Probing molecular-level transformations of dissolved organic matter: insights on photochemical degradation and protozoan modification of DOM from electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Molecular-level characterization of natural organic matter (NOM) has been elusive due to the inherent complexity of natural organic mixtures and to the fact that individual components are often polar and macromolecular. Electrospray ionization (ESI) is a “soft” ionization technique that ionizes polar compounds from aqueous solution prior to injection into a mass spectrometer. The highest resolution and mass accuracy of compounds within NOM have been achieved when ESI is combined with an ultrahigh-resolution mass spectrometer such as the Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS). With this technique, individual molecules within a variety of natural organic mixtures can be detected and their elemental composition can be determined. At low mass-to-charge (m/z) ratio, the resolution is high enough to assign exact molecular formulas allowing specific components of these mixtures to be identified. In addition to molecular identification, we can now use ESI FT-ICR MS to examine molecular-level changes in different organic mixtures as a function of relevant geo-processes, such as microbial alterations and photochemistry. Here we present the results from the application of ESI FT-ICR MS to two geochemical questions: (1) the effect of photoirradiation on the molecular composition of fulvic acids and (2) the role of protozoan grazers in the modification of DOM in aquatic systems.     

海水中低含量铵氮的高灵敏度荧光法测定

提出了一种改进的、可测定海水中低含量铵氮的高灵敏度荧光分析方法。其原理是在硼酸缓冲溶液的作用下,海水中的铵氮与邻苯二甲醛(OPA)发生衍生化反应,通过测定生成的荧光产物来确定水体中铵氮的浓度。方法的检测限极低(0.002 5μmol/L),重现性好,且水样用量少(10 mL),适用于海水中溶解有机物的光铵化研究及其他低铵含量水样的测定。     

Study of macroaggregate composition using FT-IR and H-NMR spectroscopy

Mucous macroaggregates, recently observed in the northern Adriatic in summer of 1997 and late spring of 2000, are primarily the product of phytoplankton (diatom) exudates during favourable environmental conditions in late spring, and can be viewed as macrogels. The FT-IR and ¹H-NMR spectra of macroaggregate samples from the northern Adriatic, collected in different formation stages in July 1997, August 1997, September 1997 and June 2000, as well as of cultured diatom Skeletonema costatum, showed that they are similar and composed of aliphatic components and polysaccharides bonded through carboxylic and amide groups, and organosilicon compounds. The stability of macrogels of macroaggregates is, according to FT-IR and X-ray analyses, most probably enhanced by interactions with entrapped particles of calcite, quartz and clay minerals. These organic-mineral associations seem to be important for mucous phenomena. According to ¹H-NMR spectra, the temporal differences in macroaggregate composition show a relative increasing percentage of organosilicon compounds and aliphatic components bonded to carbohydrates through ester and amide groups, and an increasing ratio between aliphatic structures and carbohydrates. This indicates that aliphatic chains bonded to Si and carbohydrates may contribute to the persistence and stability of macroaggregates in the summer stratified waters in the northern Adriatic, while the temporal decrease of carbohydrate content is most probably due to microbial and photochemical degradation of algal reserve polysaccharides.     

国家西部测图工程产品质检系统的设计与实现

质量检验手段一直是实施数字测绘产品质量控制的瓶颈问题。本文论述了国家西部测图工程产品质检系统的设计与实现,为数字线划图(DLG)、数字高程模型(DEM)、数字正射影像图(DOM)等数字测绘产品质量检验提供了有效的工具和手段,为西部测图工程项目高效优质的实施提供保障。     

基于GDI+的GML可视化模型设计

本文介绍了基于GML地理数据的可视化模型设计方法。利用文档对象模型(DOM)对GML进行解析,以及通过对GD I+自身特性的分析,展示了在.NET环境下采用GD I+类库进行可视化模型设计的优良特性,为实现GML数据可视化提供了一个切实可行的方案。     

基于不同GCP数量和获取方法的数字正射影像的制作与评定

SPOT-5影像的高分辨率和高信息量的性质,使其得到了广泛的应用,特别是在全国第二次土地利用调查中得到了更加深入的应用。而数字正射影像的制作是SPOT-5遥感影像应用的关键所在。本文在介绍DOM制作的基础上,依据当前获取GCP主要的三种途径采用ERDAS遥感图像处理软件进行了实验,并对最终的结果精度进行分析,总结了DOM制作过程的相关问题。     

QuickBird影像制作1：10000DOM方法探讨

随着卫星遥感影像数据更加广泛地应用于测绘,针对QuickBird影像制作1：10000 DOM的方法,从正射纠正的三种不同方式进行了实验和探讨,分析了利用外业控制点或者在原有DRG、DOM上选取控制点制作DOM的方法、精度、工作量,总结提出了方便、快捷、实用的工作模式。     

基于SVG的地理信息编码与DOM交互解析实现

SVG作为新兴的基于XML的矢量图形描述规范，在很多方面与地理信息系统中的基本概念表述一致。在对地理信息进行SVG编码的基础上，利用DOM对地理信息SVG编码文档进行解析，实现了基于SVG的Web地理信息发布实例。实验表明，采用SVG技术的通用性好、矢量显示质量高、响应速度快的优点来开发地理信息的Web应用，也是顺应Web地理信息发布发展需要的。     

Enhanced red fluorescence emission in the oxygen minimum zone of the Arabian Sea

 Depth profiles of fluorescence at several excitation and emission wavelengths were measured along with CTD data during the cruise So119 of RV Sonne in the Arabian Sea from 12 May to 10 June 1997. In addition to chlorophyll fluorescence from phytoplankton in the near-surface layer, the profiles in the oxygen minimum region well below the euphotic zone show enhanced red fluorescence. Red fluorescence intensity is inversely related to the oxygen concentration in intermediate and deep waters. A relationship to characteristic water masses of the region cannot be found in the data, and this holds also with chemical data such as DOC. Absorbance spectra of water samples taken in the oxygen minimum zone show an absorption band at 420 nm wavelength with about 50 nm bandwidth, much weaker than gelbstoff absorbance in the same wavelength range. The absorption band remains stable after sample filtration with 0.45 μm glass fibre filters. Hence, the size of the absorbing matter is in the range of dissolved molecules or particles much smaller than 1 μm. Fluorescence spectra of unfiltered samples with 420 nm excitation show a weak emission band at 600 nm and a more pronounced one at 660 nm wavelength. The trailing edge of the 660 nm band falls into the range of chlorophyll emission, thus giving rise to the observed depth profiles of red fluorescence in the oxygen minimum zone. Red fluorescence measured on samples remain stable during a few hours after sampling even in the presence of oxygen. It is not detectable after several weeks of sample storage in the dark and cannot be reproduced even after depletion of dissolved oxygen. Received: 22 May 2002 / Accepted: 18 February 2003 Responsible Editor: Andreas Oschlies Acknowledgements. This work was supported by a grant from the Federal Minister of Education and Technology, Bonn, within the frame work of the JGOFS Arabian Sea program. We are grateful to the captain and the crew of RV Sonne for their support. We are indebted to Mrs. Kirsten Neumann from the Institute of Marine Chemistry and Biogeochemistry of the University of Hamburg for providing the oxygen data, and to Mr. Nikolai Delling from the same institute for making the DOC and chlorophyll data available to us.