全文获取类型
收费全文 | 4914篇 |
免费 | 76篇 |
国内免费 | 9篇 |
学科分类
工业技术 | 4999篇 |
出版年
2023年 | 15篇 |
2021年 | 57篇 |
2020年 | 31篇 |
2019年 | 29篇 |
2018年 | 48篇 |
2017年 | 51篇 |
2016年 | 87篇 |
2015年 | 60篇 |
2014年 | 82篇 |
2013年 | 196篇 |
2012年 | 167篇 |
2011年 | 196篇 |
2010年 | 128篇 |
2009年 | 176篇 |
2008年 | 179篇 |
2007年 | 139篇 |
2006年 | 136篇 |
2005年 | 134篇 |
2004年 | 100篇 |
2003年 | 116篇 |
2002年 | 115篇 |
2001年 | 110篇 |
2000年 | 91篇 |
1999年 | 138篇 |
1998年 | 499篇 |
1997年 | 293篇 |
1996年 | 253篇 |
1995年 | 151篇 |
1994年 | 143篇 |
1993年 | 130篇 |
1992年 | 66篇 |
1991年 | 73篇 |
1990年 | 56篇 |
1989年 | 46篇 |
1988年 | 56篇 |
1987年 | 48篇 |
1986年 | 49篇 |
1985年 | 47篇 |
1984年 | 58篇 |
1983年 | 54篇 |
1982年 | 44篇 |
1981年 | 42篇 |
1980年 | 51篇 |
1979年 | 34篇 |
1978年 | 31篇 |
1977年 | 30篇 |
1976年 | 51篇 |
1975年 | 15篇 |
1974年 | 25篇 |
1973年 | 13篇 |
排序方式: 共有4999条查询结果,搜索用时 120 毫秒
1.
Risako Kobayashi Hiroshi Inaba Kazunori Matsuura 《International journal of molecular sciences》2021,22(9)
Recent progress in the de novo design of self-assembling peptides has enabled the construction of peptide-based viral capsids. Previously, we demonstrated that 24-mer β-annulus peptides from tomato bushy stunt virus spontaneously self-assemble into an artificial viral capsid. Here we propose to use the artificial viral capsid through the self-assembly of β-annulus peptide as a simple model to analyze the effect of molecular crowding environment on the formation process of viral capsid. Artificial viral capsids formed by co-assembly of fluorescent-labelled and unmodified β-annulus peptides in dilute aqueous solutions and under molecular crowding conditions were analyzed using fluorescence correlation spectroscopy (FCS). The apparent particle size and the dissociation constant (Kd) of the assemblies decreased with increasing concentration of the molecular crowding agent, i.e., polyethylene glycol (PEG). This is the first successful in situ analysis of self-assembling process of artificial viral capsid under molecular crowding conditions. 相似文献
2.
Abidur Rahman Makoto Kobayashi Kotaro Sugimoto Yuta Endo Manabu Kojima Shigenori Furukawa Takafumi Watanabe Shu Soeda Yuko Hashimoto Keiya Fujimori Hideki Chiba 《International journal of molecular sciences》2021,22(7)
Background: Within the claudin (CLDN) family, CLDN12 mRNA expression is altered in various types of cancer, but its clinicopathological relevance has yet to be established due to the absence of specific antibodies (Abs) with broad applications. Methods: We generated a monoclonal Ab (mAb) against human/mouse CLDN12 and verified its specificity. By performing immunohistochemical staining and semiquantification, we evaluated the relationship between CLDN12 expression and clinicopathological parameters in tissues from 138 cases of cervical cancer. Results: Western blot and immunohistochemical analyses revealed that the established mAb selectively recognized the CLDN12 protein. Twenty six of the 138 cases (18.8%) showed low CLDN12 expression, and the disease-specific survival (DSS) and recurrence-free survival rates were significantly decreased compared with those in the high CLDN12 expression group. We also demonstrated, via univariable and multivariable analyses, that the low CLDN12 expression represents a significant prognostic factor for the DSS of cervical cancer patients (HR 3.412, p = 0.002 and HR 2.615, p = 0.029, respectively). Conclusions: It can be concluded that a reduced CLDN12 expression predicts a poor outcome for cervical cancer. The novel anti-CLDN12 mAb could be a valuable tool to evaluate the biological relevance of the CLDN12 expression in diverse cancer types and other diseases. 相似文献
3.
Sunsanee Udomrati Thidarat Pantoa Shoichi Gohtani Mitsutoshi Nakajima Kunihiko Uemura Isao Kobayashi 《International Journal of Food Science & Technology》2020,55(4):1437-1447
Water-soluble soybean polysaccharide (SSPS) is a naturally occurring emulsifier. SSPS was used as the sole emulsifier to stabilize an oil-in-water (O/W) emulsion. The effects were investigated of different SSPS concentrations (3–20% (w/w)) on the lipid digestibility, rheological properties and stability of O/W emulsions during in vitro digestion model. The droplet size of the emulsions tended to increase during the oral phase because the emulsions were unstable and droplets coalesced, except with a SSPS concentration of 20% (w/w). The presence of SSPS markedly reduced the free fatty acid (FFA) content after its stabilized O/W emulsion passed through in vitro gastrointestinal digestion. The amount of FFA significantly decreased as the concentration of SSPS increased due to SSPS stabilization film on oil droplet surface and high viscous system. SSPS may be an attractive alternative ingredient to control the lipid digestibility of emulsions for various food products. 相似文献
4.
