Real-time organic aerosol chemical speciation in the indoor environment using extractive electrospray ionization mass spectrometry

Understanding the sources and composition of organic aerosol (OA) in indoor environments requires rapid measurements, since many emissions and processes have short timescales. However, real-time molecular-level OA measurements have not been reported indoors. Here, we present quantitative measurements, at a time resolution of five seconds, of molecular ions corresponding to diverse aerosol-phase species, by applying extractive electrospray ionization mass spectrometry (EESI-MS) to indoor air analysis for the first time, as part of the highly instrumented HOMEChem field study. We demonstrate how the complex spectra of EESI-MS are screened in order to extract chemical information and investigate the possibility of interference from gas-phase semivolatile species. During experiments that simulated the Thanksgiving US holiday meal preparation, EESI-MS quantified multiple species, including fatty acids, carbohydrates, siloxanes, and phthalates. Intercomparisons with Aerosol Mass Spectrometer (AMS) and Scanning Mobility Particle Sizer suggest that EESI-MS quantified a large fraction of OA. Comparisons with FIGAERO-CIMS shows similar signal levels and good correlation, with a range of 100 for the relative sensitivities. Comparisons with SV-TAG for phthalates and with SV-TAG and AMS for total siloxanes also show strong correlation. EESI-MS observations can be used with gas-phase measurements to identify co-emitted gas- and aerosol-phase species, and this is demonstrated using complementary gas-phase PTR-MS observations.     

Deliberately Losing Control of C−H Activation Processes in the Design of Small-Molecule-Fragment Arrays Targeting Peroxisomal Metabolism

Combined photochemical arylation, “nuisance effect” (S_NAr) reaction sequences have been employed in the design of small arrays for immediate deployment in medium-throughput X-ray protein–ligand structure determination. Reactions were deliberately allowed to run “out of control” in terms of selectivity; for example the ortho-arylation of 2-phenylpyridine gave five products resulting from mono- and bisarylations combined with S_NAr processes. As a result, a number of crystallographic hits against NUDT7, a key peroxisomal CoA ester hydrolase, have been identified.     

Heat Killed Lactobacillus reuteri GMNL-263 Reduces Fibrosis Effects on the Liver and Heart in High Fat Diet-Hamsters via TGF-β Suppression

Obesity is one of the major risk factors for nonalcoholic fatty liver disease (NAFLD), and NAFLD is highly associated with an increased risk of cardiovascular disease (CVD). Scholars have suggested that certain probiotics may significantly impact cardiovascular health, particularly certain Lactobacillus species, such as Lactobacillus reuteri GMNL-263 (Lr263) probiotics, which have been shown to reduce obesity and arteriosclerosis in vivo. In the present study, we examined the potential of heat-killed bacteria to attenuate high fat diet (HFD)-induced hepatic and cardiac damages and the possible underlying mechanism of the positive effects of heat-killed Lr263 oral supplements. Heat-killed Lr263 treatments (625 and 3125 mg/kg-hamster/day) were provided as a daily supplement by oral gavage to HFD-fed hamsters for eight weeks. The results show that heat-killed Lr263 treatments reduce fatty liver syndrome. Moreover, heat-killed Lactobacillus reuteri GMNL-263 supplementation in HFD hamsters also reduced fibrosis in the liver and heart by reducing transforming growth factor β (TGF-β) expression levels. In conclusion, heat-killed Lr263 can reduce lipid metabolic stress in HFD hamsters and decrease the risk of fatty liver and cardiovascular disease.     

Porphyrin polymers and organic frameworks

The versatility of porphyrins as optoelectronic or catalytic units makes them attractive elements for inclusion in functional materials. Polymers that include porphyrins have been created with a wide variety of structures and used in a wide range of applications. This review covers recent developments in the synthesis, characterization and applications of polymeric materials in which porphyrins are key components of the repeat units, including the rapidly growing area of metal–organic frameworks and related covalent organic frameworks. © 2015 Society of Chemical Industry     

Cure characterization of Cycom 977‐2A carbon/epoxy composites for quickstep processing

