白斑综合症病毒(WSSV)的宿主调查

用地高辛 (DIG)标记的WSSVDNA探针斑点杂交与原位杂交技术 ,在中国对虾、斑节对虾、南美白对虾、刀额新对虾、脊尾白虾、天津厚蟹、日本大眼蟹体内检测到了WSSV ,它们是WSSV的天然宿主 ;在经人工感染的哈氏美人虾、短脊鼓虾、克氏原螯虾、肉球近方蟹、滕壶体内检测到了WSSV ;在球形侧腕水母、病虾池的桡足类等浮游生物、卤虫无节幼体以及人工浸泡感染卤虫成体体内没有检测到WSSV。经原位杂交检测 ,虾类的甲壳下上皮、胃上皮、附肢、造血组织、鳃等组织器官均可被WSSV侵染 ,其中甲壳下上皮和鳃对WSSV敏感 ;蟹类的甲壳下上皮和鳃对WSSV敏感 ;在中国对虾、南美白对虾、脊尾白虾、注射感染的克氏原螯虾的精巢中 ,精荚的结缔组织细胞和血细胞呈阳性 ,在中国对虾、脊尾白虾以及注射感染的短脊鼓虾的卵巢中 ,结缔组织细胞和滤泡细胞被WSSV感染。     

THE INTRINSIC ACIDITY CONSTANTS AND SPECIFIC SURFACE AREA OF MARINE SOLID PARTICLESII. THE RELATIONSHIP BETWEEN THE INTRINSIC ACIDITY CONSTANT AND THE TERNARY SURFACE COMPLEX

This paper deals with the infrared spectra of " amino acid- clay , calcium carbonate and y-AlOOH" and " Cu (II )-clay-amino acid" model systems, and shows that the model of the ternary surface complex is M-OHLCu (L = amino acid) for marine solid particle-Cu (II)-amino acid. Study of the formation mechanism of the ternary surface complex shows that the specific surface area , and especially the intrinsic acidity constant, determine whether the ternary surface complex is easily formed, and that factor, FTSC,quantifies the relationship between the promoting effect of organics on Mt-marine solid particle ion exchange and the intrinsic acidity constant and specific surface area.     

Budd—Chiari综合征的CT评价

回顾性分析了５２例Ｂｕｄｄ－Ｃｈｉａｒｉ综合征的ＣＴ表现，ＣＴ显示肝尾叶增大５０例（９６％），肝脏呈低度密度或密度不均，４２例（８１％）肝静脉未明显，２２例（４２％）见有肝内侧枝血管，４９例（９４％）有肝外侧枝血管，作者认为ＣＴ检查对Ｂｕｄｄ－Ｃｈｉａｒｉ综合征有较高的诊断价值。     

Under stress permeability determination of the Meuse/Haute-Marne mudstone

In the underground waste isolation projects such as the ANDRA'one in the site of Bure, the transport properties of the surrounding rock mass is of fundamental importance. To measure very low permeability, we use the modified version of the pulse test proposed by Hsieh et al. [Hsieh, P.A., Tracy, J.V., Neuzil, C.E., Bredehoeft, J.D., Silliman, S.E., 1981. A transient laboratory method for determining the hydraulic properties of ‘tight’ rocks — I. Theory. Int. J. Rock Mech. Min. Sci. Geomech. Abstr. Vol. 18, pp. 245-252] which enables the intrinsic permeability, k, and the specific storage coefficient, S_s, of rocks such as mudstone to be characterized. In this paper, the special effort performed on the laboratory apparatus design, to ensure a good sensitivity of the rock response with respect to both parameters, k and S_s, is presented. In addition, two parameters identification procedures are proposed: the graphical method given by Hsieh et al. [Hsieh, P.A., Tracy, J.V., Neuzil, C.E., Bredehoeft, J.D., Silliman, S.E., 1981. A transient laboratory method for determining the hydraulic properties of ‘tight’ rocks — I. Theory. Int. J. Rock Mech. Min. Sci. Geomech. Abstr. Vol. 18, pp. 245-252] and a parameter identification based on the solution of an inverse problem. The efficiency of the apparatus design and the parameters identification procedures is then demonstrated though some pulse tests performed on the Meuse/Haute-Marne mudstone.     

