Rapid detection of pork meat freshness by using L-cysteine-modified gold electrode

In this research, a simple and rapid glucose sensor was developed and applied in the detection of meat glucose. The gold electrode was modified using L-cysteine and nanogold solution, and a polyglutamate–glucose oxidase (GOx) complex was prepared and dropped on the modified electrode; the electrostatic binding between the L-cysteine on the electrode surface and the polyglutamate moiety of the complex resulted in the formation of a GOx-attached electrode. Glucose in meat was detected, and the enzyme electrode showed a quick response. The optimum operating conditions for this sensor were determined as follows: 0.1 M citrate buffer pH 5.0 was found to be suitable for this experiment, and the temperature was kept between 25 and 30 °C which is the suitable range for the GOx activity. A linear relationship was found between 0.1 and 0.9 mM/mL. High pressure liquid chromatography (HPLC) was also used to detect pork meat glucose, and total volatile basic nitrogen (TVBN) production was as well detected as a quality index of pork meat freshness. The results showed that the developed sensor was fast and reliable method for the detection of glucose in meat.     

Design of dedicated seven-port star dividers for sectored circular antenna array feeding

In order to mitigate the multipath propagation problems for high rate wlans such as Hiperlan (5 GHz) [2], we study an approach based on electronic beam steering over 360° in azimuth with the help of circular arrays of monopoles [3]. Design simplicity and size constraints intended for potential low-cost applications on the terminals has led us to favor a beam switching topology, using a single feeding-port [4]. Two competing configurations of the microstrip feeding circuit are presented and their principle of operation is experimentally validated through passive circuit configurations. The power distribution is achieved in the first one with the help of a pseudo-divider based on a modified 7-branch star junction, whose specific advantage is its very small size. Unfortunately, this design does not preserve the circular permutation invariance of the radiation patterns. The second circuit bypasses this problem in achieving a power distribution through a virtual point junction, at the cost of an appreciable increase in the circuit size.     

Chromophore‐Linked Substrate (CLS405): Probing Metallo‐β‐Lactamase Activity and Inhibition

Serine‐ and metallo‐β‐lactamases present a threat to the clinical use of nearly all β‐lactam antibiotics, including penicillins, cephalosporins, and carbapenems. Efforts to develop metallo‐β‐lactamase (MBL) inhibitors require suitable screening platforms to allow the rapid determination of β‐lactamase activity and efficient inhibition. Unfortunately, the platforms currently available are not ideal for this purpose. Further progress in MBL inhibitor identification requires inexpensive and widely applicable assays. Herein the identification of an inexpensive and stable chromogenic substrate suitable for use in assays of clinically relevant MBLs is described. (6R,7R)‐3‐((4‐Nitrophenoxy)methyl)‐8‐oxo‐7‐(2‐phenylacetamido)‐5‐thia‐1‐azabicyclo[4.2.0]oct‐2‐ene‐2‐carboxylic acid 5,5‐dioxide (CLS405) was synthesised in a three‐step protocol. CLS405 was then characterised spectroscopically, and its stability and kinetic properties evaluated. With a Δλ_max value of 100 nm between the parent and hydrolysis product, a higher analytical accuracy is possible with CLS405 than with commonly used chromogenic substrates. The use of CLS405 in assays was validated by MBL activity measurements and inhibitor screening that resulted in the identification of N‐hydroxythiazoles as new inhibitor scaffolds for MBLs. Further evaluation of the identified N‐hydroxythiazoles against a panel of clinically relevant MBLs showed that they possess inhibitory activities in the mid‐ to low‐micromolar range. The findings of this study provide both a useful tool compound for further inhibitor identification, and novel scaffolds for the design of improved MBL inhibitors with potential as antibiotics against resistant strains of bacteria.     

Comparison of calibration curve fitting methods in the determination of H2S mass fractions in natural gas by GC-SCD

This paper discusses metrologically the best practice regarding the calibration curves applied to H₂S mass fractions determination in natural gas by gas chromatography with sulfur chemiluminescence detection (GC-SCD). Three calibration curves were constructed by performing GC-SCD analysis of different H₂S gas standard concentrations (from 3 mg kg^?1 up to 500 mg kg^?1). These experimental curves are better fitted by an unweighted quadratic calibration curve considering ANOVA approach compared to ASTM D5504-12. Despite this, the obtained results show that these two different calibration curve approaches (ASTM and ANOVA) lead to comparable results. Hence, there are no significant statistical differences between these two approaches based on the hypothesis test applied. However, the quadratic calibration curve presents measurement uncertainties of H₂S mass fractions much lower than the ASTM approach.     

