全文获取类型
收费全文 | 229篇 |
免费 | 15篇 |
学科分类
生物科学 | 244篇 |
出版年
2023年 | 1篇 |
2021年 | 2篇 |
2020年 | 6篇 |
2019年 | 9篇 |
2018年 | 1篇 |
2017年 | 9篇 |
2016年 | 8篇 |
2015年 | 10篇 |
2014年 | 18篇 |
2013年 | 8篇 |
2012年 | 24篇 |
2011年 | 14篇 |
2010年 | 7篇 |
2009年 | 9篇 |
2008年 | 12篇 |
2007年 | 12篇 |
2006年 | 14篇 |
2005年 | 21篇 |
2004年 | 9篇 |
2003年 | 15篇 |
2002年 | 7篇 |
2001年 | 3篇 |
2000年 | 7篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1991年 | 2篇 |
排序方式: 共有244条查询结果,搜索用时 15 毫秒
1.
Sam Mavandadi Steve Feng Frank Yu Stoyan Dimitrov Karin Nielsen-Saines William R. Prescott Aydogan Ozcan 《PloS one》2012,7(10)
We propose a methodology for digitally fusing diagnostic decisions made by multiple medical experts in order to improve accuracy of diagnosis. Toward this goal, we report an experimental study involving nine experts, where each one was given more than 8,000 digital microscopic images of individual human red blood cells and asked to identify malaria infected cells. The results of this experiment reveal that even highly trained medical experts are not always self-consistent in their diagnostic decisions and that there exists a fair level of disagreement among experts, even for binary decisions (i.e., infected vs. uninfected). To tackle this general medical diagnosis problem, we propose a probabilistic algorithm to fuse the decisions made by trained medical experts to robustly achieve higher levels of accuracy when compared to individual experts making such decisions. By modelling the decisions of experts as a three component mixture model and solving for the underlying parameters using the Expectation Maximisation algorithm, we demonstrate the efficacy of our approach which significantly improves the overall diagnostic accuracy of malaria infected cells. Additionally, we present a mathematical framework for performing ‘slide-level’ diagnosis by using individual ‘cell-level’ diagnosis data, shedding more light on the statistical rules that should govern the routine practice in examination of e.g., thin blood smear samples. This framework could be generalized for various other tele-pathology needs, and can be used by trained experts within an efficient tele-medicine platform. 相似文献
2.
Manikandan Subramanian Lale Ozcan Devram Sampat Ghorpade Anthony W. Ferrante Jr. Ira Tabas 《PloS one》2015,10(8)
Obesity-induced inflammation in visceral adipose tissue (VAT) is a major contributor to insulin resistance and type 2 diabetes. Whereas innate immune cells, notably macrophages, contribute to visceral adipose tissue (VAT) inflammation and insulin resistance, the role of adaptive immunity is less well defined. To address this critical gap, we used a model in which endogenous activation of T cells was suppressed in obese mice by blocking MyD88-mediated maturation of CD11c+ antigen-presenting cells. VAT CD11c+ cells from Cd11cCre
+
Myd88
fl/fl vs. control Myd88
fl/fl mice were defective in activating T cells in vitro, and VAT T and B cell activation was markedly reduced in Cd11cCre
+
Myd88
fl/fl obese mice. However, neither macrophage-mediated VAT inflammation nor systemic inflammation were altered in Cd11cCre
+
Myd88
fl/fl mice, thereby enabling a focused analysis on adaptive immunity. Unexpectedly, fasting blood glucose, plasma insulin, and the glucose response to glucose and insulin were completely unaltered in Cd11cCre
+
Myd88
fl/fl
vs. control obese mice. Thus, CD11c+ cells activate VAT T and B cells in obese mice, but suppression of this process does not have a discernible effect on macrophage-mediated VAT inflammation or systemic glucose homeostasis. 相似文献
3.
4.
