Prognostic value of aldo-keto reductase family 1 member B10 (AKR1B10) in digestive system cancers: A meta-analysis

Background:Numbers of studies have reported that the expression of aldo-keto reductase family 1 member B10 (AKR1B10) is abnormal in digestive system cancers, and could be used as a prognostic biomarker. However, the results are argued. Therefore, we conduct a meta-analysis to comprehensively evaluate the prognostic value of high AKR1B10 expression for overall survival (OS), disease specific survival (DSS), and disease-free survival/recurrence-free survival (DFS/PFS) in digestive system cancers.Methods:Hazard ratios (HRs) with its 95% confidence intervals (CIs) were calculated to assess the prognostic value of AKR1B10 by using the random effects model. The STATA version 12.0 software were used to perform all the analyses.Results:Eleven articles including 1428 patients involved in this meta-analysis. The pooled analysis suggested that high AKR1B10 expression was not associated with OS (HR: 1.18; 95% CI: 0.69–2.00) and DFS/PFS (HR: 1.08, 95% CI: 0.67–1.76) in digestive system cancers. However, Further analysis revealed that high AKR1B10 expression indicated poor OS in oral squamous cell carcinomas (OSCC) (HR: 2.92, 95% CI: 1.86–4.58) and favorable DSS in hepatocellular carcinoma (HCC) (HR: 0.71, 95% CI: 0.52–0.97).Conclusions:The prognostic value of high AKR1B10 expression varied in different types of digestive system cancers. Further studies exploring the prognostic role of AKR1B10 in digestive system cancers are needed.     

鼻咽部纤维血管瘤血管造影诊断和栓塞治疗的临床研究

目的探讨血管造影诊断和血管栓塞治疗鼻咽部纤维血管瘤的安全性和有效性。方法采用股动脉经路，对32例鼻咽部纤维血管瘤患者进行双侧颈内外动脉造影；并采取超选择插管对鼻咽部纤维血管瘤的供血血管进行栓塞。结果32例经造影诊断明确，且供血血管显示清楚的患者进行了超选择性动脉血管栓塞。栓塞疗法止血效果肯定，术后血管瘤体积明显缩小，术中出血少。结论血管造影和血管栓塞治疗鼻咽部血管瘤是一种安全有效的方法。     

Effect of personality on oral health–related quality of life in undergraduates

Objectives:To evaluate the effects of personality traits and other variables on oral health–related quality of life (OHRQoL) of Chinese undergraduates aged 17 to 24 years with no history of orthodontic treatment.Materials and Methods:A total of 443 undergraduate students aged 17 to 24 years were selected and completed a two-section questionnaire: the Eysenck Personality Questionnaire Short Scale Chinese version and the Oral Health Impact Profile–14 Chinese version (OHIP-14). Malocclusion severity was assessed via clinical examination using the dental health component (DHC) and esthetic component of the Index of Orthodontic Treatment Need (IOTN). Severe dentofacial deformities that required orthognathic surgery were excluded.Results:The total and all dichotomized OHIP-14 dimensions correlated closely with the neuroticism personality traits (P < .001). The total and some subscales of OHIP-14 were related with psychoticism. Women had higher OHIP-14 scores than men, especially in the normal occlusion group. Nevertheless, men with more severe malocclusions had higher scores that were similar to those of women. OHRQoL was significantly different between the major of dentistry and other majors.Conclusions:Personality affects comprehensive OHRQoL, and the most significant predictor is neuroticism. Female young adults have worse OHRQoL than male young adults, especially among those with mild malocclusion.     

精神分裂症与心境障碍患者精神残疾状况对照研究

目的 探讨精神分裂症与心境障碍所致精神残疾患者的临床特征.方法抽取在我院进行精神残疾评定的97例精神分裂症患者设为精神分裂症组,97例心境障碍患者设为心境障碍组,采用世界卫生组织残疾评定量表Ⅱ评定精神残疾状况.结果 精神分裂症组单身、病前有个性缺陷、丧失劳动能力、1级残疾比例及世界卫生组织残疾评定量表Ⅱ总分、理解与交流、与他人相处、社会参与领域评分均显著高于心境障碍组(P＜0.05或0.01);受教育年限、病程均显著低于心境障碍组(P＜0.01).结论精神分裂症与心境障碍均是导致患者出现不同程度精神残疾的重性精神疾病,两者致残的病程及残疾程度等有所不同,但均应作为高危人群进行重点防控.     

OCT与ERG在遗传性视网膜劈裂症临床诊断中的应用价值分析

目的 分析光学相干断层扫描(O.CT)与视网膜电流图(ERG)在遗传性视网膜劈裂症临床诊断中的应用价值.方法 选择我院2017年4月至2021年4月收治的80例疑似遗传性视网膜劈裂症患者,均行O.CT与ERG检查.以荧光素眼底血管造影为金标准,比较O.CT、ERG对遗传性视网膜劈裂症的诊断效能.结果 80例疑似遗传性视...     