In this effort, Quickstep, a relatively a new technique, have been employed for manufacturing of composite materials. The cure schedule provided by a prepreg manufacturer is usually designed for autoclave or other traditional processing techniques and thermosetting resin systems are formulated for ramp rate curing 2–3 K min^?1. While in case of Quickstep processing, ramp rates of 15 K min^?1 can be achieved, thus changing the chemorheology of resin. The cure process of 977‐2A carbon/epoxy composites was evaluated for Quickstep processing using differential scanning calorimetry (DSC), dynamic mechanical and thermal analysis, and Fourier transformed infrared and results were compared with cure cycle employed for autoclave curing. Optimum hold time for Quickstep processing at upper curing temperature (180°C) was determined using DSC. The hold time of 120 min at 180°C was found to be suitable for Quickstep cure cycle, producing a panel of similar degree of cure to that achieved through autoclave processing schedule. Final degree of cure was dependent on time spent at upper cure temperature and slightly on initial steps of the cure cycle which was used to control the resin flow, fiber wetting, and void removal. Quickstep processed samples exhibited higher T_g and crosslink density and similar molecular network structure to the autoclave cured samples. POLYM. ENG. SCI., 54:887–898, 2014. © 2013 Society of Plastics Engineers     

Thermal radiative protection of fire fighters' protective clothing

A method for measuring the thermal radiative protection of actual fire fighters' garments to an incident radiative heat flux of 8.4 kW/m² is described. Typical results obtained with several conventional and prototype garments are presented. These results indicate the time to pain, and second degree burn as well as the pain alarm time. The thermal inertia of the garments is also measured based upon burn exposure time. Differences in physical properties such as garment thickness, total weight and number of layers are examined in order to establish the existence of any correlations.Issued as NRCC 26171. Reference: M. Day and P. Z. Sturgeon, Thermal Radiative Protection of Fire Fighters' Protective Clothing,Fire Technology, Vol. 23, No. 1, February 1987, p. 49.     

Combined use of an electrostatic precipitator and a high‐efficiency particulate air filter in building ventilation systems: Effects on cardiorespiratory health indicators in healthy adults

High‐efficiency particulate air (HEPA) filtration in combination with an electrostatic precipitator (ESP) can be a cost‐effective approach to reducing indoor particulate exposure, but ESPs produce ozone. The health effect of combined ESP‐HEPA filtration has not been examined. We conducted an intervention study in 89 volunteers. At baseline, the air‐handling units of offices and residences for all subjects were comprised of coarse, ESP, and HEPA filtration. During the 5‐week long intervention, the subjects were split into 2 groups, 1 with just the ESP removed and the other with both the ESP and HEPA removed. Each subject was measured for cardiopulmonary risk indicators once at baseline, twice during the intervention, and once 2 weeks after baseline conditions were restored. Measured indoor and outdoor PM_2.5 and ozone concentrations, coupled with time‐activity data, were used to calculate exposures. Removal of HEPA filters increased 24‐hour mean PM_2.5 exposure by 38 (95% CI: 31, 45) μg/m³. Removal of ESPs decreased 24‐hour mean ozone exposure by 2.2 (2.0, 2.5) ppb. No biomarkers were significantly associated with HEPA filter removal. In contrast, ESP removal was associated with a ?16.1% (?21.5%, ?10.4%) change in plasma‐soluble P‐selectin and a ?3.0% (?5.1%, ?0.8%) change in systolic blood pressure, suggesting reduced cardiovascular risks.     

Quantification and source characterization of volatile organic compounds from exercising and application of chlorine-based cleaning products in a university athletic center

Humans spend approximately 90% of their time indoors, impacting their own air quality through occupancy and activities. Human VOC emissions indoors from exercise are still relatively uncertain, and questions remain about emissions from chlorine-based cleaners. To investigate these and other issues, the ATHLETic center study of Indoor Chemistry (ATHLETIC) campaign was conducted in the weight room of the Dal Ward Athletic Center at the University of Colorado Boulder. Using a Vocus Proton-Transfer-Reaction Time-of-Flight Mass Spectrometer (Vocus PTR-TOF), an Aerodyne Gas Chromatograph (GC), an Iodide-Chemical Ionization Time-of-Flight Mass Spectrometer (I-CIMS), and Picarro cavity ringdown spectrometers, we alternated measurements between the weight room and supply air, allowing for determination of VOC, NH₃, H₂O, and CO₂ emission rates per person (emission factors). Human-derived emission factors were higher than previous studies of measuring indoor air quality in rooms with individuals at rest and correlated with increased CO₂ emission factors. Emission factors from personal care products (PCPs) were consistent with previous studies and typically decreased throughout the day. In addition, N-chloraldimines were observed in the gas phase after the exercise equipment was cleaned with a dichlor solution. The chloraldimines likely originated from reactions of free amino acids with HOCl on gym surfaces.