Failed Neuroprotection of Combined Inhibition of L-Type and ASIC1a Calcium Channels with Nimodipine and Amiloride

Effective pharmacological neuroprotection is one of the most desired aims in modern medicine. We postulated that a combination of two clinically used drugs—nimodipine (L-Type voltage-gated calcium channel blocker) and amiloride (acid-sensing ion channel inhibitor)—might act synergistically in an experimental model of ischaemia, targeting the intracellular rise in calcium as a pathway in neuronal cell death. We used organotypic hippocampal slices of mice pups and a well-established regimen of oxygen-glucose deprivation (OGD) to assess a possible neuroprotective effect. Neither nimodipine (at 10 or 20 µM) alone or in combination with amiloride (at 100 µM) showed any amelioration. Dissolved at 2.0 Vol.% dimethyl-sulfoxide (DMSO), the combination of both components even increased cell damage (p = 0.0001), an effect not observed with amiloride alone. We conclude that neither amiloride nor nimodipine do offer neuroprotection in an in vitro ischaemia model. On a technical note, the use of DMSO should be carefully evaluated in neuroprotective experiments, since it possibly alters cell damage.     

A probabilistic model for cleavage fracture with a length scale--parameter estimation and predictions of stationary crack experiments

This study presents a large experimental investigation in the transition temperature region on a modified A508 steel. Tests were carried out on single-edge-notch-bend specimens with three different crack depth over specimen width ratios to capture the strong constraint effect on fracture toughness. Three test temperatures were considered, covering a range of 85 °C. All specimens failed by cleavage fracture prior to ductile tearing. A recently proposed probabilistic model for the cumulative failure by cleavage was applied to the comprehensive sets of experimental data. This modified weakest link model incorporates a length scale, which together with a threshold stress reduce the scatter in predicted toughness distributions as well as introduces a fracture toughness threshold value. Model parameters were estimated by a robust procedure, which is crucial in applications of probabilistic models to real structures. The conformity between predicted and experimental toughness distributions, respectively, were notable at all the test temperatures.     

The Gas-to-Particle Heat Transfer and Hydrodynamics in Spouted Bed Drying of Sawdust

This article presents experimental results for spouted bed drying of sawdust, carried out in a full-scale as well as in a laboratory-scale dryer using air as well as steam as drying media. The aim is to present design parameters for a spouted-bed sawdust dryer that can be used by the industry in designing full-scale dryers. A hydrodynamically stable spouted jet spouted bed was obtained. The heat transfer characteristics of the bed were represented in terms of a volumetric heat transfer coefficient (VHC). When sawdust is dried in a spouted bed, the mean VHC is increasing up to fiber saturation level (20-25% wb) from 40 to 110 W/m³ K. The VHC decreases with the residence time and with an increased static bed height. Gas temperature profiles are also presented for the bottom part of the drying chamber.     

Analysis of molecular determinants in native AMPA receptors

For this study, 194 respondents completed a biographical data sheet, the Templer (1970) Death Anxiety Scale and the Constantinople (1973) Inventory of Psychosocial Development to help assess the relationship among death anxiety, age, and psychosocial maturity. Findings showed that psychosocial maturity was a better predictor of death anxiety than age was. However, both variables were significantly negatively correlated with death anxiety, revealing that as psychosocial maturity and age increase, death anxiety decreases.     

D-lactic acidosis: a treatable encephalopathy in pediatric patients

Isolation of Salmonella from the feces of impounded dogs was carried out to make clear the recent microbiological condition of dogs introduced into our facilities. Salmonella was isolated from 10 out of 283 samples (3.5%). Inparticular, during the first week after introduction, Salmonella was isolated from nine out of 74 dogs (12.2%). The isolation rate during the first week after introduction was significantly higher than that for dogs introduced later. No isolates were detected from dogs reared for more than 3 weeks after introduction. This study indicates that impounded dogs had the highest risk of infecting a person with Salmonella during the first week after introduction into our laboratory animal facilities, and that we need to pay attention to this fact when handling them.     

Simulated hot working,cold working,

An investigation was carried out on the effects of changing the hot-working, cold-working, and annealing parameters on the interaction between A1N precipitation and recrystallization in low- carbon steels containing significant amounts of Al and N. The primary aim was to determine whether laboratory torsion testing can be helpful in analyzing precipitation behavior in these steels. The approach taken was first to establish the baseline annealing behavior of material that was hot- and cold-worked in a mill and then to compare this with the behavior of material in which the hot and cold working were simulated by torsion testing. It was found, first of all, that torsion testing can indeed provide a good simulation of the industrial process of hot and cold working followed by annealing, even to the extent that a process as subtle as the inhibition of recrystallization by A1N precipitation can be accurately reproduced. The results also show that the interaction between precipitation and recrystallization during annealing is significantly influenced by the reheat temperature before hot working, as well as by the cooling rate after hot working.     

Influence of strain rate on interpass softening

Most laboratory simulations of hot rolling involve a scaling down of the strain rate from the much higher industrial levels. This leads to slower softening between each rolling pass, for which corrections must be made. In the present work, torsion testing simulations of “warm” rod rolling were conducted on a Ti-Nb interstitial-free (IF) steel at 840 °C and 770 °C(i.e., in the ferrite range). For this purpose, “strain rate corrected” interpass times were used, in addition to the more familiar corrections for the stress. The results are compared with those obtained from simulations using uncorrected industrial interpass times. At 840 °C, simulations using corrected interpass times led to high levels of softening between the stages of rolling, thus triggering the reinitiation of cycles of dynamic recrystallization. The initially high stress level at the start of these cycles was responsible for the large differences in the pass-to-pass mean flow stress behavior, compared with that observed when using uncorrected industrial interpass times, or continuous deformations. The differences were much less pronounced at 770 °C, where the rate of softening is much slower than at 840 °C. Predictions for softening based on the Avrami equation underestimated the softening observed using the continuous and uncorrected industrial interpass time schedules and overestimated it for the corrected ones. The former is due to the occurrence of recovery, which is not addressed by the Avrami relation, while the latter is due to the precipitation that takes place during the corrected (longer) interpass times. It was also found that simulations using continuous deformations are applicable only if the interpass softening that would be expected using the corrected interpass times does not exceed about 20 pct.