Application of the novel Droplet digital PCR technology for identification of meat species

The authenticity and traceability of meat products are issues of primary importance to ensure food safety. Unfortunately, food adulteration (e.g. the addition of inexpensive cuts to minced meat products) and mislabelling (e.g. the inclusion of meat from species other than those declared) happens frequently worldwide. The aim of this study was to apply a droplet digital PCR assay for the detection and quantification (copies μL⁻¹) of the beef, pork, horse, sheep, chicken and turkey in meat products. The analysis conducted on commercial meat showed the presence of traces of DNA from other animal species than those declared. We show that the method is highly sensitive, specific and accurate (accuracy = 100%). This method could be adopted by competent food safety authorities to verify compliance with the labelling of meat products and to ensure quality and safety throughout the meat supply chain, from primary production to consumption.     

Autochthonous white grape pomaces as bioactive source for functional jams

Seeds and skins from grape pomaces of Pecorello and Mantonico cv underwent extraction (ultrasound–assisted or maceration), in order to obtain added-value ingredients for the production of a functional pear jam. The antioxidant features of the extracts were tested by in vitro colorimetric assays. Among seeds, Mantonico by maceration (MSC) showed the best results, as well as Mantonico by ultrasound-assisted extraction (MBs) among skin extracts. The selected extracts were fully characterised by NMR and MS techniques, confirming the presence of many polyphenols, flavonoids and tannins among others. Pectin was then derivatised by the grafting procedure with the active molecules of MBs and MSC. The latter produced the best antioxidant polymer also without toxicity evaluated using Caco-2 cells and was used for the jam preparation. The functional pear jam showed improved antioxidant performances in comparison with its non-functional counterparts as well as its maintenance over time (15 days).     

Phosphates and ammonia recovery from secondary effluents by selective ion exchange with production of a slow-release fertilizer

A process for the reduction of eutrophic potential in urban secondary effluents. which comprises the selective exchange of phosphates on a weak anion resin and of ammonia on clinoptilolite, is described. Sodium chloride is used to regenerate both resins, with some Ca(OH)₂ added for clinoptilolite. By adding Mg(OH)₂, eventually precipitated with lime from sea water, the premium quality, slow-release fertilizer MgNH₄PO₄-6H₂O is recovered from the concentrated regeneration streams, which can then be recycled. Laboratory evaluations of the hydrolysis extent of the weak anion resin in the presence of bicarbonates showed that a steady-state is attained with resin exchange capacity reproducibly averaging about 80 mmol phosphates 1,⁻. The preliminary runs on a pilot plant for the tertiary treatment of urban sewage are also presented.     

Different Stages of Quiescence,Senescence, and Cell Stress Identified by Molecular Algorithm Based on the Expression of Ki67, RPS6, and Beta-Galactosidase Activity

During their life span, cells have two possible states: a non-cycling, quiescent state (G0) and a cycling, activated state. Cells may enter a reversible G0 state of quiescence or, alternatively, they may undergo an irreversible G0 state. The latter may be a physiological differentiation or, following a stress event, a senescent status. Discrimination among the several G0 states represents a significant investigation, since quiescence, differentiation, and senescence are progressive phenomena with intermediate transitional stages. We used the expression of Ki67, RPS6, and beta-galactosidase to identify healthy cells that progressively enter and leave quiescence through G0-entry, G0 and G0-alert states. We then evaluated how cells may enter senescence following a genotoxic stressful event. We identified an initial stress stage with the expression of beta-galactosidase and Ki67 proliferation marker. Cells may recover from stress events or become senescent passing through early and late senescence states. Discrimination between quiescence and senescence was based on the expression of RPS6, a marker of active protein synthesis that is present in senescent cells but absent in quiescent cells. Even taking into account that fixed G0 states do not exist, our molecular algorithm may represent a method for identifying turning points of G0 transitional states that continuously change.     

An <Emphasis Type="Italic">ex post</Emphasis> criticism,based on stakeholders’ preferences,of a residential sector’s energy master plan: the case study of the Sicilian region

Energy master plans are important tools for an effective and sustainable land governance. On the other hand, the stakeholder role in setting priorities for planning energy interventions is dramatically increasing, also in the light of recently issued European directives. The priorities of energy-saving measures of the Sicilian energy and environmental master plan were originally established with the application of typical economic indicators (the cost of saved energy and the cost of avoided pollutant emissions). During the prioritization process, there was a minor contribution from stakeholders who were not directly involved in the priority-setting process but were only asked to express their opinions according to the in-force regulations. Based on more active stakeholder involvement in hierarchizing a given set of actions, as required by the recently issued directives, the University of Palermo, which participated in the development of this master plan, assessed an ex post evaluation of these established economy-based priorities, by means of the application of a multi-criteria decision analysis (MCDA) tool. The resulting changed priorities, which lead to a better policy allocation of the regional budget for energy efficiency in the building sector, confirm the preeminent role played by the stakeholders in the development of energy plans, further supported by a sensitivity analysis.     

Perception of Host Plant Volatiles in Hyalesthes obsoletus: Behavior, Morphology, and Electrophysiology

The Palearctic planthopper Hyalesthes obsoletus is the natural vector of the grapevine yellow disease Bois noir. Grapevine is an occasional host plant of this polyphagous planthopper. To deepen our knowledge of the role of plant volatile organic compounds for H. obsoletus host plant searching, we carried out behavioral, morphological, and electrophysiological studies. We tested the attraction of H. obsoletus to nettle, field bindweed, hedge bindweed, chaste tree, and grapevine by using a Y-shaped olfactometer. The results showed a significant attraction of male H. obsoletus to chaste tree, and of the females to nettle. Male H. obsoletus were repelled by odor from hedge bindweed. Ultrastructural studies of the antennae showed at least two types of olfactory sensilla at the antennal pedicel: plaque organs and trichoid sensilla. Volatile organic compounds from nettle and chaste tree were collected, and the extracts were analyzed by coupling gas-chromatography to both mass-spectrometry and electroantennography. The volatile organic compounds that elicited electrophysiological responses in male and female antennae were identified. These findings are discussed with respect to behavior of H. obsoletus males and females in the field.