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1.
Zhuolong Tu Mi Chen Min Wang Zhenxuan Shao Xiaoqi Jiang Kangyan Wang Zhe Yao Shiwei Yang Xingxing Zhang Weiyang Gao Cai Lin Bo Lei Cong Mao 《Advanced functional materials》2021,31(30):2100924
Diabetic wound healing still faces great challenges due to the excessive inflammation, easy infection, and impaired angiogenesis in wound beds. The immunoregulation of macrophages polarization toward M2 phenotype that facilitates the transition from inflammation to proliferation phase has been proved to be an effective way to improve diabetic wound healing. Herein, an M2 phenotype-enabled anti-inflammatory, antioxidant, and antibacterial conductive hydrogel scaffolds (GDFE) for producing rapid angiogenesis and diabetic wound repair are reported. The GDFE scaffolds are fabricated facilely through the dynamic crosslinking between polypeptide and polydopamine and graphene oxide. The GDFE scaffolds possess thermosensitivity, self-healing behavior, injectability, broad-spectrum antibacterial activity, antioxidant and anti-inflammatory ability, and electronic conductivity. GDFE effectively activates the polarization of macrophages toward M2 phenotype and significantly promotes the proliferation of dermal fibroblasts, the migration, and in vitro angiogenesis of endothelial cells through paracrine mechanisms. The in vivo results from a full-thickness diabetic wound model demonstrate that GDFE can rapidly promote the diabetic wound repair and skin regeneration, through fast anti-inflammation and angiogenesis and M2 macrophage polarization. This study provides highly efficient strategy for treating diabetic wound repair through designing the M2 polarization-enabled anti-inflammatory, antioxidant, and antibacterial bioactive materials. 相似文献
2.
Xi Zhao Hongyue Li Fangchao Cui Jinxiang Wang Shumin Yi Hongbo Mi Yanfang Lv Xuepeng Li Jianrong Li 《International Journal of Food Science & Technology》2022,57(7):4400-4410
Taking air freezing (AF) as the reference, the effects of four types of multi-compound freezing medium for cryogenic liquid quick-freezing (immersion freezing, IF) on the freezing rate, quality and myofibrillar protein (MP) denaturation of red drum (Sciaenops ocellatus) fillets during frozen storage (−18°C) from days 0 to 90 were studied. Samples were gathered on days 0, 15, 30, 45, 60 and 90 for analysis. The results showed that IF groups (IF-1: 20% ethanol, 30% propylene glycol, 10% sodium chloride aqueous solution; IF-2: 20% ethanol, 20% propylene glycol, 10% glycerol, 10% sodium chloride aqueous solution; IF-3: 20% ethanol, 20% propylene glycol, 10% polyethylene glycol, 10% sodium chloride aqueous solution; IF-4: 20% ethanol, 20% propylene glycol, 5% glycerol, 5% polyethylene glycol, 10% sodium chloride aqueous solution) significantly shortened the time to cross the formation zone of maximum ice crystals while the freezing rate was 6.13 times higher than the AF group after adding 30% propylene glycol as the freezing medium. Furthermore, compared with the AF group, the IF groups significantly reduced losses in the water-holding capacity, the myofibrillar fragmentation index, microstructure damage, texture characteristics, drip loss and water migration (P < 0.05). In addition, the MP of IF groups had higher maximum transition temperatures (Tmax1 and Tmax2), total sulfhydryl content, Ca2+-ATPase activity and relative α-helix content compared with the AF group. In conclusion, IF could significantly increase the freezing rate of red drum fillets, and slow down quality deterioration and denaturation of MP during frozen storage for 90 days (−18°C). In particular, IF-2 (20% ethanol, 20% propylene glycol, 10% glycerol, 10% sodium chloride aqueous solution) was found to be more suitable for the immersion freezing of marine fish among the four multi-compound freezing medium. 相似文献
3.
Amyloid precursor protein (APP) is a type 1 transmembrane glycoprotein, and its homologs amyloid precursor-like protein 1 (APLP1) and amyloid precursor-like protein 2 (APLP2) are highly conserved in mammals. APP and APLP are known to be intimately involved in the pathogenesis and progression of Alzheimer’s disease and to play important roles in neuronal homeostasis and development and neural transmission. APP and APLP are also expressed in non-neuronal tissues and are overexpressed in cancer cells. Furthermore, research indicates they are involved in several cancers. In this review, we examine the biological characteristics of APP-related family members and their roles in cancer. 相似文献
4.
