Trans-differentiation of human adipose-derived mesenchymal stem cells into cardiomyocyte-like cells on decellularized bovine myocardial extracellular matrix-based films

In this study, we aimed at fabricating decellularized bovine myocardial extracellular matrix-based films (dMEbF) for cardiac tissue engineering (CTE). The decellularization process was carried out utilizing four consecutive stages including hypotonic treatment, detergent treatment, enzymatic digestion and decontamination, respectively. In order to fabricate the dMEbF, dBM were digested with pepsin and gelation process was conducted. dMEbF were then crosslinked with N-hydroxysuccinimide/1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (NHS/EDC) to increase their durability. Nuclear contents of native BM and decellularized BM (dBM) tissues were determined with DNA content analysis and agarose-gel electrophoresis. Cell viability on dMEbF for 3rd, 7th, and 14th days was assessed by MTT assay. Cell attachment on dMEbF was also studied by scanning electron microscopy. Trans-differentiation capacity of human adipose-derived mesenchymal stem cells (hAMSCs) into cardiomyocyte-like cells on dMEbF were also evaluated by histochemical and immunohistochemical analyses. DNA contents for native and dBM were, respectively, found as 886.11?±?164.85 and 47.66?±?0.09?ng/mg dry weight, indicating a successful decellularization process. The results of glycosaminoglycan and hydroxyproline assay, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), performed in order to characterize the extracellular matrix (ECM) composition of native and dBM tissue, showed that the BM matrix was not damaged during the proposed method. Lastly, regarding the histological study, dMEbF not only mimics native ECM, but also induces the stem cells into cardiomyocyte-like cells phenotype which brings it the potential of use in CTE.     

A system-wide approach to minimize the operational cost of bench production in open-cast mining operations

International Journal of Coal Science & Technology - The production cycle of open-cast coal mines generally includes drilling, blasting, loading, hauling and coal preparation activities....     

Pre-compensation of servo tracking errors through data-based reference trajectory modification

This paper presents a new dynamic error compensation approach with novel data-based closed-loop tuning scheme to enhance tracking accuracy of machine tool feed-drives. Both servo dynamics and friction disturbance induced positioning errors are pre-compensated by modifying the reference trajectory. Velocity and acceleration profiles of reference trajectory are modulated to achieve perfect tracking. Reference position profile is modified based on the pre-sliding friction regime to eliminate quadrant glitches. Optimal error compensation is achieved by a digital trajectory pre-filter whose parameters are tuned automatically by making on-the-fly iterative adjustments. Effectiveness of proposed compensation approach is validated experimentally in multi-axis feed-drive systems.     

Advanced controller design based on gain and phase margin for microgrid containing PV/WTG/Fuel cell/Electrolyzer/BESS

Nowadays, with the increase in the amount of power generation related to renewable energy resources, the need for energy storage and management is raised. In this regard, the hydrogen energy plays a critical role in the development of renewable technologies. In view of the above, advanced controller design is presented in this paper to effectively perform load frequency control of islanded fuel cell microgrid based on the wind turbine, photovoltaic, fuel cell, electrolyzer, battery energy storage systems, and residential and commercial loads. The controller design is based on the determination of the controller parameters that the fuel cell microgrid system will provide the desired dynamic properties. In the proposed controller design, virtual gain and phase margin testers are added to provide the desired dynamic properties. The controller's stable parameter plane is determined with the help of the stability boundary locus method, taking into account time delay, gain, and phase margin. First, the accuracy of the stable parameter plane determined for the proposed controller design is demonstrated by means of time domain and eigenvalue analyzes. Finally, in order to show the performance of the advanced controller design and the success of the fuel cell as a backup generator, analysis studies have been carried out using actual data of solar and wind, and appropriate changes of load in studied microgrid.     

The Water-Soluble Peripheral Substituted Phthalocyanines as Corrosion Inhibitors for Copper in 0.1 N HCl: Gravimetric,Electrochemical, SEM-EDS,and Quantum Chemical Calculations

Protection of Metals and Physical Chemistry of Surfaces - Corrosion inhibition effects of water-soluble peripheral substituted cobalt, copper and zinc metallophthalocyanines (CoPc (1), CuPc (2) and...     