工作记忆训练对脑卒中患者执行功能及日常生活活动的影响

目的:观察工作记忆训练对脑卒中患者执行功能及日常生活活动能力的影响。方法:采用随机数字表法将46例脑卒中患者分为观察组及对照组,每组23例。2组患者均常规给予认知康复训练(包括计算机辅助认知训练及上肢机器人虚拟情境训练),观察组患者在此基础上辅以工作记忆训练(主要包括活动记忆训练和回忆任务训练)。于治疗前、治疗4周及8...     

身体活动对海马体可塑性和认知功能的影响

背景:身体活动作为一种实用模型,可以从各个方面来研究运动与大脑健康之间的关联,从而更清楚地认识运动对大脑的促进作用,特别是身体活动对海马体结构和功能的改变。目的:综述身体活动与海马体和认知方面的研究,并提出目前研究和实际应用方面许多亟待解决的问题。方法:以"Physical activity,Exercise,Hippocampal,Cognition,Neuroplasticity,Adaptive,Mechanisms"为检索词,检索PubMed数据库1995至2019年发表的相关文章,文献检索语种限制为英文。纳入身体活动和海马、认知调节机制和应用方面的相关内容。结果与结论:计算机初检得到142篇文献,排除无关和重复的文献,保留89篇进行综述。海马体齿状回具有神经再生的能力。规律性身体活动对身体和大脑健康产生深远的影响,运动可促进海马体齿状回的神经再生,可增加两三倍,此类神经主要发生在齿状回背侧。此外,运动对神经元的成熟、形态和连接性等特性的改变也十分重要,并能改变新神经元的整合通路,增加传入新生神经元和传入细胞突触的数量。海马体齿状回的神经再生与运动诱导的多种因素有关,新生神经元的发育和整合需要多个神经递质的参与,而运动可通过调节兴奋性和抑制性神经递质,促进海马体突触可塑性的变化;运动诱导的血清脑源性神经营养因子水平升高则可减缓海马体体积的变化;运动的持续时间和强度可差异性地调节脑血流量,进而影响神经元活动,而长期运动后血管内皮生长因子的过表达,则可促进海马体的血管增殖,增加海马体的神经发生。此外,运动也可改变成人新生神经元的突触可塑性和连接网络,增强成人新生神经元与现有海马-内嗅通路的整合。     

含B7-H1 shRNA基因的慢病毒表达载体的制备及其抑制效果鉴定

目的 设计针对B7-H1的shRNA序列,构建慢病毒表达载体,包装成病毒颗粒后检测其对U251细胞中B7-H1基因的沉默效果.方法 设计3对针对B7-H1 mRNA的shRNA,化学合成正义链和反义链,退火后与酶切后的pLKO.1载体进行连接.测序正确后包装成病毒并感染U251细胞,分别用qRT-PCR及Western blot法检测干扰效果.结果 qRT-PCR及Western blot结果证实设计的3条RNA干扰序列中有2条可有效沉默U251细胞中B7-H1的表达.结论 所制备的针对B7-H1的shRNA慢病毒能特异性沉默B7-H1.     

鼠胚胎干细胞诱导分化内皮样细胞

目的 采用简单贴壁培养方法诱导胚胎干细胞(embryonic stem cell,ESC)分化为内皮样细胞,为组织工程血管及细胞替代治疗提供新的种子细胞.方法 小鼠ESC株SV129按2×104个/cm2密度传代培养,采用ESC培养基添加1 000 U/mL白血病抑制因子(leukaemia inhibitory factor,LIF)维持其未分化状态.撤除LIF,ESC悬浮培养形成拟胚体(embryoid body,EB),将培养第4天的EB置于含VEGF的培养基中贴壁培养,诱导分化.采用免疫组织化学染色、流式细胞仪分析、DiI标记的乙酰化低密度脂蛋白(1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-labeledacetylated low density lipoprotein,DiI-Ac-LDL)摄取实验以及透射电镜检查鉴定分化细胞的性质.结果 分化产生的内皮样细胞具有内皮细胞形态特征,能摄取DiI-Ac-LDL.ESC诱导分化培养产生的第15代细胞免疫组织化学染色呈Flk-1和CD31阳性;流式细胞仪检测ESC传至9代后CD31阳性细胞>90%;透射电镜可见怀布尔-帕拉德体及内皮细胞间紧密连接.结论 采用简单贴壁培养的方法,单独使用VEGF即可诱导ESC分化为内皮样细胞.诱导培养方法操作简便,经济实用,可为组织工程血管及细胞替代治疗提供所需内皮样细胞.