Ca-DTPA和Zn-DTPA交替使用降低~(238)Pu致大鼠骨肉瘤发生率的效果

本实验用 Ca-DTPA 和 Zn-DTPA 交替促排~(238)Pu,降低大鼠骨肉瘤的发生率,减少 DTPA 促排的副作用。对大鼠肌肉注射3.7×10~4Bq/kg(体重)~(238)Pu2小时后,交替(每隔一日)肌肉注射 Ca-DTPA 和 Zn-DTPA,共促排90天。实验结果表明,促排结束时,促排组骨肉瘤发生率为10%,而对照组为40%;促排46天后,促排组骨、肝和肾中~(238)Pu 滞留量明显低于对照组。     

吸入短寿命氡子体动物肺组织剂量与体重关系的探讨

本文介绍吸入短寿命氡子体的不同种动物肺组织吸收剂量与体重的关系。实验结果表明,吸收剂量随体重的增加而减小。     

~(141)CeO_2经气管内注入后在肺及淋巴结内的动态分布

本文报告了应用γ放射性测量和放射自显影方法,观察~(141)CeO_2经气管内注入后在大鼠肺及局部淋巴结中动态分布的特点。实验结果表明,~(141)Ce 在肺内与在不同部位、不同个体和单个淋巴结中的分布都是很不均匀的;其滞留量随时间而变化。     

大鼠白蛋白酶联免疫分析试剂盒的研制

大鼠白蛋白的相对分子质量约为66 000，而正常大鼠尿液中的大鼠白蛋白含量一般为2～20mg／L，采用酶联分析直接测出正常大鼠尿样中的大鼠白蛋白含量对药物代谢实验具有重要意义。     

对大鼠单次注入氚水后生物效应的观察

大鼠经腹腔一次性注入7.4×10~5Bq·g~(-1)体重氚水后,分别在第1、3、5、7、12、20和35天观察大鼠体重、血象、肝、肾、肺的损伤。结果表明:血象从第1天、肝脏从第3天开始出现损伤,其后逐渐加剧,二者均以第7天最为严重。从第12天开始恢复,到第35天基本恢复正常。在所观察的指标中,以血象及肝脏的病理损伤较为敏感且基本同步,体重的变化不明显。     

多肽配基亲和吸附介质的配基密度控制规律

以谷胱甘肽为配基,琼脂糖微球为骨架,探索将谷胱甘肽通过共价键偶联到琼脂糖微球骨架上,制备可以分离谷胱甘肽S转移酶(Glutathione S-transferase, GST)及以其为标签的融合蛋白的亲和吸附介质.采用正交实验方法考察了谷胱甘肽加入量、偶联缓冲液的pH值和反应温度对亲和介质配基密度的影响.结果发现,该反应过程中的pH值对配基密度影响最大,其次为谷胱甘肽的加入量.用所制备的亲和吸附介质纯化GST(大鼠肝脏谷胱甘肽S转移酶),发现GST的吸附量随配基密度增加而增加,但GST活性却随配基密度的增加而下降,较好的干胶配基密度为260 μmol/g.大鼠肝匀浆液经过离子交换和亲和层析两个步骤,获得了电泳纯的GST,比活力为12.08 U/mg,总活性回收率为40%以上.     

Evaluation of apatite-coated chitosan microspheres for bone regeneration

The apatite-coated chitosan microspheres were fabricated by incubating chitosan in five times simulated body fluid. The apatite deposited on the microspheres was similar to natural bone mineral, as demonstrated by scanning electron microscopy, X-ray diffraction spectra and Fourier transformed-infrared spectroscopy analyses. Rat bone marrow-derived mesenchymal stem cells （BMSCs） were seeded on apatite- coated microspheres to investigate the effect of this scaffold on cell proliferation and differentiation. BMSCs seeded on uncoated microspheres were served as a control. In vivo experiment was evaluated by transplanting the microspheres loaded with or without BMSCs in 5-mm cranial defects of Wistar rats. Bone regeneration was investigated via micro-CT and histological analysis. It was found that apatite-coated chitosan microspheres could significantly promote the proliferation and alkaline phosphatase activity of BMSCs compared with uncoated microspheres. Histological slices and Micro-CT images at 8 weeks revealed much better regeneration of bone in the apatite-coated microspheres loaded with BMSCs than the control. In addition, the defect filled with pure microspheres induced little new bone formation. Our findings suggest that the apatite-coated chitosan microspheres scaffold is a promising carrier of stem cells for cranial bone tissue engineering.     

Enhanced healing of full-thickness diabetic wounds using bioactive glass and Yunnan baiyao ointments

In order to accelerate the chronic wounds healing, we investigated the healing effects of bioactive glass and Yuunan baiyao ointments in streptozotocin-induced diabetic rats. The ointments were prepared by mixing 45S5 bioactive glass powder （16% weight） with Vaseline and different weight percentages of Yurman baiyao. Full-thickness defect wounds were created on the back of 130 SD rats and were randomly divided into 8 groups. The wound healing rates were calculated at 4, 7, 10, 14 and 21 days after surgery. The samples were harvested for further observations. Considering the wound closure rate, group 6 （with 5% Yuunan baiyao） has better wound healing performance than other diabetic groups. The lower inflammatory response was observed by gross observation and confirmed by the results of H＆E staining and TEM observation. Besides, the proliferation of fibroblasts, the formation of granulation tissue, as well as the vascularization, were improved in group 6 compared to other diabetic groups. All results suggest that bioactive glass and Yunnan baiyao ointments can accelerate the recovery of diabetes-impaired skin wounds, and comparing to other diabetic groups, group 6 （with 5% Yunnan baiyao） has better healing effect.     

大鼠刺激器遥控系统的设计与实现

设计并实现一个基于C8051F410和nRF905芯片的大鼠遥控刺激系统。该系统由监控软件、发射器和刺激器构成,运行在PC机上的监控软件可以设置参数并发出控制指令,指令经USB传输进入发射器后被发送出去,刺激器接收指令后产生刺激信号,对大鼠的运动进行控制。刺激脉冲幅值可调,改变刺激参数可实现不同的运动控制。无线通信采用跳频方式,提高了抗干扰性。刺激器体积小、质量轻。实验结果表明,在200 m的范围内,系统工作稳定。