Enhancing stability region of time‐delayed smart power grids by non‐integer controllers

In this study, the impacts of non‐integer order controller on the stable parameter space of the microgrid (MG) frequency control system with fixed communication time delay are investigated and discussed with the help of the stability boundary locus (SBL) method. This study proposes a non‐integer order controller for the load frequency control (LFC) of the MG systems. To that end, the load frequency model of the MG is formulated and then the characteristic equations of this model are obtained. Then, with the help of this characteristic equation, the stable parameter space of the non‐integer controller is determined with regard to different time delay (τ ) and fractional integral order values (α ) using the SBL method. In order to show the accuracy of the obtained stable parameter space, time domain and generalized modified Mikhailov (GMM) criterion studies are carried out for different values of (τ ) and (α ). According to the results obtained, the areas of stable parameter space according to different α values and τ = 1.6 are calculated as 444.8860 for α = 0.4 , 342.9728 for α = 0.7 , 259.3578 for α = 1 , 216.2541 for α = 1.3 and 159.6826 for α = 1.6 . In addition, the areas of stable parameter space according to different τ values and α = 1.4 are calculated as 784.5222 for τ = 1 , 106.3219 for τ = 2 , 29.6959 for τ = 3 and 11.5946 for τ = 4 . Despite the extreme variability arising from nature of resources that make up the MG, the designed non integer order controller with the values selected within the stable parameter space stably carries out LFC control of the MG.     

Mechanical properties and repair bond strength of polymer-based CAD/CAM restorative materials

Information on the mechanical properties and repairability of computer-aided design/computer-aided manufacturing (CAD/CAM) blocks is scarce. Five CAD/CAM blocks with similar indications were evaluated, including four resin nanoceramics, one polymer-infiltrated ceramic network (PICN), and one feldspathic ceramic. CAD/CAM blocks were sectioned into 4 mm × 1.2 mm × 13 mm bars for flexural strength (FS), and 4 mm thick blocks were prepared for elastic modulus (EM), nanohardness (NH), and microshear bond strength (µSBS) testing. FS of the CAD/CAM blocks was determined using a three-point bending test, whereas EM and NH values were measured using a nanoindenter. The reparability of the tested block materials was determined by the µSBS test. One-way ANOVA was conducted for FS, EM, NH, and µSBS followed by Tukey's pairwise comparison (α < 0.05). FS ranged from 115 to 207 MPa, EM from 8.21 to 44 GPa, NH from 0.76 to 7.24, and µSBS from 24.9 to 30.6 MPa. The findings of the present study revealed that resin nanoceramic blocks exhibited higher FS values than PICN and feldspathic ceramic, and they have acceptable mechanical properties for the fabrication of single-unit restorations according to the ISO 6872:2008. Furthermore, all CAD/CAM blocks tested can be successfully repaired regarding their recommended repair protocol.     

The effect of rock weathering and transition zones on the performance of an EPB-TBM in complex geology near Istanbul,Turkey

Bulletin of Engineering Geology and the Environment - The new Istanbul Airport is being constructed over an old coal field area, 35 km away from the city center. The construction of a rail link...     

Porous 3D printed concrete beams show an environmental promise: a cradle-to-grave comparative life cycle assessment

3D Concrete Printing (3DCP) is a rapidly expanding area in the field of architecture, engineering, and construction, but very limited research has quantitatively investigated its environmental impact. The existing Life Cycle Assessment (LCA) studies on 3DCP lack clearly defined functional units of comparison, especially considering load-bearing structures. This paper investigates the potential environmental benefits of 3DCP over conventional concrete construction for structural beams based on a cradle-to-grave comparative LCA. Unlike existing studies, this paper employs a recarbonation model to account for the carbon offsetting from the use-stage of 3DP concrete, which shows significant results. The assessment includes three-beam designs, each analyzed for both prefabrication and on-site construction scenarios. While currently, 3DCP has a generally higher environmental impact due to the larger quantity of cement employed in the process, the reduction of material through infill optimization for printed beams is a promising design principle to positively offset the environmental impacts in the construction sector. The paper draws recommendations for future research on material- and recarbonation-efficient 3DCP design for load-bearing structures, as well as on material development, e.g. integration of larger aggregates and low-clinker cement.

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