Effect comparison between sacral blocking and massage combined with traction on protrusion of lumbar intervertebral disc

Background:Epidural injection is an important method in treatment of protrusion of lumbar intervertebral disc.Especially in the recent decade sacral injection is adopted more and more often.Objective:To compare the clinical effects of sacral blocking and massage combined with traction.Unit:Yinhe Hospital of Beijing.Subjects:290 outpatients with grade Ⅱ of ASAI were selected from May,1994 to July,2000.Patients were randomly divided into observation group(n=145)and control group(n=145).Sacral blocking was adopted in observation group and 80 males and 65 females aged 20-78(mean:46.4)years old were included with disease course 3 days to 30 years.Massage combined with treaction was adopted in control group and 74 males and 71 females aged 22-74(mean:44.9)years oled were inmcluded with disease course 2 days to 10 years.Interention:Sacral blocking:Operation was according to sacral blocking and druge(dexamethasone injection,2 mg;0.75% bupivavaine hydrochloride,5 ml;saline,50ml) were injected slowly after success of puncture.Patient rested for 0.5h after injection and left treatment room if no adverse effects occurred.Massage combined with traction:Massage was adopted after traction to every patients.(1)Massage:Patient was seated at square stool and lower limbs separated naturally with same width of shoulders.Doctors sit behind patient.First,thubs of both hands were pressed on projecting part near spinous process(pressing points were selected according to different projecting part).Flexing and extending forward and backward were taken at the same time and extent was ddecided addording to patients‘ maximal endurable degree,5-10 timmes.Upper part of body was turned 45 degrees to left and right.Massage was still taken to affected part,3-t times.(2)Traction:Pelvis was extracted with general physical therapy and massage table.Supine position,chest and treaction bandage were fixed,tractiron wheel was turned slowly until patient felt comfortable with maximal endurance as limit(traction force,20-40kg),After extracting for 5 minutes,massagee switch was turned on to take rolling massage to bck and both lower libmbs.Traction force was increased every5 minutes and limit was still according to patient‘s maximal endurance,30 minutes for every time,once a day and ten times as a therapeutic course.Cohclusion:Effect of sacral blocking on protrusion of lumbar intervertebral disc was positive and fewer therapeutic time were needed.Massage combined with traction was also effective,safe and no adverse effects wer observed,but more therapeutic times were neeeded and inconveinient.     

Na^＋／H^＋交换器-1抑制诱导大鼠肺动脉平滑肌细胞凋亡的可能机制

背景：肺动脉平滑肌细胞(pulmonary artery smooth muscle cells，PASMC)的增殖在缺氧肺动脉高压肺血管重构中起重要作用。解放军第三军医大学附属新桥医院全军呼吸研究所在前期实验中通过构建Na^+／H^+交换器-1(sodium／pmton exchanger isofom-1，Na^+／H^+exchanger isoflorm-1，Na^+／H^+ antiporter isoform-1，NHE-1)特异性核酶基因逆转录病毒载体，转染入体外培养的大鼠PASMC内表达，发现NHE-1抑制可诱导细胞内酸化而抑制PASMC增殖，并促进其凋亡。但这种促凋亡作用是通过什么途径来完成，尚不清楚。目的：探讨Bcl-2、Bax蛋白表达在NHE-1抑制而诱导大鼠PASMC凋亡中的作用。设计：分组对照实验。地点和材料：实验在解放军第三军医大学附属新桥医院全军呼吸内科研究所完成。转染表达NHE-1特异性核酶基因的体外培养大鼠PASMC(PRZ细胞)、转染PLXSN空载体的大鼠PASMC(PX细胞)、未处理大鼠PASMC细胞(PA细胞)为前期实验所制备。干预：已构建NHE-1特异性核酶基因逆转录病毒载体，转染入体外培养的大鼠PASMC内表达，同时转染pLXSN空载体入另一组大鼠PASMC内，后者与未转染的大鼠PASMC细胞为对照组。用荧光指示剂(Fura-2／AM)测定法检测转染NHE-1特异性核酶基因的大鼠PASMC内Ca^2+([Ca^2+]i)变化；RT-PCR方法检测细胞内Bcl-2和Bax mRNA表达变化，免疫组化法检测细胞内Bcl-2和Bax蛋白表达变化。主要观察指标：①转染NHE-1特异性核酶基因的大鼠PASMC内Ca^2+([Ca^2+]i)变化。②细胞内Bcl-2和Bax mRNA表达变化。③细胞内Bcl-2和Bax蛋白表达变化。结果：转染NHE-1特异性核酶基因后，大鼠PASMC内[Ca^2+]i显著升高，细胞内[Ca^2+]i在PA细胞[(95．94&;#177;6．39)nmol／L]与PX细胞[(98．08&;#177;7．37)nmol／L]之间差异无显著性意义(P&;gt;0．05)，而PRZ细胞[(198．08&;#177;16．59)nmol／L]显著高于PA细胞及PX细胞，差异有显著性意义(P&;lt;0．001)。Bcl-2 mRNA及蛋白表达显著降低(PA细胞、PX细胞、PRZ细胞的平均积分光密度分别为2．21&;#177;0．18，2．09&;#177;0．30。1．45&;#177;0．20)，Bax mRNA和蛋白表达显著增加(PA细胞、PX细胞、PRZ细胞的ABax／Aβ-actin分别为0．17&;#177;0．02，0．23&;#177;0．06，0．59&;#177;0．08)。结论：NHE-1抑制诱导的PASMC凋亡与[Ca^2+]i增加、Bcl-2表达降低及Bax表达增加有关。     

