首页 | 官方网站   微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   493篇
  免费   15篇
  国内免费   1篇
工业技术   509篇
  2023年   3篇
  2021年   5篇
  2020年   3篇
  2019年   8篇
  2018年   2篇
  2017年   2篇
  2016年   10篇
  2015年   6篇
  2014年   10篇
  2013年   12篇
  2012年   17篇
  2011年   14篇
  2010年   19篇
  2009年   13篇
  2008年   20篇
  2007年   15篇
  2006年   21篇
  2005年   12篇
  2004年   18篇
  2003年   9篇
  2002年   11篇
  2001年   7篇
  2000年   9篇
  1999年   14篇
  1998年   69篇
  1997年   35篇
  1996年   23篇
  1995年   15篇
  1994年   11篇
  1993年   14篇
  1992年   8篇
  1991年   3篇
  1990年   4篇
  1989年   6篇
  1988年   6篇
  1987年   4篇
  1986年   2篇
  1985年   2篇
  1984年   3篇
  1982年   3篇
  1981年   4篇
  1980年   6篇
  1979年   3篇
  1978年   5篇
  1977年   6篇
  1976年   3篇
  1974年   5篇
  1971年   3篇
  1961年   1篇
  1960年   2篇
排序方式: 共有509条查询结果,搜索用时 15 毫秒
1.
Pactamycin is an antibiotic produced by Streptomyces pactum with antitumor and antimalarial properties. Pactamycin has a unique aminocyclitol core that is decorated with 3-aminoacetophenone, 6-methylsaliciate, and an N,N-dimethylcarbamoyl group. Herein, we show that the adenylation enzyme PctU activates 3-aminobenzoic acid (3ABA) with adenosine triphosphate and ligates it to the holo form of the discrete acyl carrier protein PctK to yield 3ABA-PctK. Then, 3ABA-PctK is N-glycosylated with uridine diphosphate-N-acetyl-d -glucosamine (UDP-GlcNAc) by the glycosyltransferase PctL to yield GlcNAc-3ABA-PctK. Because 3ABA is known to be a precursor of the 3-aminoacetophenone moiety, PctU appears to be a gatekeeper that selects the appropriate 3-aminobenzoate starter unit. Overall, we propose that acyl carrier protein-bound glycosylated 3ABA derivatives are biosynthetic intermediates of pactamycin biosynthesis.  相似文献   
2.
The high penetration of variable sources of renewable power generation will lead to operational difficulties in supply/demand balancing in the entire power system. The mass deployment of electric vehicles (EVs) and plug‐in hybrid vehicles (PHEVs) will also cause significant changes in electricity demand. Therefore, controlling and managing the charging time of EVs/PHEVs are effective approaches that are imperative for improving balancing in power system operation. We assumed travel patterns for EVs in a model of the future Tokyo power system and analyzed the power system loads, including the charging load of the EVs, under several charging control scenarios. We verified that charging time controls are substantially effective for reducing the fuel costs in the power system. Further, we found that load leveling under a multicar charging management scenario gave the best results in terms of the fuel costs in all cases.  相似文献   
3.
The unique five‐membered aminocyclitol core of the antitumor antibiotic pactamycin originates from d ‐glucose, so unprecedented enzymatic modifications of the sugar intermediate are involved in the biosynthesis. However, the order of the modification reactions remains elusive. Herein, we examined the timing of introduction of an amino group into certain sugar‐derived intermediates by using recombinant enzymes that were encoded in the pactamycin biosynthesis gene cluster. We found that the NAD+‐dependent alcohol dehydrogenase PctP and pyridoxal 5′‐phosphate dependent aminotransferase PctC converted N‐acetyl‐d ‐glucosaminyl‐3‐aminoacetophonone into 3′‐amino‐3′‐deoxy‐N‐acetyl‐d ‐glucosaminyl‐3‐aminoacetophenone. Further, N‐acetyl‐d ‐glucosaminyl‐3‐aminophenyl‐β‐oxopropanoic acid ethyl ester was converted into the corresponding 3′‐amino derivative. However, PctP did not oxidize most of the tested d ‐glucose derivatives, including UDP‐GlcNAc. Thus, modification of the GlcNAc moiety in pactamycin biosynthesis appears to occur after the glycosylation of aniline derivatives.  相似文献   
4.
Metallurgical and Materials Transactions B - A two-step plasma arc melting process, comprising a first step under Ar-30 pct H2 gas flow and the second step under Ar gas flow, has been...  相似文献   
5.
6.
Temporal segmentation of successive actions in a long-term video sequence has been a long-standing problem in computer vision. In this paper, we exploit a novel learning-based framework. Given a video sequence, only a few characteristic frames are selected by the proposed selection algorithm, and then the likelihood to trained models is calculated in a pair-wise way, and finally segmentation is obtained as the optimal model sequence to realize the maximum likelihood. The average accuracy on IXMAS dataset reached to 80.5% at frame level, using only 16.5% of all frames in computation time of 1.57 s per video which has 1160 frames on the average.  相似文献   
7.
Innovative sensing systems based on THz electromagnetic waves have been attracting a great deal of attention. Although many THz detectors have been developed over the years, it is currently difficult to manufacture low-cost THz sensing/imaging devices. In the present study, we propose to use organic field-effect transistors (OFETs) and small potential fluctuation against the carriers within them (N. Ohashi, H. Tomii, R. Matsubara, M. Sakai, K. Kudo, M. Nakamura, Appl. Phys. Lett. 91 (2007) 162105). We use THz time-domain spectroscopy for OFETs in which the carrier density in the pentacene active layer is modulated by the gate bias. We found evidence that the accumulated free holes in pentacene films can be excited by THz photons to overcome the surrounding barriers in the fluctuating potential. The Drude–Lorentz model could not account for the shape of the absorption spectra, which suggests that the holes are weakly restricted by the potential fluctuation. The integrated absorption intensity was proportional to the transfer characteristics of the OFETs. The present findings represent an important step toward developing a new class of THz-wave sensors.  相似文献   
8.
To accurately recognize human actions in less computational time is one important aspect for practical usage. This paper presents an efficient framework for recognizing actions by a RGB-D camera. The novel action patterns in the framework are extracted via computing position offset of 3D skeletal body joints locally in the temporal extent of video. Action recognition is then performed by assembling these offset vectors using a bag-of-words framework and also by considering the spatial independence of body joints. We conducted extensive experiments on two benchmarking datasets: UCF dataset and MSRC-12 dataset, to demonstrate the effectiveness of the proposed framework. Experimental results suggest that the proposed framework 1) is very fast to extract action patterns and very simple in implementation; and 2) can achieve a comparable or a better performance in recognition accuracy compared with the state-of-the-art approaches.  相似文献   
9.
10.
Mutational analysis of the pyridoxal 5′‐phosphate (PLP)‐dependent enzyme PctV was carried out to elucidate the multi‐step reaction mechanism for the formation of 3‐aminobenzoate (3‐ABA) from 3‐dehydroshikimate (3‐DSA). Introduction of mutation K276R led to the accumulation of a quinonoid intermediate with an absorption maximum at 580 nm after the reaction of pyridoxamine 5′‐phosphate (PMP) with 3‐DSA. The chemical structure of this intermediate was supported by X‐ray crystallographic analysis of the complex formed between the K276R mutant and the quinonoid intermediate. These results clearly show that a quinonoid intermediate is involved in the formation of 3‐ABA. They also indicate that Lys276 (in the active site of PctV) plays multiple roles, including acid/base catalysis during the dehydration reaction of the quinonoid intermediate.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号