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1.
Shinsaku Tokuda Young Hak Kim Hisako Matsumoto Shigeo Muro Toyohiro Hirai Michiaki Mishima Mikio Furuse 《PloS one》2015,10(12)
The relationship between chronic inflammation and cancer is well known. The inflammation increases the permeability of blood vessels and consequently elevates pressure in the interstitial tissues. However, there have been only a few reports on the effects of hydrostatic pressure on cultured cells, and the relationship between elevated hydrostatic pressure and cell properties related to malignant tumors is less well understood. Therefore, we investigated the effects of hydrostatic pressure on the cultured epithelial cells seeded on permeable filters. Surprisingly, hydrostatic pressure from basal to apical side induced epithelial stratification in Madin-Darby canine kidney (MDCK) I and Caco-2 cells, and cavities with microvilli and tight junctions around their surfaces were formed within the multi-layered epithelia. The hydrostatic pressure gradient also promoted cell proliferation, suppressed cell apoptosis, and increased transepithelial ion permeability. The inhibition of protein kinase A (PKA) promoted epithelial stratification by the hydrostatic pressure whereas the activation of PKA led to suppressed epithelial stratification. These results indicate the role of the hydrostatic pressure gradient in the regulation of various epithelial cell functions. The findings in this study may provide clues for the development of a novel strategy for the treatment of the carcinoma. 相似文献
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Preferential ADP-ribosylation of arginine-3 in synthetic heptapeptide Leu-Arg-Arg-Ala-Ser-Leu-Gly. 下载免费PDF全文
R Matsuura Y Tanigawa M Tsuchiya K Mishima Y Yoshimura M Shimoyama 《The Biochemical journal》1988,253(3):923-926
Hen liver nuclear ADP-ribosyltransferase modified the synthetic heptapeptide Kemptide (Leu-Arg-Arg-Ala-Ser-Leu-Gly) at arginine-2 and/or arginine-3. Trypsin treatment of ADP-ribosyl-Kemptide revealed that the ADP-ribosylation of arginine-3 was constantly more abundant than that of arginine-2. ADP-ribosylation of Kemptide suppressed the subsequent phosphorylation by cyclic AMP-dependent protein kinase. 相似文献
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The eggs ofAlcichthys alcicornis were spawned in tank at the laboratory and reared for the studies of embryonic, larval and juvenile development. This species takes place entosomatic fertilization, and females spawn fertilized eggs after copulation. The eggs are demersal and adhesive, released as a clump forming a thin layer on the bottom of tank. There was no significant difference in embryonic development between this species and other oviparous teleostean species. Hatching occurred between 17 and 18 days after spawning at a mean water temperature of 8.5?C. The newly hatched larvae averaged 4.44 mm in body length (BL). The larvae attained to post-larval stage at 5.80 mm BL, and juvenile stage at 10.2 mm BL. A specific feature of the post-larvae was the appearance of three lines of the melanophores on the caudal part of fin fold. Carotenoid first appeared on the nape at 8.70 mm BL, heavily emerged beyond 12.9 mm BL, and turned up on the back also beyond 15.2 mm BL. Scales on the lateral line were completed by 18.5 mm BL. Three pairs of flaps were observed on the dorsal surface of the head at 37.0 mm BL. External features of adult specimens are almost completed by 52.0 mm BL, yet the tip of the first preopercular was not branched but remained simple. 相似文献
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Formation of a parallel-stranded DNA homoduplex by d(GGA) repeat oligonucleotides. 总被引:2,自引:2,他引:0 下载免费PDF全文
The GGA9-H molecules consisting of a double helical stretch followed by a single-stranded 3'-terminal overhang of nine GGA sequence repeats exhibited a gel mobility-shifted band in a concentration-dependent manner, suggestive of the intermolecular complex formation. The position of the shifted band in a gel was almost identical to that of the Y-shaped dimer marker of the same molecular weight that had the two double-helices at one side. This suggests that GGA9-H dimerizes in a parallel orientation without the formation of four-stranded hairpin structure. Since the GGA9-H homoduplex was stably formed at pH 4, 7 and 9, the formation does not require protonation or deprotonation of the N1 position of adenines. Neither does it require the N7 group of guanines responsible for Hoogsteen base pairing from the methylation interference and modification studies. Modification of the N7 group of guanines with dimethyl sulfate (DMS) did not inhibit the association and also the N7 group in the homoduplex was not protected from DMS. On the other hand, the GAA9-H having the G to A base substitution did not show such an association with either GGA9-H or GAA9-H. These results suggest that the homoduplex formation may be due to G.G base pairing through non-Hoogsteen hydrogen bonds. 相似文献
7.
Renzo Hirayama Kazuto Sato Katsuiku Hirokawa Mei-Ping Chang Yoshio Mishima Takashi Makinodan 《Cancer immunology, immunotherapy : CII》1984,18(3):209-214
Summary An attempt has been made to determine (a) whether aging plays an important role in resistance against metastasis and (b) whether dithiothreitol, an effective in vitro mitogenic potentiator of splenic cells of young and old mice, can modulate the occurrence of pulmonary metastasis. B16-F10 melanoma cells were injected into the outer ear of young and old female C57BL/6 mice; and the growth of the primary tumor, the palpable size of the cervical lymph node, and the number of lung metastases were then determined at various intervals. The ear was amputated when the primary tumor reached 4 mm in mean diameter. The following results were obtained. (a) The growth rate of the primary tumor in young mice is comparable to that in old mice. (b) Enlargement of the cervical lymph node occurs earlier in old than in young mice. (c) Old mice are more vulnerable to pulmonary metastases, but small metastasized pulmonary colonies are more prominent in old than in young mice. (d) Dithiothreitol (100 g) injected every 2 days after the inoculation of tumor cells is effective in reducing the incidence of pulmonary metastases in old mice. 相似文献
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T Horikawa K Nishino Y Mishima 《Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society》1991,4(1):48-51
Normal human skin is shown to contain melanocyte growth factor (MeGF). We found MeGF activity in extracts of both the epidermal portion of skin and the dermal portion. This activity was completely adsorbed onto heparin beads and eluted by 2.5 M NaCl. In addition, the activity of both extracts was completely blocked by antibodies directed against basic fibroblast growth factor (bFGF). It is suggested that melanocytes in epidermis are supported by bFGF-like MeGF in normal human skin. 相似文献