痛泻要方对溃疡性结肠炎患者中医证候及肠道菌群的影响

目的：观察痛泻要方对溃疡性结肠炎（UC）肝郁脾虚证的临床疗效及其对肠道菌群的影响。方法：将40例UC肝郁脾虚证患者随机分为治疗组和对照组,每组各20例。对照组予美沙拉嗪肠溶片治疗,治疗组在对照组基础上加用痛泻要方治疗。2组疗程均为12周,比较2组治疗前后中医证候积分及肠道菌群的变化。结果：治疗前,2组中医证候积分、主要肠道菌群数目比较,差异无统计学意义（P＞0.05）。与治疗前比较,治疗后2组中医证候积分均降低,有益菌群（双歧杆菌、乳酸杆菌）数目均明显上升,而有害菌群（大肠埃希菌）数目明显下降,且治疗组的指标改善情况均优于对照组,差异有统计学意义（均P<0.05）。结论：痛泻要方可有效改善UC肝郁脾虚证患者的中医证候,纠正患者肠道菌群的紊乱,提高临床疗效。     

Time–frequency representation of earthquake accelerograms and inelastic structural response records using the adaptive chirplet decomposition and empirical mode decomposition

In this paper, the adaptive chirplet decomposition combined with the Wigner-Ville transform and the empirical mode decomposition combined with the Hilbert transform are employed to process various non-stationary signals (strong ground motions and structural responses). The efficacy of these two adaptive techniques for capturing the temporal evolution of the frequency content of specific seismic signals is assessed. In this respect, two near-field and two far-field seismic accelerograms are analyzed. Further, a similar analysis is performed for records pertaining to the response of a 20-story steel frame benchmark building excited by one of the four accelerograms scaled by appropriate factors to simulate undamaged and severely damaged conditions for the structure. It is shown that the derived joint time–frequency representations of the response time histories capture quite effectively the influence of non-linearity on the variation of the effective natural frequencies of a structural system during the evolution of a seismic event; in this context, tracing the mean instantaneous frequency of records of critical structural responses is adopted.The study suggests, overall, that the aforementioned techniques are quite viable tools for detecting and monitoring damage to constructed facilities exposed to seismic excitations.     

Main concepts of the "European approach" to karst-groundwater-vulnerability assessment and mapping

In order to achieve some consistency in the establishment of groundwater intrinsic vulnerability maps in Europe, a new approach is proposed by Working Group 1 of the European COST Action 620 on "Vulnerability mapping for the protection of carbonate (karst) aquifers". A general procedure is offered which provides consistency while allowing the required flexibility for application to a continent and under conditions of varying geology, scale, information availability, time, and resources. The proposed methodology is designed to be clearly more physically based than the existing vulnerability-mapping techniques. It takes the specificity of the karstic environments into account without necessarily excluding the applicability to other geological conditions. Combined "core factors" for overlying layers and for concentration of flow account for the relative protection of groundwater from contamination while taking into account any bypass of the overlying layers. A precipitation factor is distinguished for describing characteristics of the input of water to the system. Differentiation is made between groundwater resource intrinsic vulnerability mapping and source intrinsic vulnerability mapping. For the latter, a factor describing the karst network development is relevant. This short technical note describes a first step in the work program of Working Group 1 of the COST Action 620. Future steps are now in progress to quantify the approach and to apply it in various European pilot areas. Electronic Publication     

White spot syndrome virus envelope protein VP124 involved in the virus infection

White spot syndrome virus （WSSV） is one of the major shrimp pathogens causing large economic losses to shrimp farming. In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins （VP39, VP124 and VP187 ）, individually. And then they were injected intramuscularly into crayfish （Procambarus clarkii） to conduct in vivo neutralization assays. The results showed that for groups injected with virions only and groups injected with the mixture of virions and antiserum against VP124, the crayfish mortalities were 100% and 60% on the 8th day postinfection, individually. The virus infection could be delayed or neutralized by antibody against the envelope protein VP124. Quantitative PCR was used to further investigate the influence of three antisera described above on the virus infection. The results showed that the antiserum against VP124 could restrain the propagation of WSSV in crayfish. All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection.     

经验模态分解和独立分量分解在航磁数据处理中的应用

以东天山1∶20万航磁数据为例,利用经验模态分解(EMD)方法对航磁数据进行分解,然后利用独立分量分析(ICA)方法对分解出的固有模态函数(IMF)数据进行重构。分解出来的从高频到低频IMF与地质体的分布具有一定的相关性,不同频率的IMF代表了一定的地质意义。经过ICA重构后的独立分量(IC)能够对异常IMF函数从盲源分离的角度进行有效的重构,其结果可以在一定程度上解释不同的地质事件对磁异常的贡献,并对构造识别和构造区域划分具有一定的指示意义。     

虾蟹混养塘中WSSV对三疣梭子蟹(Portunus trituberculatus)致死效应的定量研究

采用实时荧光定量PCR法,在对白斑综合症多发的虾蟹混养塘内三疣梭子蟹体内病毒含量进行定量跟踪检测的基础上,结合电子显微镜及病理切片,对室内经口服、注射两种回感模式处理已感染白斑综合症病毒的濒死三疣梭子蟹体内病毒含量进行实验验证,并观察其体内主要器官组织的病理改变。结果表明,受白斑综合症病毒感染的三疣梭子蟹存活与死亡取决于蟹体内病毒含量,当病毒含量≥109拷贝/ml时均进入濒死或死亡阶段,其主要器官组织均有明显的病理改变。