Doxorubicin increases endothelial permeability, hence increasing cardiomyocytes’ exposure to doxorubicin (DOX) and exposing myocytes to more immediate damage. Reactive oxygen species are major effector molecules of doxorubicin’s activity. Mangiferin (MGN) is a xanthone derivative that consists of C-glucosylxanthone with additional antioxidant properties. This particular study assessed the effects of MGN on DOX-induced cytotoxicity in human umbilical vein endothelial cells’ (HUVECs’) signaling networks. Mechanistically, MGN dramatically elevated Nrf2 expression at both the messenger RNA and protein levels through the upregulation of the PI3K/AKT pathway, leading to an increase in Nrf2-downstream genes. Cell apoptosis was assessed with a caspase-3 activity assay, transferase-mediated dUTP-fluorescein nick end labeling (TUNEL) staining was performed to assess DNA fragmentation, and protein expression was determined by Western blot analysis. DOX markedly increased the generation of reactive oxygen species, PARP, caspase-3, and TUNEL-positive cell numbers, but reduced the expression of Bcl-2 and antioxidants’ intracellular concentrations. These were effectively antagonized with MGN (20 μM), which led to HUVECs being protected against DOX-induced apoptosis, partly through the PI3K/AKT-mediated NRF2/HO-1 signaling pathway, which could theoretically protect the vessels from severe DOX toxicity. 相似文献
One of the strategies to overcome the drawbacks of fast charge recombination of a photocatalyst is to develop semiconductor heterostructures. Herein, we report a two-step precipitation-hydrothermal process to create CuFeO2-ZnO heterostructures with different weight percentages of CuFeO2 (0.5, 1, 5, and 10%). Though X-ray diffraction detected the presence of CuFeO2 on ZnO above 5%, Raman spectroscopy could reveal the presence of CuFeO2 phase as low as 0.5 wt%. For all of the compositions, the bandgap of ZnO did not vary (3.15 eV) on forming heterostructures with CuFeO2. The oxidation of methylene blue under sunlight was used to determine the photocatalytic performance of the heterostructures. In comparison to pure ZnO and CuFeO2, CuFeO2-ZnO heterostructures exhibited a better photocatalytic efficiency. Overall, 5 wt% CuFeO2 on ZnO showed 100% degradation with a rate constant of 0.272?±?0.002 min?1, which is 16 times faster than ZnO. Time-resolved photoluminescence analysis indicated a higher lifespan of charge carriers in the 5wt% CuFeO2-ZnO heterostructure (32.3 ns) than that of CuFeO2 (0.85 ns) and ZnO (27.6 ns). The Mott–Schottky flat band potentials of ZnO and CuFeO2 was determined to be -0.82 and 1.17 eV, respectively, revealing the presence of Type I heterostructures. The heterostructures also showed outstanding recyclability, with a degradation rate of 97% even after four cycles. The current study shows the significance of forming p-type CuFeO2 and n-type ZnO heterostructures for enhanced photocatalysis.
Polymer Bulletin - Tri-substituted cardanol based benzoxazine with functionalized rice husk ash (CBz/FRHA) bio-composites were developed using renewable resource materials, and... 相似文献
Defective protein folding and accumulation of misfolded proteins is associated with neurodegenerative, cardiovascular, secretory, and metabolic disorders. Efforts are being made to identify small-molecule modulators or structural-correctors for conformationally destabilized proteins implicated in various protein aggregation diseases. Using a metastable-reporter-based primary screen, we evaluated pharmacological chaperone activity of a diverse class of natural products. We found that a flavonoid glycoside ( C-10 , chrysoeriol-7-O-β-D-glucopyranoside) stabilizes metastable proteins, prevents its aggregation, and remodels the oligomers into protease-sensitive species. Data was corroborated with additional secondary screen with disease-specific pathogenic protein. In vitro and cell-based experiments showed that C-10 inhibits α-synuclein aggregation which is implicated in synucleinopathies-related neurodegeneration. C-10 interferes in its structural transition into β-sheeted fibrils and mitigates α-synuclein aggregation-associated cytotoxic effects. Computational modeling suggests that C-10 binds to unique sites in α-synuclein which may interfere in its aggregation amplification. These findings open an avenue for comprehensive SAR development for flavonoid glycosides as pharmacological chaperones for metastable and aggregation-prone proteins implicated in protein conformational diseases. 相似文献
Silicon - In this study, we present an ambipolar conduction and RF stability performance for a Germanium Source Dual Halo Dual Dielectric Triple Material Surrounding Gate Tunnel FET... 相似文献
The power systems are functioning nearer to their capacity limits to increase the efficiency of the operation. With the prompt progress of smart grid, wide area measurement system (WAMS) is broadly and rapidly deployed in the EPS to understand, fore-cast, or even control the status of power grid stability in real time. Introducing phasor measurement units in addition to its advances have made them technically viable to monitor the stability of the PS using a wide area perception. This paper presents the review of WAMS and reports the various aspects of its operation in a power system. Initially, WAMS features, components, process, and its architecture are briefly described. The latter provides the data sources along with their standards required for WAMS and its applications. Also this paper gives a brief review on WAMPAC and various research openings for the development and implementation of real-time functions. 相似文献
Surveys are mainly conducted to obtain valuable information on some criteria from a specified population. But, the survey results often become biased due to non-response of the subjects under study for highly significant attributes. Such non-ignorable missingness need to be treated and the actual values should be retrieved. Many methods have already been proposed for handling missing values in either discrete or continuous attributes. But, there exists a large gap in handling non-ignorable missing values in datasets with mixed attributes. With the intent of addressing this gap, this paper proposes a methodology called as Bayesian Genetic Algorithm (BAGEL) with hybridized Bayesian and Genetic Algorithm principles. In BAGEL, the initial population is generated using Bayesian model and fitness values of the chromosomes are evaluated using Bayesian principles. BAGEL is implemented in real datasets for imputing both discrete and continuous missing values and the imputation accuracy is observed. The experimental results show the superior performance of BAGEL than other standard imputation techniques. Statistical tests conducted to validate the experimental results also prove that BAGEL outperforms at all missing rates from 5% to 50%. 相似文献