论传统文化对中医证理论形成的影响

证是古人在无数实践经验的基础上，基于外现的相关生命现象，从整体上把握人体内外各部的联系，对收集的各种病象进行逻辑推理而产生的一种整体功能关系失调的病理模型。其始终以中华民族优秀文化为深厚底蕴，蕴含着文学、艺术、历史、地理、天文、农学等多学科知识，呈现出一种极其复杂的文化生态，因此其形成必然受到中国传统文化及其思维方式的熏染。     

分阶段辨证治疗肠易激综合征30例

肠易激综合征（IBS）是与排便有关的临床症候群,主要表现为腹痛、腹胀、便秘、泄泻,或便秘、泄泻交替等.本病是一种十分常见的肠功能失调性疾病,其病因和发病机制至今尚未完全明确,为探求中医对本病的治疗方法,笔者选择2000年1月-2004年6月间门诊患者30例为治疗观察对象,分3个阶段组用中医五脏整体病理观进行辨证治疗取得一定效果.     

对中医证本质研究的反思

证是中医辨证论治的精髓,是理法方药一脉相承的桥梁和关键.近几十年来,证本质研究一直是中医现代化研究最主要的攻关对象.     

肺透明膜病

肺透明膜病是由于缺乏肺表面活性物质而导致的急性呼吸窘迫，故又称之为呼吸窘迫综合征。多发生于早产儿。其主要表现为生后不久出现呼吸困难，并呈进行性加重，伴有胸片的特异性改变。其发病率与胎龄成反比：24周者高达80％、28周者为70％、32周者为25％、36周者仅为5％。     

重度烧伤患者的观察及护理

重度烧伤患者的病程长．病情严重．变化多而急骤，治疗较繁杂。在治疗护理的过程中要求护理人员应具有敏锐的观察力及良好的护理能力，它是烧伤救治工作中极为重要的环节，是抢救成功过程必不可少的因素。现将我科2002年1月～2003年1月救治成功的45例重度烧伤患者的护理经验总结如下。     

脉冲激光治疗血管性疾病所致瘢痕形成的原因分析

我们科室从1999年3月至2001年8月应用固体脉冲激光仪治疗皮肤血管性疾病482例患者，除16例患者治疗部位形成瘢痕外，其余均取得满意的疗效，现将16例患者瘢痕形成的原因分析如下。     

后房型人工晶体手术前后的房角镜观察

后房型人工晶体手术前后的房角镜观察南京军区福州总院杨丽霞，高建华据文献报导，后房型人工晶体植入术后可发生房角结构的改变，如虹膜周边前粘连、色素层受损、房角色素沉着等，甚至引起继发性青光眼。我们从１９９４年２月始对后房型人工晶体植入术的３６例病人进行了...     

Adipose tissues differentiated by adipose-derived stem cells harvested from transgenic mice

Objective: To induce adipocyte differentiation in vitro by adipose-derived stromal cells (ASCs) harvested from transgenic mice with green fluorescent protein (GFP) and assess the possibility of constructing adipose tissues via attachment of ASCs to typeⅡcollagen scaffolds. Methods: Inguinal fat pads from GFP transgenic mice were digested by enzymes for isolation of ASCs (primary culture). After expansion to three passages of ASCs, the cells were incubated in an adipogenic medium for two weeks, and the adipocyte differentiation by ASCs in vitro was assessed by morphological observation and Oil Red O staining. Then they were attached to collagen scaffolds and co-cultured for 12 hours, followed by hypodermic implantation to the dorsal skin of nude mice for 2 months. The newly-formed tissues were detected by HE staining. Results: The cultured primary stem cells were fibroblast-like and showed active proliferation. After being incubated in an adipocyte differentiation medium, the lipid droplets in the cytoplasm accumulated gradually and finally developed into mature adipocytes, which showed positive in Oil Red O staining. A 0. 5-cm3 new tissue clot was found under the dorsal skin of the nude mice and it was confirmed as mature adipose tissues by fluorescent observation and HE staining. Conclusions: ASCs can successfully differentiate adipose tissues into mature adipocytes, which exhibit an adipocyte-like morphology and express as intracytoplasmic lipid droplets. It is an efficient model of adipose tissues engineered with ASCs and type I collagen scaffolds.