全文获取类型
收费全文 | 860篇 |
免费 | 2篇 |
学科分类
工业技术 | 862篇 |
出版年
2019年 | 5篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2013年 | 1篇 |
2010年 | 2篇 |
2009年 | 1篇 |
2008年 | 3篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 1篇 |
2002年 | 1篇 |
2001年 | 2篇 |
1999年 | 23篇 |
1998年 | 229篇 |
1997年 | 125篇 |
1996年 | 93篇 |
1995年 | 55篇 |
1994年 | 51篇 |
1993年 | 53篇 |
1992年 | 8篇 |
1991年 | 12篇 |
1990年 | 15篇 |
1989年 | 16篇 |
1988年 | 16篇 |
1987年 | 14篇 |
1986年 | 8篇 |
1985年 | 14篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 4篇 |
1980年 | 12篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 21篇 |
1976年 | 55篇 |
1975年 | 4篇 |
1964年 | 1篇 |
排序方式: 共有862条查询结果,搜索用时 15 毫秒
1.
2.
AK Metzger G Mohapatra YA Minn AW Bollen K Lamborn FM Waldman CB Wilson BG Feuerstein 《Canadian Metallurgical Quarterly》1999,90(2):306-314
OBJECT: This study was conducted to determine whether comparative genomic hybridization (CGH) is a more sensitive method for detecting genetic aberrations than other tests currently in use. METHODS: The authors used CGH to examine 40 primary and 13 recurrent adenomas obtained from 52 patients for loss and gain of genetic material. Copy number aberrations (CNAs) were detected in 25 (48%) of the 52 patients studied. The chromosomes affected were, in order of decreasing frequency, 11, 7, X, 1, 8, 13, 5, 14, 2, 6, 9, 10, 12, 3, 18, 21, 4, 16, 15, 19, 22, and Y. Endocrinologically active adenomas were more likely to contain (p = 0.009) and had a greater number (p = 0.003) of CNAs. Of 26 adenomas with CNAs, 18 showed multiple aberrations involving entire chromosomes or chromosome arms. The most frequent CNA involving a chromosome subregion, which was present in four (8%) of 53 adenomas, was the loss of all chromosome 11 material except for a preserved common segment containing 11q13. Immunoperoxidase staining did not detect cyclin D1 expression in those four cases, making cyclin D1 an unlikely target of this rearrangement. CONCLUSIONS: These findings indicate that genetic abnormalities are present in pituitary adenomas at a higher rate than previously reported, are associated with endocrinological activity, and often involve several chromosomes. Rearrangement at 11q13 may inactivate a tumor suppressor gene or activate an oncogene that is important in the initiation or progression of sporadic pituitary adenomas. 相似文献
3.
NK lysin is a 9-kDa polypeptide that was originally isolated from porcine intestinal tissue based on its antibacterial activity. It is produced by cytolytic lymphocytes and is cytolytic against a number of different types of tumor cells. Here we report the binding of NK lysin to lipopolysaccharide (LPS) and its anti-LPS activity. NK lysin binds to matrix-coated LPS from Escherichia coli, Pseudomonas aeruginosa, and different strains of Salmonella enterica. Lipid A and polymyxin B inhibited the binding, demonstrating a preferential interaction of NK lysin with the lipid part of LPS. Chromium-labeled lymphoma cells were lysed by NK lysin, and LPS dose-dependently inhibited the cytolysis at equimolar amounts. In the same manner, NK lysin inhibited certain LPS-stimulated effects on mouse bone marrow cells as well as LPS binding to mouse granulocytes. These results suggest that NK lysin may be a another natural LPS-binding protein from lymphocytes that may participate in the endogenous defense response associated with elevated concentrations of LPS. 相似文献
4.
5.
Bauschke H.H. Noll D. Celler A. Borwein J.M. 《IEEE transactions on medical imaging》1999,18(3):252-261
In this paper we present two variants of the EM algorithm for dynamic SPECT imaging. A version based on compartmental modeling which fits a sum of exponentials and a more general approach allowing for arbitrary decaying activities. The underlying probabilistic models are discussed and the incomplete and complete data spaces are shown to be physically meaningful. We indicate that the second method, leading to a convex program in the M step, is easier to treat numerically and we present a possible numerical approach. Some preliminary numerical tests indicating the feasibility of the method are included. 相似文献
6.
LG Kupchinski? IuA Barshte?n BG Pogodaev AI Treshinski? 《Canadian Metallurgical Quarterly》1976,(3):80-84
A study was made of a possibility of using Soviet made membranes "Vladipor" with a pore diameter of 16--17 nm for the purpose of concentration and purification of antigenic extracts from stab cultures of S. typhi obtained by the action of typsin, hydrogen peroxide and detergents. Preparations, complete by antigenic structure, were obtained by ultrafiltration, with a good yield. The maximal rate of ultrafiltration was noted in case of "peroxide" antigenic preparations. The use of diafiltration provided a significant reduction of the ash content in the end products. 相似文献
7.
OH Willemsen MM Snel L Kuipers CG Figdor J Greve BG De Grooth 《Canadian Metallurgical Quarterly》1999,76(2):716-724
Atomic force microscopy is one of the few techniques that allow analysis of biological recognition processes at the single-molecule level. A major limitation of this approach is the nonspecific interaction between the force sensor and substrate. We have modeled the nonspecific interaction by looking at the interaction potential between a conical Si3N4 tip with a spherical end face and a mica surface in solution, using DLVO (Derjaguin, Landau, Verwey, Overbeek) theory and numerical calculations. Insertion of the tip-sample potential in a simulation of an approach-retract cycle of the cantilever gives the well-known force-distance curve. Simulating a force-distance curve at low salt concentration predicts a discrete hopping of the tip, caused by thermal fluctuations. This hopping behavior was observed experimentally and gave rise to a novel approach to making measurements in adhesion mode that essentially works in the repulsive regime. The distance between tip and sample will still be small enough to allow spacer-involved specific interactions, and the percentage of nonspecific interactions of the bare tip with the mica is minimized. We have validated this physical model by imaging intercellular adhesion molecule 1 (ICAM-1) antigen with a tip functionalized with anti-ICAM-1 antibody. The measurement demonstrated that a significant decrease in the number of nonspecific interactions was realized, and the topographical image quality and the specific bonding capability of the tip were not affected. 相似文献
8.
9.
10.