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1.
Experimental results are presented to show that the adhesion force is the single most important limiting factor in high-resolution atomic force microscopy of DNA in air, prepared by the cytochrome-C-assisted spreading method. It is also shown that humidity plays a minor role in the control of probe force. Using a pure carbon film as the substrate to clean the AFM tip prior to imaging, it is demonstrated that 4-6 nm resolution on DNA can be routinely obtained by the atomic force microscope with commercial Si3N4 pyramid cantilevers. We also show that in organic solvents a resolution of up to 3 nm can be obtained under optimal conditions. 相似文献
2.
We present experimental results on the phase equilibrium of a saturated3He−4He mixture, which has been cooled to a temperature of 10–15 mK and polarized in a4He circulating dilution refrigerator to a stationary polarization of 15%, 7 times higher than the equilibrium polarization
in the external field of 7 T. The pressure dependence of the polarization enhancement in the refrigerator shows that the molar
susceptibilities of the concentrated and dilute phase of a saturared3He-4He mixture are equal atp=2.60±0.04 bar. This result affects the Fermi liquid parameters of the dilute phase. The osmotic pressure in the dilute phase
has been measured as a function of the polarization of the coexisting concentrated phase up to 15%. We find that the osmotic
pressure at low polarization (<7%) agrees well with thermodynamics using the new Fermi liquid parameters of the dilute phase. 相似文献
3.
Insulin-like growth factor I activates the invasion suppressor function of E-cadherin in MCF-7 human mammary carcinoma cells in vitro 总被引:1,自引:0,他引:1
ME Bracke BM Vyncke EA Bruyneel SJ Vermeulen GK De Bruyne NA Van Larebeke K Vleminckx FM Van Roy MM Mareel 《Canadian Metallurgical Quarterly》1993,68(2):282-289
The calcium-dependent cell-cell adhesion molecule E-cadherin has been shown to counteract invasion of epithelial neoplastic cells. Using three monoclonal antibodies, we have demonstrated the presence of E-cadherin at the surface of human MCF-7/6 mammary carcinoma cells by indirect immunofluorescence coupled to flow cytometry and by immunocytochemistry. Nevertheless, MCF-7/6 cells failed to aggregate in a medium containing 1.25 mM CaCl2, and they were invasive after confrontation with embryonic chick heart fragments in organ culture. Treatment of MCF-7/6 cells with 0.5 microgram ml-1 insulin-like growth factor I (IGF-I) led to homotypic aggregation within 5 to 10 min and inhibited invasion in vitro during at least 8 days. The effect of IGF-I on cellular aggregation was insensitive to cycloheximide. However, monoclonal antibodies that interfered with the function of either the IGF-I receptor (alpha IR3) or E-cadherin (HECD-1, MB2) blocked the effect of IGF-I on aggregation. The effects of IGF-I on aggregation and on invasion could be mimicked by 1 microgram ml-1 insulin, but not by 0.5 microgram ml-1 IGF-II. The insulin effects were presumably not mediated by the IGF-I receptor, since they could not be blocked by an antibody against this receptor (alpha IR3). Our results indicate that IGF-I activates the invasion suppressor role of E-cadherin in MCF-7/6 cells. 相似文献
4.
5.
The interactive editing and contouring of empirical fields 总被引:2,自引:0,他引:2
Dickinson R.R. Bartels R.H. Vermeulen A.H. 《Computer Graphics and Applications, IEEE》1989,9(3):34-43
A system for the interactive editing and contouring of surfaces derived from empirical fields is described. The approach taken begins with the representation of a field as a general-order, nonuniform, tensor-product, B-spline surface. It provides an interactive display for editing the surface by control-vertex manipulation and a contouring algorithm that is specifically designed for the fast and robust contouring of B-spline surfaces. Interactive editing of the resulting model is feasible because of the local nature of editing changes when B-splines are used. The use of nonuniform B-splines gives the flexibility required to model highly irregular data efficiently 相似文献
6.
This study was designed to test the hypotheses that digestibility and post-absorption metabolism of fish oil are influenced
by impaired lipolysis and by the stereospecific composition of its triacylglycerols. Male Wistar rats were fed nonpurified
diets containing one of the following fat sources: 9% native fish oil (NFO), 9% autorandomized fish oil (RFO), 8.1% fish oil-derived
free fatty acids (FO-FFA) plus 0.9% glycerol, or 9% soybean oil (SO) as a reference fat. In a 24-day balance study, apparent
digestibility of total dietary fat averaged 93.1% in the SO, NFO and RFO groups, and 90.9% in the FO-FFA group. Randomization
of fish oil had no effect on apparent digestibility of individual fatty acids. In rats fed FO-FFA, apparent absorption of
saturated and monounsaturated fatty acids was lower when compared to the NFO and RFO groups. Feeding the FO-FFA diet tended
to increase plasma triglyceride content. The hypocholesterolemic effect of polyunsaturated n−3 fatty acids was not influenced
by the dietary source. Similar effects on fatty acid profiles of plasma and liver phospholipids were caused by the NFO, RFO
and the FO-FFA diets. We conclude that once polyunsaturated n−3 fatty acids are absorbed, their effect on lipid metabolism
is not determined by the dietary source. 相似文献
7.
W. G. Vermeulen P. J. van der Wolk A. P. de Weijer S. van der Zwaag 《Journal of Materials Engineering and Performance》1996,5(1):57-63
Jominy hardness profiles of steels were predicted from chemical composition and austenitizing temperature using an artificial
neural network. The neural network was trained using some 4000 examples, covering a wide range of steel compositions. The
performance of the neural network is examined as a function of the network architecture, the number of alloying elements,
and the number of data sets used for training. A well-trained network predicts the Jominy hardness profile with an average
error of about 2 HRC. Special attention was devoted to the effect of boron on hardenability. A network trained using data
only from boron steels produced results similar to those of a network trained using all data available. The accuracy of the
predictions of the model is compared with that of an analytical model for hardenability and with that of a partial least-
squares model using the same set of data. 相似文献
8.
Dries Schellekens Brecht Wyseur Bart Preneel 《Electronic Notes in Theoretical Computer Science》2008,197(1):59
A lot of progress has been made to secure network communication, e.g., through the use of cryptographic algorithms. However, this offers only a partial solution as long as the communicating end points still suffer from security problems. A number of applications require remote verification of software executing on an untrusted platform. Trusted computing solutions propose to solve this problem through software and hardware changes, typically a secure operating system and the addition of a secure coprocessor respectively. On the other hand, timed execution of code checksum calculations aims for a solution on legacy platforms, but can not provide strong security assurance. We present a mixed solution by using the trusted computing hardware, namely the time stamping functionality of the trusted platform module, in combination with a timing based remote code integrity verification mechanism. In this way, we do not require a secure operating system, but at the same time the overall security of the timed execution scheme can be improved. 相似文献
9.
No Heading We present vibrating wire viscometer experiments in concentrated and dilute 3He-4He mixtures showing that the slip length may become orders of magnitude larger than the mean free path due to increased specular scattering of the 3He quasiparticles with the superfluid 4He film on the wire. The experimental results are in good agreement with a recent theory for slip which accounts for the cylindrical geometry of the wire and for velocity slip in directions normal as well as tangential to the surface of the wire.PACS numbers: 67.60.Fp, 51.20.+d 相似文献
10.