Fractionation of transgenic corn for recovery of recombinant enzymes

Two germ-separation methods, dry-milling and density separation by flotation, were evaluated for recovering recombinant β-glucuronidase (rGUS) that accumulated primarily in the germ of transgenic corn. The dry-milling process consisted of (i) seed tempering, (ii) degerming with a horizontal-drum degermer/dehuller, (iii) particle size fractionation with standard sieves, (iv) germ and endosperm separation by roller milling and sifting, and (v) removal of hulls by aspiration. Sieves nos. 5, 6, and 7 retained the majority of germ, and subfractions from these sieves were pooled as a germ-rich fraction. Mass balances showed that the germ-rich fraction, which constituted 17% of the total dry-milled corn weight, contained 49% of rGUS activity and 64% of the total recoverable oil. Germ fractionation by flotation was tested as a proof-of-concept method aimed at separating corn fractions based on their difference in specific gravity (sp gr). The process consisted of impact-grinding of corn kernels followed by density separation using 1.15 or 1.3 specific gravity sodium nitrate solution. The oil-containing germ fraction floated, whereas the heavier endosperm fraction sedimented. The flotation method was simpler and resulted in higher enzyme recovery, that is, the germ-rich fraction was 20% (w/w) of the initial corn weight, and accounted for 80% of rGUS activity and 77% of total oil. The sodium nitrate solution did not have an adverse effect on the enzyme activity.     

Noise analysis of robot manipulator using neural networks

Due to a lot of robot manipulators application in industry, low noise degree is very important criteria for robot manipulator's joints. In this paper, joint noise problem of a robot manipulator with five joints is investigated both theoretically and experimentally. The investigation is consisted of two steps. First step is to analyze the noise of joints using a hardware and software. The hardware is a part of noise sensors. The second step; according to experimental results, some neural networks are employed for finding robust neural noise analyzer. Five types of neural networks are used to compare each other. From the results, it is noted that the proposed RBFNN gives the best results for analyzing joint noise of the robot manipulator.     

Approximate periodic solutions for the Helmholtz-Duffing equation

Approximate periodic solutions for the Helmholtz-Duffing oscillator are obtained in this paper. He’s Energy Balance Method (HEBM) and He’s Frequency Amplitude Formulation (HFAF) are adopted as the solution methods. Oscillation natural frequencies are analytically analyzed. Error analysis is carried out and accuracy of the solution methods is evaluated.     

Dielectric properties of sol-gel derived Ta2O5 thin films

The dielectric constant and the dielectric loss of tantalum pentoxide (Ta₂O₅) thin films, produced by sol-gel spin-coated process on Corning glass substrates, have been investigated in the frequency range of 20-10⁵ Hz and the temperature range of 183-403 K, using ohmic Al electrodes. The frequency and temperature dependence of relaxation time has also been determined. The capacitance and loss factor were found to decrease with increasing frequency and increase with increasing temperature. The activation energy values were evaluated and a good agreement between the activation energy values obtained from capacitance and dielectric loss factor measurements were observed.     

Investigation of Al-O-Al sites in an Na-aluminosilicate glass

This paper reports the presence of Al- O- Al linkages in an aluminosilicate glass where Si/Al = 1 by using 2D¹⁷O triple quantum MAS NMR technique (3Q MASNMR). The experiments were performed at external magnetic fields of 8.4 and 14.4T. Despite¹⁷OMAS NMR spectra of the sample in both fields do not give much information about the different kinds of linkages in the sample, 3Q MAS NMR spectrum shows clear evidence that there are some amounts of Al-O-Al linkages in the sample giving two completely resolved peaks. These two peaks were attributed to the Si-O-Al and Al-O-Al linkages on the basis of their chemical shifts and, quadrupolar coupling constants which are quite sensitive to the local structure.     