This study concerns the evaluation of leaf anatomical characteristics of the closely related genera Matricaria (4 taxa) and Tripleurospermum (14 endemic taxa, 15 accessions) in Turkey using cluster analysis (CA) and principal component analysis (PCA). All taxa have the same basic leaf structure composed of lower and upper epidermis including anomocytic or anomocytic-anisocytic stomata, parenchymatic mesophyll, and vascular bundle. Statistically significant differences based on ploidy level have been found without generic delimitation. The differences mainly concern stomatal length and the relative size of the vascular bundle. These anatomical characters can be used to distinguish diploid from polyploid taxa of the genera. 相似文献
5.
BODIPY is an important fluorophores due to its enhanced photophysical and chemical properties including outstanding thermal/photochemical stability, intense absorption/emission profiles, high photoluminescence quantum yield, and small Stokes' shifts. In addition to BODIPY, indole and its derivatives have recently gained attention because of their structural properties and particularly biological importance, therefore these molecules have been widely used in sensing and biosensing applications. Here, we focus on recent studies that reported the incorporation of indole‐based BODIPY molecules as reporter molecules in sensing systems. We highlight the rationale for developing such systems and evaluate detection limits of the developed sensing platforms. Furthermore, we also review the application of indole‐based BODIPY molecules in bioimaging studies. This article includes the evaluation of indole‐based BODIPYs from synthesis to characterization and a comparison of the advantages and disadvantages of developed reporter systems, making it instructive for researchers in various disciplines for the design and development of similar systems. 相似文献
6.
Kilercioglu Serdar Ay Ozcan Oksuz Hale Yilmaz Mehmet Bertan 《Molecular biology reports》2020,47(7):5243-5251
Molecular Biology Reports - Emamectin, a neurotoxic agent, is a semi-synthetic insecticide that belongs to the Avermectin family and is used against helmintic infections in the Salmonidae family.... 相似文献
7.
Huseyin Inceer Deniz Guney Sema Hayirlioglu-Ayaz Melahat Ozcan Ibrahim Turna Ali Omer Ucler 《Biologia》2009,64(6):1076-1084
Karyological studies have been investigated within 8 native Anatolian populations of oriental spruce (Picea orientalis (L.) Link) in Turkey. The somatic chromosome number of 2n = 2x = 24 has been observed in all accessions. The karyotypes are generally asymmetrical with most of the chromosomes having median
to median-submedian centromeres. Inter-population variability of the karyotype was summarized with cluster analysis. We found
that the karyotypes have positively correlated with the altitudes of the natural habitats. The high value of karyotype asymmetry
may be attributed to both microenvironment and natural regeneration methods used in oriental spruce. 相似文献
8.
Abstract Porcine pancreatic lipase (PPL), Candida rugosa lipase (CRL), and Castor bean lipase (CBL) were immobilized on celite by deposition from aqueous solution by the addition of hexane. Lipolytic performance of free and immobilized lipases were compared and optimizations of lipolytic enzymatic reactions conditions were performed by free and immobilized derivatives using olive oil as substrate. Afterwards, the influence on lipolysis of castor oil of free lipases and immobilized lipase derivatives have been studied in the case of production of ricinoleic acid. All of the lipases performances were compared and enzyme derivative was selected to be very effective on the production of ricinoleic acid by lipolysis reaction. Various reaction parameters affecting the production of ricinoleic acid were investigated with selected the enzyme derivative. The maximum ricinoleic acid yield was observed at pH 7–8, 50°C, for 3 hours of reaction period with immobilized 1,3-specific PPL on celite. The kinetic constants Km and Vmax were calculated as 1.6 × 10?4 mM and 22.2 mM from a Lineweaver–Burk plot with the same enzyme derivative. To investigate the operational stability of the lipase, the three step lipolysis process was repeated by transferring the immobilized lipase to a substrate mixture. As a result, the percentange of conversion after usage decreased markedly. 相似文献
9.
10.