Several herbivorous caterpillars contain effectors in their oral secretions that alter the emission of green leaf volatiles (GLVs) produced by the plants upon which the caterpillars are feeding. These effectors include an isomerase, a fatty acid dehydratase (FHD), and a heat-stable hexenal trapping (HALT) molecule. GLVs serve as signaling compounds in plant-insect interactions and inter-and intra-plant communication. However, it is not known whether these GLV-altering effectors are common among herbivorous caterpillars, or the evolutionary context of these effectors in relation to GLV emission by host plants in response to feeding damage. Here, we examined the distribution and activity of the isomerase, FHD, and HALT effectors across 10 species spanning 7 lepidopteran families. Six of the 10 species possessed all three effectors in their oral secretions. Activity from the HALT and FHD effectors was observed in all examined caterpillar species, while activity from the isomerase effector varied in some species and was absent in others. There was no discernable pattern in effector activity based on evolutionary divergence, since individual species within a family did not possess similar mechanisms to alter GLV emission. These data, demonstrating the GLV-altering effectors acting at different steps in the GLV biosynthetic pathway and present in the examined caterpillar species at different combinations with different activities, highlight the importance of these effectors in changing the emission of these compounds during caterpillar herbivory. Understanding the prevalence and roles of GLV-altering effectors and GLV emission itself will open new research areas in the dynamics of plant-insect interactions.
Targeting the tumor cell mitochondrion could produce novel anticancer agents. We designed an aryl−urea fatty acid ( 1 g ; 16({[4-chloro-3-(trifluoromethyl)phenyl]carbamoyl}amino)hexadecanoic acid) that disrupted the mitochondrion and decreased MDA-MB-231 breast cancer cell viability. To optimize the aryl−ureas the present study evaluated mitochondrial targeting by 1 g analogues containing alkyl chains between 10–17 carbons. Using the dye JC-1, the C12−C17 analogues efficiently disrupted the mitochondrial membrane potential (IC50s 3.5±1.2 to 7.6±1.1 μM) and impaired ATP production; shorter analogues were less active. 7-Aminoactinomycin D/annexin V staining and flow cytometry showed that these agents activated the killing mechanisms of necrosis and apoptosis to varying extents (7-aminoactinomycin D/annexin V staining ratios 4.3–6.0). Indeed, 1 g and its C17 analogue preferentially activated necrosis and apoptosis, respectively (ratios 2.1 and 16). Taken together, alkyl chain length is a determinant of mitochondrial targeting by aryl−ureas and can be varied to develop analogues that activate apoptosis or necrosis in a regulated fashion. 相似文献
Incineration has suffered from a sustained campaign of misinformation. Consequently it has been regarded as the least sustainable option for the treatment of wastes, including sewage sludge. By looking carefully at the issues surrounding the incineration of sewage sludge, as an example, it can be seen that reality does not support the commonly held view. Modern plants are an effective means of recovering the energy value of sludge and at the same time offer a continuous operation, which is independent of weather or land constraints. Of course incineration needs to be regulated and EU Directives and National Regulations set the emission standards. The technology is such that these standards can be met, and more than this, the systems are capable of upgrading as more stringent standards are imposed. Concerns over NOx, dioxins, metals and pathogens are highlighted. In addition the permitting process is discussed. None of these should present a barrier to the implementation of incineration projects. Indeed the paper goes further and demonstrates that there is likely to be an increasing role for incineration as landfill, recycling to agricultural land and other options become more restrictive. 相似文献
River water has been sampled under baseflow conditions in both summer and winter at c. 250-m intervals along the length of each of seven rivers in northeast Scotland, and the nitrate contents have been determined. The resultant data have been examined in the context of the nitrogen saturation hypothesis. Capacity of the catchment soils to retain nitrate was generally minimal in winter. Although biological uptake of nitrate was evident in summer, in the upper part of each catchment substantial nitrate leaching still occurred. The phenomenon was most marked in water draining from hill peats, except where these were conspicuously waterlogged. The results are discussed in the context of possible nitrogen saturation effects on upper catchment slopes. 相似文献