Coal consumption and industrial production nexus in USA: Cointegration with two unknown structural breaks and causality approaches

The paper investigates the causality relationships among industrial production index, coal consumption and employment in industrial sector for the period of 1973:1–2011:10 in USA. After noticing that there are breaks in the regression model, the Hatemi-J test for cointegration is employed to the cases that take into account two possible regime shifts. It is concluded that there is a long run relationship between industrial production and industrial coal consumption with the breaks at 1983:4 and 1998:4. We found a negative relationship between coal consumption and industrial production for the period of 1973:1–1983:4 and positive relationship for 1983:5–1998:4 period. For the last period that covers 1983:5–2011:10, the cointegration relationship turned to negative. In addition, the results show that causal relationship between coal consumption and industrial production changes over time.     

Investigation of Lu effect on YBa2Cu3O7−δ superconducting compounds

This study reports the effect of Lu addition on the microstructural and superconducting properties of YBa₂Lu_xCu₃O_7?δ (Y123) superconducting samples with x = 0, 0.1, 0.3, 0.5 and 0.7 by means of X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), electron dispersive X-ray (EDX), electrical resistivity and transport critical current density (J_c) measurements. The samples prepared by the liquid ammonium nitrate and derivatives are exposed to various annealing time (20, 40 and 60 h) and temperature (950, 960 and 970 °C), and the best ambient for the sample fabrication is determined to be 970 °C for 20 h. Zero resistivity transition temperatures (T_c), critical current densities (J_c), variation of transition temperatures, hole-carrier concentration, grain size, lattice parameter, surface morphology, element distribution, crystallinity and resistivity (at room temperature) values of the bulk superconducting samples prepared at 970 °C for 20 h are compared with each other. T_c and J_c values of the samples are inferred from the dc resistivity and the critical current measurements, respectively. The results show that the T_c value of the pure sample is about 90.6 K while the sample doped with 0.1 wt% Lu has the maximum T_c value (92.5 K). However, beyond x = 0.1, the T_c value is observed to decrease toward to 83.5 K with increment in the Lu addition. Similarly, the J_c values measured are found to reduce from 142 to 76 A/cm² with the addition. Moreover, XRD measurements show that both pure and Lu-doped samples exhibit the polycrystalline superconducting phase with the changing intensity of diffraction lines and contain Y123 and Y211 phase, confirming the incorporation of Lu atoms into the crystalline structure of the samples studied. At the same time, comparing of the XRD patterns of samples, the intensity ratio of the characteristic (110) and (013) peaks on the sample doped with 0.1 wt% Lu is more than that on the other samples prepared. Additionally, SEM images display that the sample doped with 0.1 wt% Lu obtains the best crystallinity, grain connectivity and largest grain size whereas the worst surface morphology is observed for the maximum doped sample (x = 0.7). Further, EDX results demonstrate that the Lu atoms doped are successfully introduced into the microstructure of the Y123 samples studied and the maximum Cu element level is observed for the sample doped with 0.1 wt%, explaining that why this sample obtains the best superconducting properties compared to others. According to all the results obtained, it is concluded that the 0.1 wt% Lu addition into the Y123 system improves the microstructural and superconducting properties of the samples studied.     

Do ozone and boric acid affect microleakage in class V composite restorations?

The aim of this in vitro study was to evaluate the effects of chlorhexidine gluconate (2%), sodium hypochloride (2.5%), ozone gas, and boric acid at different concentrations (1%, 3%, 5%, and 7%) on microleakage from composite restorations.

In a total of 80 extracted human canine teeth, a class V cavity was opened on the buccal surface and the samples were separated into eight groups. In the control group, no procedure was applied for cavity disinfection, then composite restoration (Z250, 3M) was made using single-stage, self-etch adhesive (Single Bond 3M). In the other groups, seven different disinfectants were used, then the cavity was restored. The teeth were split into two in the buccolingual direction, parallel to the long axes. Stain penetration was examined under stereomicroscope and scored. Examination with SEM was made on one sample from each group, selected at random. Statistical evaluations were made using Dunnett C Post Hoc Comparison and Kruskal–Wallis H tests.

In the occlusal region evaluation, the groups with the lowest level of leakage were the 3% and 5% boric acid groups, and the highest levels of microleakage were determined in the chlorhexidine group and the 1% boric acid group. In the gingival region, the lowest level of microleakage was in the 5% boric acid group and the highest levels were determined in the 1% and 7% boric acid groups.

Boric acid disinfectants used at suitable concentrations were not seen to create a risk in respect of microleakage.