Two Triterpene Synthases from Imperata cylindrica Catalyzing the Formation of a Pair of Diastereoisomers through Boat or Chair Cyclization

Imperata cylindrica is known to produce a pair of triterpenes, isoarborinol and fernenol, that exhibit identical planar structures but possess opposite stereochemistry at six of the nine chiral centers. These differences arise from a boat or a chair cyclization of the B-ring of the substrate. Herein, we report the characterization of three OSC genes from I. cylindrica. IcOSC1 and IcOSC5 were identified as isoarborinol and fernenol synthases, respectively, while IcOSC3 was characterized as a multifunctional enzyme that produces glutinol and friedelin as its major products. Mutational studies of isoarborinol and fernenol synthases revealed that the residues surrounding the DCTAE motif partially affected the conformation of the B-ring during cyclization. Additionally, the IcOSC1-W255H mutant produced the rare triterpene boehmerol. The introduced histidine residue presumably abstracted a proton from the intermediary carbocation at C18 during the 1,2-rearrangement. Expression analysis indicated that all OSC genes were highly expressed in stems.     

Effect of Shear Rate Distribution on Particle Aggregation in a Stirred Vessel

The modeling of particle aggregation under a simple shear flow and the extension of the model to a stirred vessel is described. The model quantitatively demonstrates the change of the number of aggregates with time for each shear rate. This number increased with higher shear rate and, conversely, the aggregate size became small when raising the shear rate. This was because aggregates were broken by the stronger shear force. The number of aggregates for different impellers was determined. The shear rate was back‐calculated from the experimentally obtained aggregate size and the model equation. This shear rate was different from that estimated from the Metzner‐Otto method, consequently, some revisions of the Metzner‐Otto equation might be necessary for its application to particle aggregation.     

Locomotion Control of MEMS Microrobot Using Pulse‐Type Hardware Neural Networks

This paper presents the locomotion control of a microelectromechanical system (MEMS) microrobot. The MEMS microrobot demonstrates locomotion control by pulse‐type hardware neural networks (P‐HNN). P‐HNN generate oscillatory patterns of electrical activity like those of living organisms. The basic component of P‐HNN is a pulse‐type hardware neuron model (P‐HNM). The P‐HNM has the same basic features as biological neurons, such as the threshold, the refractory period, and spatiotemporal summation characteristics, and allows the generation of continuous action potentials. P‐HNN has been constructed with MOSFETs and can be integrated by CMOS technology. Like living organisms, P‐HNN has realized robot control without using software programs or A/D converters. The size of the microrobot fabricated by MEMS technology was 4 × 4 × 3.5 mm. The frame of the robot was made of a silicon wafer, equipped with rotary actuators, link mechanisms, and six legs. The MEMS microrobot emulated the locomotion method and the neural networks of an insect by rotary actuators, link mechanisms, and the P‐HNN. We show that the P‐HNN can control the forward and backward locomotion of the fabricated MEMS microrobot, and that it is possible to switch its direction by inputting an external trigger pulse. The locomotion speed was 19.5 mm/min and the step size was 1.3 mm. © 2013 Wiley Periodicals, Inc. Electr Eng Jpn, 186(3): 43–50, 2014; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.22473     

Effects of Particle Strength of Feedstock Powders on Properties of Warm-Sprayed WC-Co Coatings

Warm spray (WS) process, which can control the temperature of a combustion gas jet used to propel powder, has been successfully applied to deposit WC-Co coatings. Detrimental reactions resulting from dissolution of WC into Co binder and decarburization were suppressed effectively by keeping the WC-Co particles’ temperature below the m.p. of the binder phase. In this study, three nano-structured WC-12Co powders with different particle strengths were prepared by changing the sintering conditions of spray-dried powder and were deposited by WS. The deposition efficiency and porosity of the coatings decreased with increasing the particle strength. The coating deposited from the powder with very low particle strength showed significant phase changes, while those deposited from the higher particle strengths showed almost no change. Particle Image Velocimetry revealed significant disintegration of the weakest powder, which explains the changes observed. The hardness and wear properties of the former coating, therefore, were inferior to the other two.     

Modulation of nanotube formation in apatite single crystal via organic molecule incorporation

Hydroxyapatite materials are potentially useful for biomedical application, especially as vehicles for functional molecules. Structural control of bulk apatite materials, such as in the fabrication of hollow microspheres or porous structures, has been studied for this purpose. However, control of the internal structure of the source apatite crystal itself is still a challenge. Here, we show that small organic molecules incorporated in apatite crystals act as porogens which control the porous structure of apatite single crystal. The presence of amino acid under apatite synthesis conditions leads to firm bindings and encapsulation of the amino acid in apatite single crystals. Amino acid elimination by heating or electron beam irradiation enhances the pore formation in apatite single crystal. Moreover, incorporation of an acidic amino acid in apatite induces peapod like nanotubes in apatite single crystals. This study suggests the potential of using small organics for nano-structural control of apatite single crystals which would be valuable for enhancing drug loadings or modulating material digestion in vivo.     

A classification control strategy for energy storage system in microgrid

Storage devices are indispensable elements in a microgrid to compensate for the power imbalance between loads and the distributed generator (DG) output. Different storage strategies give diverse performances in adjustment speed and capacity. Based on the performance of different storage devices and the features of power imbalance curve in different periods, a classification control strategy is proposed in this paper. First, storage devices are given priorities according to the adjusting speed, and the power imbalance curve is divided into two periods according to the changing speed. During an emergency, all the storages are employed to compensate the rapidly increasing power imbalance; during the steady period, storage device with higher priority is employed to track the instantaneous component of power imbalance, while storage device with lower priority is used to replace the kWh output of higher priority storage. The proposed strategy is tested in a microgrid within the subdistribution network of IEEE RBTS Bus6 system using Monte Carlo methods. Simulation results indicate that the classification control strategy could endow the system with fast adjusting ability through coordination between storages. Reliability indices of the testing system are utilized to demonstrate that the classification control strategy could improve the stability of the microgrid and reliability of the distribution network. © 2015 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.     

Magnetically Ordered State of Cold Fermions on a Decorated Square Lattice

We study two-component ultracold fermions with repulsive interactions, which are loaded into a decorated square lattice. By combining the real-space dynamical mean-field theory with the numerical renormalization group method, we discuss magnetic properties in the system. It is clarified how the ferromagnetically ordered ground state, which is stabilized by a flat band mechanism, is adiabatically connected to the ferrimagnetically ordered state expected in the strong coupling limit.     

Developed Modeling and Numerical Simulation for Mismatching Photovoltaic Performance Evaluation

A simplified simulation method was described for charactering mismatching photovoltaic performance integrated with bypass and string diodes, which are configured additionally compared with the tradition design for avoiding deterioration to modules in series or in parallel connection. The Newton-Raphson method was adopted for numerical simulations, with some computation problem discussed for fast convergence. It was proven to be suitable and effective when model validation coinciding with manufacturer specification. Furthermore, maximal power point (MPP) peak positions were reviewed roundly via simulations of the total characteristic curves. Multi-power peaks with multi-diodes installed were also investigated. The proposed method can be benefit for better comprehension on the physical circuit and provides greater program expansibility, and such simulation researches should be applicable for manufacturer to evaluate the configuration of photovoltaic module. © 2009 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.