Anticardiolipin Antibody in Patients with Behçet's Disease with and without Vascular Thrombosis

Objective: The clinical value of IgG anticardiolipin antibody in patients with Behçet's disease with or without vascular thrombosis was evaluated.   Methods: IgG isotype of anticardiolipin (aCL) antibody was assessed in 40 Behçet's disease (BD) patients with venous or arterial thrombosis, 40 BD patients without venous or arterial thrombosis and 80 healthy subjects as controls. The levels of IgG aCL were determined by an indirect ELISA method. Color Doppler Sonography, Magnetic Resonance Imaging and conventional angiography were the procedures used for other clinical evaluations. Results: Out of 40 patients with vascular thrombosis, 20(50%) were positive for low to moderate level of IgG aCL. In patients without thrombosis 22(55%) were positive for low to moderate level of IgG aCL while in none (0%) of the healthy subjects the IgG aCL was positive, neither low nor moderate. The number of patients with headache but having a normal cerebral magnetic resonance imaging (MRI), was higher in anticardiolipin positive patients without vascular thrombosis as compared to those with vascular thrombosis, (P = 0.001). Arthritis was noticed in both patents groups. 15% of aCL positive patients without thrombosis had arthritis as compared to none in aCL negative patients without thrombosis (P = 0.02).   Conclusion: The results of this study indicate that although the frequency of IgG aCL was found to be higher in Iranian patients with BD in comparison with the previous reports, except in arthritis the observed elevated IgG aCL does not correlate with clinical disease manifestations, or vascular thrombotic complications.     

A study of DNA bases sequence preference of aclarubicin B to bind DNA and inhibit DNA-dependent RNA synthesis in vitro

采用荧光淬灭法和体外无细胞ＲＮＡ合成系统研究了阿柔比星Ｂ结合ＤＮＡ和抑制ＤＮＡ依赖性ＲＮＡ合成的ＤＮＡ碱基顺序选择性。结果表明阿柔比星Ｂ与小牛胸腺ＤＮＡ，ｐｏｌｙ［ｄ（Ａ－Ｔ）］和ｐｏｌｙ［ｄ（Ｇ－Ｃ）］有明显结合；结合ＲＮＡ的活性小；与天然ＤＮＡ的结合力较与变性ＤＮＡ的结合力大。Ｓｃａｔｃｈａｒ分析显示阿柔比星Ｂ结合３种ＤＮＡ的亲和性依次为ｐｏｌｙ［ｄ（Ａ－Ｔ）］＞ｐｏｌｙ［ｄ（Ｇ－Ｃ）］＞小牛胸腺ＤＮＡ。同样，用大肠杆菌ＲＮＡ多聚酶和大鼠肝细胞核游离ＲＮＡ多聚酶实验均显示阿柔比星Ｂ的抑制力依次为ｐｏｌｙ［ｄ（Ａ－Ｔ）］＞ｐｏｌｙ［ｄ（Ｇ－Ｃ）］＞ｐｏｌｙ［ｄ（Ｉ－Ｃ）］。上述结果证明抑制ＲＮＡ合成的碱基顺序选择性与其结合ＤＮＡ碱基顺序的选择性有关     

Management and outcomes of surgical patients with intestinal Behçet’s disease and Crohn’s disease in southwest China

BACKGROUNDGastrointestinal involvement in Behçet''s disease (GIBD) and Crohn’s disease (CD) are inflammatory diseases sharing a considerable number of similarities. However, different from CD, the operative and postoperative management of GIBD remains largely empirical because of the lack of comprehensive treatment guidelines.AIMTo compare surgical patients with GIBD and those with CD in a medical center and identify notable clinical features and effective postoperative treatment for surgical patients with GIBD.METHODSWe searched patients diagnosed with CD and GIBD who underwent operations for gastrointestinal complications from 2009 to 2015 at West China Hospital of Sichuan University. A total of 10 surgical patients with GIBD and 106 surgical patients with CD were recruited. Information including demographic data, medication, and operative and postoperative parameters were collected and analyzed. As the incidence of surgical GIBD is low, their detailed medical records were reviewed and compared to previous studies. Moreover, the prognoses of CD and GIBD were evaluated respectively between groups treated with biological and non-biological agents.RESULTSIndication for first surgery was often acute intestinal perforation for GIBD patients (7/10 vs 0/106, P < 0.001), whereas intestinal fistulae (0/10 vs 44/106, P = 0.013) and ileus (0/10 vs 40/106, P = 0.015) were the indications for surgical CD patients. Approximately 40% of patients with GIBD and 23.6% of patients with CD developed postoperative complications, 50% of patients with GIBD and 38.7% of patients with CD had recurrence postoperatively, and 40% (4/10) of patients with GIBD and 26.4% (28/106) of patients with CD underwent reoperations. The average period of postoperative recurrence was 7.87 mo in patients with Behçet''s disease (BD) and 10.43 mo in patients with CD, whereas the mean duration from first surgery to reoperation was 5.75 mo in BD patients and 18.04 mo in CD patients. Surgical patients with GIBD more often used corticosteroids (6/10 vs 7/106, P < 0.001) and thalidomide (7/10 vs 9/106, P < 0.001) postoperatively, whereas surgical patients with CD often used infliximab (27/106), azathioprine, or 6-mercaptopurine (74/106) for maintenance therapy.CONCLUSIONPatients suffering GIBD require surgery mostly under emergency situations, which may be more susceptible to recurrence and reoperation and need more aggressive postoperative treatment than patients with CD.