Hiromi Nakano 《Advanced Powder Technology》2019,30(10):2003-2013
In the Li2O–M2O5–TiO2 (M = Nb or Ta) system, Li1+x-yM1-x-3yTix+4yO3 (LMT) forms with a superstructure known as the M-phase, in which the periodical intergrowth layers are inserted in a matrix having a trigonal structure of LiMO3. We have been investigating this unique material focusing on its crystal structure, photoluminescence, and electrical properties.First, the formation area of the superstructure was compared between Nb- and Ta- systems and the difference was accurately analyzed.Second, to apply this unique material as a host material of phosphor, we synthesized new phosphors with various emission colors. Red phosphor, having a high PL intensity with an internal quantum efficiency of 98%, was successfully synthesized and used as a host material of the solid solution for the Ta system. Its high value was the result of the large centroid-to-cation distance of the Eu3+ position in the [(Li, Eu)O12] polyhedral.Further still, toward application of the unique qualities of an electro-ceramic, we successfully fabricated oriented balk ceramics for the Nb system by slip casting in a strong magnetic field of 12 T. As a result, anisotropic electric properties were found along the c-axis, which were caused by the superstructure. We first clarified the mechanism showing that the anisotropic Qf value was due to anisotropic electron conductivity and anisotropic bonding strength within the superstructure. 相似文献
5.
Hiromi Eba Yuki Masuzoe Toru Sugihara Hayao Yagi Tian Liu 《International Journal of Hydrogen Energy》2021,46(18):10642-10652
Ammonia generation was studied in the reaction between water and nitrogen-containing iron at 323 K and atmospheric pressure. Similar to metallic Fe, the interstitial compound Fe3N reduced water through Fe oxidation to produce hydrogen gas, while the N combined with atomic hydrogen to produce ammonia as a byproduct. The addition of carbon dioxide to this system accelerated the reaction with concomitant consumption of carbon dioxide. The promoted ammonia production upon addition of carbon dioxide can be attributed to the generation of atomic hydrogen from the redox reaction of carbonic acid and Fe, as well as removal of used Fe from the reaction system through the formation of a soluble carbonato complex. When carbonate was added to the reaction system, the production rates of ammonia and hydrogen increased further. The results here confirmed that ammonia can be synthesized from iron nitride under mild conditions by utilizing carbon dioxide. 相似文献
6.
7.
Kento Ishii Chika Matsunaga Kiyoshi Kobayashi Adam J. Stevenson Caroline Tardivat Tetsuo Uchikoshi 《Journal of the European Ceramic Society》2021,41(4):2709-2715
The Ba0.5Sr0.5Co0.8Fe0.2O3-δ (BSCF)-based multi-layered oxygen separation membrane was fabricated by the sequential electrophoretic deposition (EPD) process. A thin porous/dense bi-layer of BSCF was formed on a thick porous support of BSCF. The porous support prepared by a sacrificial template method using BSCF powder mixed with wheat starch (30 wt%) as a pore-forming agent, followed by uniaxial pressing and low-temperature sintering, was directly used as an EPD electrode. A thin BSCF layer was first formed on the porous support, and then a thin BSCF + PMMA (polymethyl methacrylate) layer was sequentially formed on the thin BSCF layer using a bimodal suspension of BSCF and PMMA. A 30-μm thin porous/dense bi-layer of BSCF of which the total thickness was obtained by optimizing the processes of EPD and subsequent co-sintering. The oxygen separation performance of 3.7 ml (STP) min?1 cm?2 at 860 °C was achieved for the BSCF-based multi-layered oxygen separation membrane. 相似文献
8.
9.
Consideration of multi‐coil type magnetization system for magnetic particle testing of omnidirectional crack in all surfaces of 3D shape test object 下载免费PDF全文
Katsuhiro Fukuoka Masaki Kobayashi Tomohiro Ozaki Yoshiro Oikawa 《Electrical Engineering in Japan》2018,204(4):36-42
Mechanical parts, plants, and cross‐linkages inspected with magnetic‐particle testing (MT) are typically complex 3D shapes. In complex 3D shape portions, because a magnetizer often cannot be configured to inspection portions and the test object cannot be appropriately magnetized, there is a possibility of overlooking a crack in such an instance. Thus, MT system development that was successfully able to detect omnidirectional cracks in 3D shape portions was considered in this study's trials. Two multi‐coil type magnetizers were hence arranged face‐to‐face, and the magnetization of omnidirectional scenarios for all surfaces of 3D shape test object was evaluated. 相似文献