The magnitude and implications for the construction industry of reductions in carbon dioxide emissions that will ultimately be required globally and in industrialized countries are critically examined. As a result of the problem of climate change, these reductions are found to be in the region of 80–90% by 2050- much larger than those presently under discussion in national and global political fora. The technological feasibility of buildings with low energy requirements and low carbon emissions suggests that existing technology is capable of delivering reductions in this range. Unfortunately, the progress that is being made at the cutting edge is, with very few exceptions, not carried over into building stocks as a whole. Regulatory, fiscal and market-based policy and strategy mechanisms for improving the performance both of new build and the existing stock are critically examined. The achievement of meaningful reductions will require a coordinated and strategic approach, which makes use of the complementarity between these mechanisms. Regulation alone, in the absence of clear and consistent price signals provided through mechanisms such as carbon taxation, is unlikely to deliver the reductions in carbon emissions that will be needed to stabilize the global atmosphere and climate. L'ampleur et les implications pour l'industrie de construction des réductions des émissions de dioxyde de carbone qui seront généralement exigées en fin de compte font l'objet d'un examen critque dans les pays industrialisés. Suite au problème des changements de climat, on a trouvé qu'en 2050 ces réductions d'émissions devraient se situer dans une zone de 80 à 90 % - c'est à dire, beaucoup plus grands que celle qu'on trouve dans les discussions et symposiums politiques nationaux et mondiaux. La faisabilité technologique de constructions nécessitant peu d'énergie et à faible émission de carbone suggére que la technologie actuelle est capable d'apporter des solutions à ces réductions. Malheureusement, à de rares exceptions prés, les progrés qui sont faits dans ces domaines de pointe ne sont pas appliqués dans leur totalité au secteur du bâtiment. La politique coordinatrice et fiscale, basée sur le marché ainsi que les mécanismes stratégiques visant à améliorer les performances concernant aussi bien les nouvelles constructions que celles existantes, sont examinés d'une façon critique. L'obtention de réductions significatives exigera une approche coordonnée et stratégique mettant en oeuvre ces deux mécanismes complémentaires. La seule réglementation, en l'absence d'arguments de prix clairs et cohérents comme par exemple la taxation sur l'oxyde de carbone, ne va vraisemblablement pas entrâner les réductions d'émissions de gaz carbonique qui seraient nécessaires afin de stabilizer l'atmosphère et le climat au niveau mondial. 相似文献
Selective isolation of Kunitz trypsin inhibitor (KTI) and lectin from soybean whey solutions by different types of chitosan beads was investigated. The chitosan beads were co-crosslinked with tripolyphosphate/genipin in solutions at pH 5, 7 or 9 (CB5, CB7, CB9). The maximum adsorption ratios of chitosan beads to KTI and lectin were observed at pH 4.4 and 5.4, respectively; highly selective separation was also demonstrated at these pHs. The adsorption ratios increased with temperature, rising between 5 and 25 °C. CB9 produced the best adsorption ratio, followed by CB7 then CB5. The critical interaction governing absorption of chitosan beads to KTI and lectin could be hydrogen bonding. At pH 9, KTI and lectin desorbed efficiently from CB7 with desorption ratios of 80.9% and 81.4%, respectively. The desorption was most likely caused predominantly by electrostatic repulsion. KTI and lectin can effectively be selectively isolated from soybean whey using this novel separation technique. 相似文献
Little detailed evidence has previously been available regarding the uptake rate or prevalence of energy efficiency interventions among specific household groups. This study uses the Home Energy Efficiency Database (HEED) to investigate both the combination of measures that have been installed, and in which dwellings, according to key neighbourhood socio-demographic variables, including income and tenure. Analysis of 2000–07 data indicates that approximately 40% (9.3 million) dwellings in England had approximately 23.7 million efficiency measures installed, with an average of 2.5 measures per dwelling. Building fabric-related measures were the most frequent (e.g. cavity wall insulation, loft insulation and glazing) with an average of 2.1 million installed each year. Dwellings with the highest number of fabric interventions (the top 20%) were more likely to be found in areas with low income, with more owner-occupied dwellings, experiencing lower winter temperatures, having a lower proportion of flats, and having a slightly higher proportion of older adults and children. Energy efficiency installations have tended to occur among specific types of households or parts of the building stock. These findings have implications for the design of future government programmes for targeting energy efficiency measures to specific household groups or dwelling types. 相似文献