Enzyme activity of induced auxotrophic mutants of El Tor Vibrio cholerae

Vibrio cholerae induced auxotrophic variants nonfermenting several carbohydrates simultaneously have been obtained for the first time. Eltor vibrio clones have been selected, referred to Heuberg's groups V and VI by their enzymic activities. The findings of the present study evidence that, when isolating Eltor vibrio strains negative towards differential carbohydrates, in case of a positive agglutination test, the incubation period should be prolonged to 3 days. The principal differential diagnostic test for this species, when cholera is bacteriologically diagnosed, should be a positive volumic agglutination test with species- and variant-specific sera, irrespective of Heuberg's group.     

Normobaric intermittent hypoxia and functional state of the erythrocyte pool]

Functional state of the pool of erythrocytes was evaluated in ten essentially healthy male subjects before, during and in 2 months after a series of 15 exposures to normobaric intermittent hypoxia (NIH). The erythrocyte pool dynamics, hemoglobin content, low and highly resistive fractions of erythrocytes were analyzed using a modified acidic histogram technique. It was demonstrated that the erythrocyte pool was either in the state of destruction (concurrent to the NIH exposure) or ensuing persistent improvement of the functional characteristics under study.     

Emission-computed tomography in the diagnosis of kidney tumors and cysts]

Emission computed tomography (ECT) was used to examine 48 patients with suspected renal lesions. Sectional images of renal cysts diagnosed in 14 of them were characterized by well-defined outlines surrounded by uniformly declining radiopharmaceutical accumulations. Renal tumors were detected in 25 patients. ECT pattern of the tumor had obscure borders suggesting the tumor invasion into the renal tissue. Accumulation of the radiopharmaceutical round the tumor was less pronounced than in functioning renal tissue. According to ECT findings, 8 subjects were free of urological pathology. It is concluded that ECT is a promising diagnostic tool in urology as it permits sectional visualization of renal neoplasms structure, is highly sensitive, informative and noninvasive. It can identify mass lesions of the kidneys and warrants exhaustive information on functional performance of the renal parenchyma.     

Structural changes in dendritic spines of the pyramidal neurons of layer III of the rat sensory-motor cortex during remote postischemic period

The combination of Golgi method with the lipid phosphorilate mark Dil (1,1'-dioctadecyl-3,3,3 cents, 3 cents-tetramethyl-indocarbocyanine perchlorate) and confocal laser scanning microscope for demonstration of the structure of dendritic tree and dendritic spines enables exact determination of spatial organization of small dendrites and their spines at significant distances in norm and especially in postischemic period. The regularities of dendritic spine reorganization in layer III pyramidal neurons of the cerebral sensory-motor cortex during 9 months after short-term (10 min) total brain ischemia were established.     

Physicochemical and antimicrobial properties of chlorine-containing compositions ciaref and DP-1

The paper presents the results of the physicochemical properties and corrosive activity of the new chlorine-containing compositions Ciaref and DP-1. Various microbiological, biochemical, and biophysical studies were conducted to examine different aspects of the mechanism of action of Ciaref and DP-1 on vegetative (Francisella tularensis) and sporal (Bacillus turingiensis) microorganisms. Suspension, test-surface irrigation, and aerosol techniques were employed under close industrial conditions to study the disinfecting properties of the agents in bacterial and sporal contamination.