perianth surface is often provided by a folded cuticle that functionally resembles profiled plates, while on the surface of the petal epidermis of some plants, the cuticle is the only integral continuous layer. The perianth cuticle is distinguished also by those aspects of its mechanics and development that need further studies. In particular, more investigations are needed to explain the formation and maintenance of cuticle folding, which is typical for the perianth epidermis, and also to elucidate the mechanical properties and behavior of the perianth cuticle in situ. Gaps in our knowledge are partly due to technical problems caused by very small thicknesses of the perianth cuticle but modern tools may help to overcome these obstacles.     

Fold and flexibility: what can proteins' mechanical properties tell us about their folding nucleus?

The determination of a protein''s folding nucleus, i.e. a set of native contacts playing an important role during its folding process, remains an elusive yet essential problem in biochemistry. In this work, we investigate the mechanical properties of 70 protein structures belonging to 14 protein families presenting various folds using coarse-grain Brownian dynamics simulations. The resulting rigidity profiles combined with multiple sequence alignments show that a limited set of rigid residues, which we call the consensus nucleus, occupy conserved positions along the protein sequence. These residues'' side chains form a tight interaction network within the protein''s core, thus making our consensus nuclei potential folding nuclei. A review of experimental and theoretical literature shows that most (above 80%) of these residues were indeed identified as folding nucleus member in earlier studies.     

Insertional re-activation of a chloramphenicol acetyltransferase misfolding mutant protein

The deletion of nine residues from the C-terminus of the bacterialchloramphenicol acetyltransferase (CAT) results in depositionof the mutant protein in cytoplasmic inclusion bodies and lossof chloramphenicol resistance in Escherichia coli. This foldingdefect is relieved by C-terminal fusion of the polypeptide withas few as two residues. Based on these observations, efficientpositive selection for the cloning of DNA fragments has beendemonstrated. The cloning vector encodes a C-terminally truncatedCAT protein. Restriction sites in front of the stop codon allowthe insertion of target DNA, resulting in the production ofproperly folded CAT fusion proteins and regained chloramphenicolresistance. The positive selection of recombinants is accomplishedby growth of transformants on chloramphenicol-containing agarplates. The method appears particularly convenient for the cloningof DNA fragments amplified by the PCR because minimal informationto restore CAT folding can be included in the primers. The cloningof random sequences shows that the folding defect can be relievedby fusion to a wide variety of peptides, providing great flexibilityto the positive selection system. This vector may also contributeto the determination of the role of the C-terminus in CAT folding.     

An 8-bit, 200 MSPS Folding and Interpolating ADC

An 8-bit, 200 MSPS folding and interpolating analog-to-digitalconverter, ADC, has been implemented in a 1.2 µmBiCMOS-process. It achieves 7.5 effective bits with a power dissipationof 575mW. The active area is 4mm². The implementationand measured results are presented. A simple analytical modelfor the interpolation-induced nonlinearity in a folding and interpolatingADC using sinusoidal folding is presented. The bowing of thereference ladder due to interaction with the input stages isanalyzed, and analytical models are derived.     

CAD系统中产品设计三维实体建模方法的研究

三维建模是应用CAD系统进行产品设计的重要步骤。现在市场上已有不少的CAD系统，但是不同系统中的三维建模方法却是基本相同的。在这些三维建模的方法中，往往对用户施加了许多的限制，使得用户无法建立复杂的三维实体。笔者提出了一种新的三维建模方法－折叠法，应用这种方法的概念和原理，可以开发出先进的三维建模CAD系统。折叠二维平面图形构成三维实体是作者提出的一种新概念。     

36 m 高砖砌水塔定向折叠爆破拆除

介绍了复杂环境中砖砌高大水塔的定向折叠爆破拆除.根据周围的环境情况,在施工中采用了开定向窗、挖定位坑等技术措施,确保爆破成功,达到了设计效果.     

基于去调频技术的斜视聚束SAR成像方法

针对具有方位谱混叠现象的斜视聚束式合成孔径雷达(SAR)成像问题,本文研究了一种基于去调频技术的成像方法.文中首先分析了斜视聚束SAR中的方位谱混叠现象,从理论上找出了斜视角影响方位粗聚焦的根本原因.基于分析结果,引入了回波信号非线性相位校正预处理法,彻底消除了斜视角的影响,从而得到正确的粗聚焦结果.然后基于斜视成像模型,推导了预处理回波信号的方位粗聚焦和精聚焦处理过程.在精聚焦的方位滤波处理中,针对非线性相位校正预处理导致的场景聚焦深度限制问题,提出了一种改进的非线性调频变标(NLCS)算法,实现了回波信号的高精度方位压缩.仿真实验结果证明了文中理论分析的正确性和所提方法的有效性.     

柔性标签弯曲及对RFID系统识读距离的影响研究

针对平面结构标签在使用过程中经常发生弯曲形变的问题,研究一种柔性标签发生圆弧形弯曲和垂直折叠形变时天线性能的变化以及对RFID系统识读距离的影响。介绍柔性标签天线的研究现状,分析影响RFID系统识读距离的关键因素,通过示意图阐述弯曲天线模型的建模方法,基于HFSS仿真分析阻抗、增益和辐射效率等的变化规律。通过实际测量验证了RFID系统识读距离的仿真结果及该方法的可行性。     

Designing amino acid sequences to fold with good hydrophobic cores

We present two methods for designing amino acid sequences ofproteins that will fold to have good hydrophobic cores. Giventhe coordinates of the desired target protein or polymer structure,the methods generate sequences of hydrophobic (H) and polar(P) monomers that are intended to fold to these structures.One method designs hydrophobic inside, polar outside; the otherminimizes an energy function in a sequence evolution process.The sequences generated by these methods agree at the levelof 60–80% of the sequence positions in 20 proteins inthe Protein Data Bank. A major challenge in protein design isto create sequences that can fold uniquely, i.e. to a singleconformation rather than to many. While an earlier lattice-basedsequence evolution method was shown not to design unique folders,our method generates unique folders in lattice model tests.These methods may also be useful in designing other types offoldable polymer not based on amino acids     

水力循环澄清池的技术改造

张掖沙湾水厂的水力循环澄清池是上世纪70年代初建造。由于池深，各部分功能不匹配，动能有效利用率低，排泥不畅,对水量、水质变化适应性差，限制了产水量的提高。经强化絮凝、增设斜管、折板等综合性改造后．单池产水量由原来80m^3／d增加到140m^3／d，药耗降低了15％～20％。改造后仅1年多时间．即可从增产水的效益和节省药耗中收回投资。