Down-link OFDRMA communications

Wireless Networks - Orthogonal frequency-division multiplexing with random multiple access (OFDRMA) is discussed for down-link communications, whereby a single base-station transmits information...     

Efficient Incorporation of Clickable Unnatural Amino Acids Enables Rapid and Biocompatible Labeling of Proteins in Vitro and in Bacteria

Site-specific incorporation of unnatural amino acids (uAAs) bearing a bioorthogonal group has enabled the attachment – typically at a single site or at a few sites per protein – of chemical groups at precise locations for protein and biomaterial labeling, conjugation, and functionalization. Herein, we report the evolution of chromosomal Methanocaldococcus jannaschii tyrosyl-tRNA synthetase (aaRS) for the alkyne-bearing uAA, 4-propargyloxy-l -phenylalanine (pPR), with ∼30-fold increased production of green fluorescent protein containing three instances of pPR compared with a previously described M. jannaschii-derived aaRS for pPR, when expressed from a single chromosomal copy. We show that when expressed from multicopy plasmids, the evolved aaRSs enable the production – using a genomically recoded Escherichia coli and the non-recoded BL21 E. coli strain – of elastin-like polypeptides (ELPs) containing multiple pPR residues in high yields. We further show that the multisite incorporation of pPR in ELPs facilitates the rapid, robust, and nontoxic fluorescent labeling of these proteins in bacteria. The evolved variants described in this work can be used to produce a variety of protein and biomaterial conjugates and to create efficient minimal tags for protein labeling.     

Direct positioning of stationary targets using MIMO radar

MIMO radar is a new concept that has recently been proposed as an extension to the multi-static radar systems. We apply the direct position determination (DPD) approach to MIMO radar and obtain maximum likelihood algorithms for a location estimation of a stationary target. It is shown that under low signal to noise ratio DPD improves the estimation accuracy with respect to the traditional location methods.     

A three-person perspective on transference.

Different approaches to psychoanalysis may be classed according to the number of persons that they assume to be necessary in order to adequately describe mental life. One-person approaches assume the basic autonomy of the individual to act as a subject in her world, while 2-person approaches assume the irreducibility of object and subject, their essential complementarity and their mutual affirmation as subjects. Three-person approaches, on which the present article focuses, argue that the subject can fully develop only by creating enough space for herself among other persons who compete for subject positions. Such space is created in relation to (at least) 2 other subjects, hence the system of 3 persons. In the 3-person perspective, the position of the subject is defined as First, and the Second is defined by the one with whom the subject identifies and in whom she mirrors herself through cycles of projection and introjection. The position of the Third involves the personification of the cultural matrix and, especially, the way language informs our ability to relate to each other. This is best represented in the universal system of personal pronouns: the subject takes the position of the 1st person (I), the Second takes the position of the 2nd person (You), and the Third is posited as the 3rd person (she or he and it). In psychoanalytic therapy, the patient takes the position of the Subject, the First. Transference is construed as the superposition of both the 2nd and the 3rd persons upon the figure of the analyst, a mental process that (re)creates the necessary conditions for the development of subjecthood. As a result, in the discourse of transference, the position of the analyst keeps fluctuating between the 2nd and 3rd positions: When one takes place on the actual level, the other acts in the background, and vice versa. The therapeutic consequences of this view are discussed; for example, transference love is construed as a process in which the 2nd person struggles to dominate the positioning of the analyst. Other transferential configurations are also discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Hydrothermal degradation of adsorbed sulfur mustard on activated carbon