Isothermal Microcalorimetry to Investigate Non Specific Interactions in Biophysical Chemistry

Isothermal titration microcalorimetry (ITC) is mostly used to investigate the thermodynamics of “specific” host-guest interactions in biology as well as in supramolecular chemistry. The aim of this review is to demonstrate that ITC can also provide useful information about non-specific interactions, like electrostatic or hydrophobic interactions. More attention will be given in the use of ITC to investigate polyelectrolyte-polyelectrolyte (in particular DNA-polycation), polyelectrolyte-protein as well as protein-lipid interactions. We will emphasize that in most cases these “non specific” interactions, as their definition will indicate, are favoured or even driven by an increase in the entropy of the system. The origin of this entropy increase will be discussed for some particular systems. We will also show that in many cases entropy-enthalpy compensation phenomena occur.     

Frequency-Selective Predistortion Linearization of RF Power Amplifiers

This paper presents a frequency-selective RF vector predistortion linearization system for RF multicarrier power amplifiers (PAs) affected by strong differential memory effects. Differential memory effects can be revealed in two-tone experiment by the divergence for increasing tone-spacing of the vector Volterra coefficients associated with the lower and upper intermodulations tones. Using large-signal vector measurement with a large-signal network analyzer, a class-AB LDMOS RF PA is demonstrated to exhibit a strong differential memory effect for modulation bandwidth above 0.3 MHz. New frequency-selective RF and baseband predistortion linearization algorithms are proposed to separately address the linearization requirements of the interband and inband intermodulation products of both the lower and upper sidebands. Theoretical verification of the algorithms are demonstrated with Matlab simulations using a Volterra/Wiener PA model with memory effects. The baseband linearization algorithm is next implemented in a field-programmable gate array and experimentally investigated for the linearization of the class-AB LDMOS PA for two carrier wideband code-division multiple-access signals. The ability of the algorithm to selectively linearize the two interband and four inband intermodulation products is demonstrated. Adjacent channel leakage ratio of up to 45 dBc for inband and interband are demonstrated experimentally at twice the typical fractional bandwidth.     

Variability and molecular typing of Streptococcus thermophilus strains displaying different proteolytic and acidifying properties

Proteolytic and acidifying properties of Streptococcus thermophilus strains isolated from yoghurt or cheeses were evaluated. Among 30 strains tested, 12 exhibited cell envelope-associated proteinase activity (PrtS⁺), three displayed a slight PrtS activity (PrtS^+/?) and 15 were PrtS^?, despite the presence of the corresponding gene (prtS) in eight of them. Sequencing of the prtS gene in four PrtS^? and one PrtS⁺ strains revealed that the absence of PrtS activity in the PrtS^? strain probably results from an alteration of the prtS regulation. The strains displaying the highest acidifying capacities were all PrtS⁺. All but one PrtS⁺ strains were phylogenetically close, as shown by the sequencing of their rDNA internal transcribed spacer (ITS) 16S-23S. More specifically, the high proteolytic and acidifying capacities are associated with the presence of a type II-ITS.     

An Integrated Model for an Industrial Production–Distribution Problem

In this paper, we are interested in a multi-facility, multi-product and multi-period industrial problem. In this problem, both production and distribution costs are significant and they are inter-related. Therefore they should be considered simultaneously in a cost optimization problem. We model this combined production-distribution problem in the form of a network flow problem with relatively few additional 0-1 variables describing the linking constraints between periods. Computational experiments show that the real size problems we encountered can be solved in reasonable time using commercial linear programming codes like CPLEX     

Microbiologic efficacy of azithromycin and susceptibilities to azithromycin of isolates of Chlamydia pneumoniae from adults and children with community-acquired pneumonia

Chlamydia pneumoniae was eradicated from the nasopharynges of 26 of 33 (78.8%) evaluable children and adults with community-acquired pneumonia who were treated with azithromycin. We tested 55 isolates of C. pneumoniae obtained from 46 of these patients against azithromycin. The MIC at which 90% of the isolates were inhibited and the minimal chlamydiacidal concentration at which 90% of strains tested were killed of azithromycin for these isolates were both 0.5 microg/ml. Seven patients remained culture positive after treatment. The MICs of azithromycin for isolates from two patients increased fourfold after therapy. However, all the patients with persistent infection improved clinically. Further studies of treatment of C. pneumoniae infection, utilizing culture, are needed both to assess efficacy and to monitor for the possible development of antibiotic resistance.