吗啡、甲啡肽和强啡肽在镇痛中的相互作用及阿片拮抗剂纳洛酮的阻断作用

本重点观察了阿片拮抗剂纳洛酮阻断上述阿片类物质镇痛作用所需的剂量。     

CCK receptor antagonist L-365,260 potentiated electroacupuncture analgesia in Wistar rats but not in audiogenic epileptic rats.

Cholecystokinin octapeptide (CCK-8) has been shown to be a neuropeptide with potent anti-opioid activity. Previous studies have shown that central administration of nanogram dose of CCK-8 totally abolished morphine analgesia in the rat, an effect mediated by CCK-B receptor in central nervous system. In the present study CCK-B antagonist L-365,260 was injected intracerebroventricularly (icy) to Wistar rats to see its effect on the analgesic effect induced by electroacupuncture (EA) stimulation. A marked potentiation of EA-induced analgesia was observed. The degree of potentiation depends on the frequency of EA used, with a rank order of 100 Hz > 15 Hz=2/15 Hz> > 2Hz. in a strain of rat with acoustically evoked epileptic seizure (P77PMC rats), an extra-ordinarily strong analgesic effect was produced in response to 100 Hz EA stimulation, which was similar to that in Wistar rats pre-treated with L-365,260. However, L-365,260 was not effective in potentiating EA analgesia in P77PMC rats. The results suggest t     

神经科学的过去和未来

神经科学(Neuroscience)一词开始出现于20世纪60年代,泛指与神经系统的结构和功能有关的知识和研究,也称“脑科学”。人类对脑的了解落后于对其他器官的了解,这主要是由于神经系统、尤其是神经系统高级中枢——脑的结构     

γ-氨基丁酸合成和释放抑制剂3-巯基丙酸对电针和吗啡镇痛的作用

1-氨基丁酸(GABA)是脑内主要的抑制性神经介质之一,参与多种生理功能的调节。近年来有不少资料证明GABA也与吗佛镇痛有关。但在针刺镇痛中的作用,以往研究很少。本文以3-巯基丙酸(3-Mercap-toproplonic acid,3-MP)作主要工具药进     

大鼠脊髓中的强啡肽B参与电针镇痛

强啡肽B(Dynorphin B,或称Rimorphin)是新近发现的由十三个氨基酸组成的阿片样肽,存在于大鼠的脑、垂体和脊髓中。本工作将抗强啡肽B抗血清分别注射到大鼠的侧脑室或脊髓腰膨大蛛网膜下腔,观察其是否影响电针镇痛。     

脑内阿片肽在去甲肾上腺素中枢降压效应中的作用

本文分析大鼠侧脑室注射(icv)去甲肾上腺素(NE)和α受体激动剂可乐宁的心血管效应与脑内几种阿片肽的关系。实验用颈总动脉插管的麻醉动物完成。 一、侧脑室注射NE和可乐宁的降压效应及α阻断剂的预防作用:大鼠icv 10μg NE,引起降压和心率减慢反应,70分钟内血压与心率的最大降低百分数分别为-26±5     

ANTIBODY MICROINJECTION A NEW APPROACH FOR STUDYING THE FUNCTIONS OF NEUROPEPTIDES

roiiowing the rapid progress iri molecular bio logy, we are moving from the period of "one peptide a year" to a period of "one peptide a day". To cope with the rapid emergence of novel biologically active peptides, new techniques are needed to clarify their physiological functions. Evidence is presented here to show that antibody microinjection is a rapid and reliable method to evaluate the functions of endogenously released peptides. It is especially useful when no specific receptor antagonist is yet avaiilable for pharmacological study.     

中枢微量注射抗体法——一种鉴别神经肽生理功能的新方法

复杂的生命活动是由许多生物活性物质进行调节的,在神经系统中发挥调节作用的肽类物质称为神经调节肽(简称神经肽)。它们是由几个或几十个氨基酸组成的,大多具有很强的生物活性。例如1975年从猪脑中提取出一种作用类似于吗啡的肽,由5个氨基酸组成,称为脑啡肽。十年来又陆续发现了20余种作用相似的肽,统称为阿片肽,包括17个氨基酸的强啡肽和31个氨基酸的β-内啡肽等。它们的共同点是,都要通过吗啡受体起作用,因此其作用都可被吗啡受体阻断剂(纳洛酮)     

P77PMC大鼠脑内孤啡肽含量低于Wistar大鼠

孤啡肽（ｏｒｐｈａｎｉｎＦＱ,ＯＦＱ）是１９９５年底发现的孤儿阿片受体样受体的内源性配体［１］,其结构和细胞内信号转导通路方面类似于阿片肽但功能在脑内则与阿片肽相反。ＯＦＱ作为一种新的抗阿片样物质广泛存在于中枢神经系统,原位杂交［２］和Ｎｏｒｔｈｅｒ...