Brugada综合征1例报告

患者男,74岁。因上腹部隐痛10d、柏油样便4d,于1999年9月9日入院。查体:体温37.1℃,脉搏98次/min,血压105/60mmHg,心肺无异常,腹平软,剑突下深压痛。血液生化及电解质无异常发现,纤维胃镜检查示十二指肠球部溃疡,X线胸片,心脏彩色多谱勒超声心动图示心脏结构及功能正常。心电图示窦性心律,不完全性右束支阻滞伴ST段抬高(V_1～V_3呈马鞍形抬高),P-R间期、Q-T间期、QTc间期均     

改水地区骨干人群健康相关知识、态度、行为状况调查分析

通过 1998～ 2 0 0 2年在河北、湖北、江西、内蒙古和云南 5省 (区 )实施世界银行贷款第三期中国农村供水与环境卫生项目 ,对项目村骨干人群在项目实施前进行深入访谈研究 ,了解项目实施地区健康教育环境支持条件 ,分析当地开展健康教育的资源状况 ,及农村骨干人群参与此项活动的意愿、要求和建议。对象与方法1 对象　 2 0个项目村骨干人群 :村主任或支书、村妇女主任、村医生和小学教师共 75名。2 方法　每个项目省随机选择 2个具有代表性的项目县 ,每个项目县选取 2个有代表性的项目村 ,对其骨干人群进行调查。3 内容　项目村骨干人群对…     

Effect comparison between sacral blocking and massage combined with traction on protrusion of lumbar intervertebral disc

Background:Epiduralinjectionisanimportantmethodintreatmentofprotrusionoflumbarintervertebraldisc.Especiallyintherecentdecadesacralinjectionisadoptedmoreandmoreoften.Objecive:Tocomparetheclinicaleffectsofsacralblockingandmassagecombinedwithtraction.Unit:YinheHospitalofBeijing.Subjects:290outpatientswithgradeIIofASAIwereselectedfromMay,1994toJuly,2000.Patientswererandomlydividedintoobservationgroup(n=145)andcontrolgroup(n=145).Sacralblockingwasadoptedinobservationgroupand80malesand65female…     

腹股沟区的解剖学观察

1 材料与方法1.1 80具尸体(成尸50具、童尸30具皆为男性)经福尔马林防腐固定,上述尸体均系局解手术学教学用尸体.1.2在解剖160侧腹股沟区时,将腹外斜肌腱膜从髂前上棘水平切至腹直肌外缘,然后沿外缘切至耻骨结节,将腹外斜肌腱膜翻向外下方;观察腹股沟     

自拟降糖饮治疗糖尿病100观察

我们1984～1989年,用自拟降糖饮治疗糖尿病100例,并与单用西药治疗的82例患者进行疗效观察,收效尚满意,现总结如下。临床资料一、一般料料将所观察的182例病人,按随机分配表法,分成用降糖饮的治疗组100例和单用西药的对照组82例。其中治疗组100例中,男48例;女52例。年龄:22～67岁,平均56岁;病程6个月～14年,平均6年。对照组     

人肺癌细胞转录因子--C/EBP和HMG样蛋白的EMSA检测

目的：检测人肺癌细胞中是否存在与Na^ /H^ 交换蛋白-1（NHE-1）基因转录相关的重要转录因子--C/EBP和HMG样蛋白。方法：采用电泳迁移率改变分析法（EMSA）检测人肺癌细胞株核蛋白中C/EBP和HMG样蛋白的表达及其水平。结果：EMSA检测到A549细胞和转染NHE-1反义载体的A549细胞均有转录因子C/EBP和HMG样蛋白的表达，且后者表达水平高于前者；采用50倍未标记的片段能完全抑制相应转录因子与^32P标记的相同片段的结合。结论：A549细胞核蛋白中存在能与C/EBP结合序列和Poly(dA：dT)区结合的转录因子，即C/EBP和HMG样蛋白，后者的表达水平高于前者。特异性竞争抑制试验表明外源性特异DNA片段能抑制相同片段与转录因子的结合。     

中医灸与人体穴位红外辐射光谱特性研究

本文通过锁相放大技术测到人体不到1cm^2面积的体表红外辐射光谱。通过对这些光谱的归一化处理后发现，不同人体和同一人体的不同穴位，尽管其红外辐射绝对光谱及强度相差很大，但归一化光谱都有相当的一致性与可比性。进一步将人体发射的平均归一化光谱与中医艾条灸、隔姜灸、隔蒜灸及隔附子饼灸点燃过程中的光谱比较发现，后面三种间隔灸与人体自发辐射的光谱有着惊人的一致性，而艾条灸等的光谱却相差甚远。