Ting Yu Wen Nie Zehua Hong Yihong He Jing Chen Xue Mi Shuping Yang Xiaoling Li Bilan Wang Yunzhu Lin Xiang Gao 《Advanced functional materials》2021,31(22):2100715
Normalizing the tumor-induced immune deficiency in the immunosuppressive tumor microenvironment (TME) through increasing the efficient infiltration and activation of antitumoral immunity in TME is the core of promising immunotherapy. Herein, a Cyclo(Arg-Gly-Asp-d -Phe-Lys) (RGD) peptides-modified combinatorial immunotherapy system based on the self-assembly of the nanoparticles named RGD-DMA composed of RGD-PEG-PLA, methoxy poly(ethylene glycol)-poly(lactide) (MPEG-PLA) and 1,2-Dioleoyl-3-trimethylammonium-propane (DOTAP) is used to codeliver the immunostimulatory chemokine CCL19-encoding plasmid DNA (CCL19 pDNA) and immune checkpoint ligand PD-L1 inhibitor (BMS-1). The RGD-DMA/pCCL19-BMS-1 system not only exhibited significant inhibition of tumor progression but also induced locally high concentrations of immunostimulatory cytokines at tumor sites without causing an obviously systemic inflammatory response. The immunosuppressive TME is efficaciously reshaped by the coadministration of RGD-DMA/pCCL19 and BMS-1, as indicated by the activated T lymphocytes, increased intratumoral-infiltration of mature dendritic cells (DCs), and the repolarization of macrophages from pro-tumoral M2-phenotype toward tumoricidal M1-phenotype. The upregulated PD-L1 expression at tumor sites caused by the increased IFN-γ levels after immunostimulatory gene therapy further demonstrated the synergistic effects of BMS-1 in counteracting the inhibitory role of PD-L1 expression in antitumor immunity. Therefore, the combination of immunostimulating therapy and immune checkpoint inhibitor that synergistically target multiple immune regulatory pathways demonstrates significant potential as a novel immunotherapy approach. 相似文献
5.
Junyu Chen Yuze Li Yuming Jiang Liucheng Mao Mi Lai Lixia Jiang Huihui Liu Zongxiu Nie 《Advanced functional materials》2021,31(52):2106743
Cancer remains an intractable medical problem. Rapid diagnosis and identification of cancer are critical to differentiate it from nonmalignant diseases. High-throughput biofluid metabolic analysis has potential for cancer diagnosis. Nevertheless, the present metabolite analysis method does not meet the demand for high-throughput screening of diseases. Herein, a high-throughput, cost-effective, and noninvasive urine metabolic profiling method based on TiO2/MXene-assisted laser desorption/ionization mass spectrometry (LDI-MS) is presented for the efficient screening of bladder cancer (BC) and nonmalignant urinary disease. Combined with machine learning, TiO2/MXene-assisted LDI-MS enables high diagnostic accuracy (96.8%) for the classification of patient groups (including 47 BC and 46 ureteral calculus (UC) patients) from healthy controls (113 cases). In addition, BC patients can also be identified from noncancerous UC individuals with an accuracy of 88.3% in the independent test cohort. Furthermore, metabolite variations between BC and UC individuals are investigated based on relative quantification, and related pathways are also discussed. These results suggest that this method, based on urine metabolic patterns, provides a potential tool for rapidly distinguishing urinary diseases and it may pave the way for precision medicine. 相似文献
6.
The Journal of Supercomputing - Dockless bike-sharing is more and more popular all over the world, which enables passengers to choose destinations more flexibly, so that the transfer radius between... 相似文献
7.
目的评估高效液相色谱法测定维生素K胶囊中的维生素K_2含量的不确定度。方法采用高效液相色谱法测定维生素K胶囊中的维生素K_2含量,建立数学模型,对测定过程的主要不确定度分量进行计算,得出合成不确定度和扩展不确定度。结果维生素K胶囊中维生素K_2的含量为181.2μg/g时,在置信区间95%下,其扩展不确定度为7.5μg/g(k=2)。结论不确定度的主要来源为标准曲线配制的过程,该评估模型为维生素K胶囊中维生素K_2的含量测定的准确性提供了参考依据。 相似文献
8.
以攀西钒钛磁铁尾矿和废玻璃为主要原料通过高温烧结法制备储水泡沫陶瓷,研究原料配比和发泡剂(SiC)添加量对材料性能的影响。结果表明:随着钒钛磁铁矿尾矿含量的增加,材料的体积密度及抗压强度逐渐增大,平均气孔孔径逐渐减小;当尾矿添加量为50 wt%,材料的体积吸水率出现极值。当SiC添加量为0.3 wt%,材料内部气孔分布均匀,平均孔径约为2.93 mm。最终以50.0 wt%的钒钛磁铁矿尾矿和50.0 wt%的废玻璃为原料,外加3.0 wt%的石英,0.3 wt%的SiC,3.0 wt%的Na3PO4,在1040℃下制得性能最优的储水泡沫陶瓷,材料的体积密度为0.26 g/cm-3、体积吸水率为56.5%和抗压强度为0.68 MPa。采用SEM、XRD等检测手段研究材料的微观形貌及物相组成,结果表明储水泡沫陶瓷内部由三维立体结构组成,有利于储存水分;材料主要物相包括硅灰石、长石、透辉石和钛铁矿。 相似文献
9.
10.
利用Thermo-Calc软件对镍基耐蚀Hastelloy G3合金进行了热力学计算,系统研究了成分对平衡相析出的影响规律,并通过SEM和TEM对合金时效处理后的析出相进行了观察。结果表明,合金析出的平衡相主要为σ相、μ相、M6C和M23C6;Cr、Mo含量主要影响σ相和μ相析出量及开始析出温度;而C元素显著影响碳化物M6C和M23C6的析出行为;进一步通过试验研究了时效过程中析出相的析出规律。 相似文献