Thermal and hydrothermal degradations of adsorbed sulfur mustard (HD) on activated carbon particles from a chemical protective over-garment were studied. Carbon loaded with 5 wt.% HD was heated in a closed reactor at temperatures up to 160 °C for 0.5–6 h and analyzed by solid-state ¹³C MAS NMR. On dry carbon at room temperature, HD was stable for months. On a thoroughly pre-wetted carbon, adsorbed HD partially degraded to thiodiglycol (TDG) and TDG-sulfoxide (TDG-SO) within 2–3 months. Heating dry HD-loaded carbon to 160 °C caused partial degradation within 4 h to 1,4-thioxane, along with 1,4-dithiane and vinyl sulfides. Complete degradation within 2.5 h to the same products occurred upon hydrothermal treatment of the HD-loaded carbon, using a water/carbon ratio of 0.3:1. With higher water/carbon ratios of 0.6:1–5:1 at temperatures of 120 °C and above, adsorbed HD hydrolyzed rapidly within 0.5 h. The latter reaction led to the formation of TDG concurrent with either thioxane (at 160 °C) or TDG-SO and TDG-dimer (at 120 °C). The mechanisms of the observed degradation processes are discussed.     

Easy on that trigger dad: a study of long term family photo retrieval

We examine the effects of new technologies for digital photography on people’s longer term storage and access to collections of personal photos. We report an empirical study of parents’ ability to retrieve photos related to salient family events from more than a year ago. Performance was relatively poor with people failing to find almost 40% of pictures. We analyze participants’ organizational and access strategies to identify reasons for this poor performance. Possible reasons for retrieval failure include: storing too many pictures, rudimentary organization, use of multiple storage systems, failure to maintain collections and participants’ false beliefs about their ability to access photos. We conclude by exploring the technical and theoretical implications of these findings.     

Fast GL(n)-Invariant Framework for Tensors Regularization

We propose a novel framework for regularization of symmetric positive-definite (SPD) tensors (e.g., diffusion tensors). This framework is based on a local differential geometric approach. The manifold of symmetric positive-definite (SPD) matrices, P _n , is parameterized via the Iwasawa coordinate system. In this framework distances on P _n are measured in terms of a natural GL(n)-invariant metric. Via the mathematical concept of fiber bundles, we describe the tensor-valued image as a section where the metric over the section is induced by the metric over P _n . Then, a functional over the sections accompanied by a suitable data fitting term is defined. The variation of this functional with respect to the Iwasawa coordinates leads to a set of \frac12n(n+1)\frac{1}{2}n(n+1) coupled equations of motion. By means of the gradient descent method, these equations of motion define a Beltrami flow over P _n . It turns out that the local coordinate approach via the Iwasawa coordinate system results in very simple numerics that leads to fast convergence of the algorithm. Regularization results as well as results of fibers tractography for DTI are presented.     

Triplet State Interrogation in Supersonic Beams by Surface Electron Ejection

Recently, we have developed an experimental method for the detection of triplet states generated by laser excitation in supersonic beams. It is based on electron ejection from low work-function surfaces by metastable triplet states. We have detected both directly laser-excited triplets and triplets generated via intersystem crossing from laser-excited singlet states. Here, we review the applications of this method and discuss its mechanism. By comparing the laser-induced fluorescence (LIF) spectrum and Surface Electron Ejection by Laser-Excited Metastables (SEELEM), we have measured relative triplet formation quantum yields for several aromatic compounds. By utilizing a detector mounted on a translational stage, we could vary the distance between the pulsed laser excitation and the detector and measure the decay rates of triplets in molecular beams. The major advantage of the method is in extending the measurement of triplet lifetimes to the ∼ 1-ms range. The combination of LIF, SEELEM, and fluorescence quantum yields enabled us to discriminate between intersystem crossing and internal conversion in isoquinoline. SEELEM is now being utilized in studying the spectroscopy and the dynamics of directly laser-excited triplet states. Although the oscillator strength of the lowest triplet state of pyrazine is about 10⁻⁸, we have measured the spectrum and the decay rates of its various vibronic levels. Our results support the notion that surface Penning ionization is the mechanism of SEELEM. The detector is insensitive to vibrational energy (thus enabling the distinction between intersystem crossing and internal conversion). The detection sensitivity of triplets rises with the excess electronic energy and with the lowering of the surface work-function.     

Improving Social Recommender Systems

Recommender systems play a significant role in reducing information overload for people visiting online sites, but their accuracy could be improved by using data from online social networks and electronic communication tools.