苯乳酸抑制链格孢霉作用靶位的分析

以从自然腐败的樱桃上分离的链格孢霉（Alternaria sp.）LD3.0086为指示菌，研究苯乳酸对链格孢霉的主要抑制作用靶位。应用分光光度法测定苯乳酸对链格孢霉的最小抑菌浓度，通过卡尔科弗卢尔荧光增白剂染液（calcofluor white，CFW）染色观察苯乳酸对菌丝顶端生长的破坏作用，利用扫描电子显微镜和透射电子显微镜观察链格孢霉的超微结构变化，通过测定苯乳酸作用前后链格孢霉上清液中N-乙酰葡萄糖胺质量浓度变化研究苯乳酸对菌丝细胞壁的破坏作用，应用荧光双染色法观察苯乳酸对链格孢霉菌丝细胞膜的损伤作用。结果表明，12.5 mmol/L的苯乳酸能有效抑制链格孢霉的生长；与对照组（无菌水处理）相比，苯乳酸处理后链格孢霉顶端生长细胞无明显形变，经12.5 mmol/L苯乳酸处理的链格孢霉上清液中N-乙酰葡萄糖胺质量浓度基本不变；苯乳酸处理24 h，链格孢霉菌丝细胞壁表面无明显损伤，细胞内结构发生明显变化；苯乳酸短时间（4 h）处理链格孢霉，菌丝细胞膜仍较为完整，加入苯乳酸较长时间（8 h）后细胞膜发生破裂。综合分析可知，苯乳酸对链格孢霉的主要作用靶位应不是菌丝体的细胞壁和细胞膜，而是在菌丝体内部，通过破坏菌丝内部细胞器结构或引起细胞内的生化反应，从而抑制链格孢霉的生长和繁殖，发挥抑菌活性。     

Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His₆-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2.     

Site-Specific PEGylation of Therapeutic Proteins

The use of proteins as therapeutics has a long history and is becoming ever more common in modern medicine. While the number of protein-based drugs is growing every year, significant problems still remain with their use. Among these problems are rapid degradation and excretion from patients, thus requiring frequent dosing, which in turn increases the chances for an immunological response as well as increasing the cost of therapy. One of the main strategies to alleviate these problems is to link a polyethylene glycol (PEG) group to the protein of interest. This process, called PEGylation, has grown dramatically in recent years resulting in several approved drugs. Installing a single PEG chain at a defined site in a protein is challenging. Recently, there is has been considerable research into various methods for the site-specific PEGylation of proteins. This review seeks to summarize that work and provide background and context for how site-specific PEGylation is performed. After introducing the topic of site-specific PEGylation, recent developments using chemical methods are described. That is followed by a more extensive discussion of bioorthogonal reactions and enzymatic labeling.     

Factors involved in associations between Facebook use and college adjustment: Social competence,perceived usefulness,and use patterns

Although previous research has investigated widespread use of social media, especially Facebook, by youth attending college, the conditions under which these media foster adjustment to college remain unclear. This study tested a model illuminating pathways linking social competence to college adjustment via students’ perceptions about the usefulness of Facebook and ways in which they used the medium. Self-report survey data from 321 college students (M age = 20.09; 58% female; 84% Caucasian) attending a major Midwestern university supported the proposed model, indicating that higher social competence could foster or impede college adjustment, depending upon how it was related to beliefs about the usefulness of different Facebook functions and how these perceptions, in turn, were associated with patterns of Facebook use. Findings underscore the importance of considering connections among personal attributes, perception of media effectiveness, and media behaviors in assessing the implications of social media for users’ psychosocial well-being.     

富平银沟遗址青瓷标本的类型学分析

富平银沟遗址位于陕西省渭南市富平县华朱乡东北部银沟村。经过为期两年的考古调查与勘探,在该地区发现和搜集了大量的精美陶瓷产品。其中青瓷产品占比最大,具有很高的研究价值。本文主要通过选取银沟地区青瓷器形较为完整,并具有典型代表性的青瓷标本进行类型学的整理分析。根据器形,青瓷标本可分为碗、盘、盒、钵、香炉、瓶、罐、壶等。通过与耀州窑典型青瓷器的器形与纹饰对比,从器物的类型学解析银沟遗址青瓷器的烧造年代时期。     

一种大尺度二维直线度精密测量的新方法

本文提出了一种远距离、大范围二维直线度测量的新方法．该方法采用特殊设计的十字线准直激光器及线阵CCD，通过特殊的光学系统结构设计，能在0-20m距离内连续测量，直线度范围可达70mm，精度优于0．1mm，实际测量结果表明，该方法可适应野外作业环境。     

基于JSP技术的Web网站安全性研究

通过对基于JSP技术的Web网站的体系结构的介绍．从Web服务器安全、JSP脚本编程及数据库安全等几方面分析研究了web网站存在的安全问题，并探讨了web网站开发时应采取的安全措施。     

钻机用集成化液压源的研制

针对当前钻机液压配套设备越来越多的趋势,研制了集成化液压源。集成化液压源不是把原有的几个液压源的叠加组合,而是实现了液压源电气就地控制和远程控制、流量集中控制分配,压力集中控制,形成多种输出流量、多级输出压力和多个输出端口。     

浅谈水利枢纽道路工程建设中的几个问题及其对策

从水利枢纽工程建设的实际出发,分析了枢纽道路建设中的几个值得考虑的基本问题。针对永久性道路的建设环境和建筑特点以及临时性道路的复杂性和多样性,提出了永久性道路和临时性道路建设的基本对策。基于高新技术材料HEC作为道路建筑胶凝材料的实际工程应用,在道路结构布局观念、设计思想、建筑选材和施工工艺及其技术经济性能比等方面提出了枢纽道路工程建设的实施方案,其有效性和可推广性在水布垭道路工程建设中得到了印证。     

摩擦强度对薄膜表面形态的作用:原子力显微镜下的观察

展示了摩擦强度对聚酰亚胺薄膜表面形态的影响，原子力显微图像显示，机械摩擦会使聚酰亚胺薄膜表面上形成微沟槽，这些沟槽的表面具有丰富的表面精细构造。原子显微图像还揭示了机械摩擦可以改变被磨擦聚酰亚胺膜的表面形态。