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排序方式: 共有182条查询结果,搜索用时 15 毫秒
1.
Emna Ouni Sbastien Pyr dit Ruys Marie-Madeleine Dolmans Gaëtan Herinckx Didier Vertommen Christiani A. Amorim 《International journal of molecular sciences》2020,21(23)
Currently, the extracellular matrix (ECM) is considered a pivotal complex meshwork of macromolecules playing a plethora of biomolecular functions in health and disease beyond its commonly known mechanical role. Only by unraveling its composition can we leverage related tissue engineering and pharmacological efforts. Nevertheless, its unbiased proteomic identification still encounters some limitations mainly due to partial ECM enrichment by precipitation, sequential fractionation using unfriendly-mass spectrometry (MS) detergents, and resuspension with harsh reagents that need to be entirely removed prior to analysis. These methods can be technically challenging and labor-intensive, which affects the reproducibility of ECM identification and induces protein loss. Here, we present a simple new method applicable to tissue fragments of 10 mg and more. The technique has been validated on human ovarian tissue and involves a standardized procedure for sample processing with an MS-compatible detergent and combined centrifugation. This two-step protocol eliminates the need for laborious sample clarification and divides our samples into 2 fractions, soluble and insoluble, successively enriched with matrisome-associated (ECM-interacting) and core matrisome (structural ECM) proteins. 相似文献
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Brigitte Poulin Aurélie Davranche Gaëtan Lefebvre 《Remote sensing of environment》2010,114(7):1602-2401
Ecologists and conservationists need accurate and replicable tools for monitoring wetland conditions in order to develop and implement adaptive management strategies efficiently. The Rhone Delta (Camargue) in southern France encloses 9200 ha of fragmented reed marshes actively managed for reed harvesting, waterfowl hunting or cattle grazing, and holding significant numbers of vulnerable European birds. We used multi-season SPOT-5 data in conjunction with ground survey to assess the predictive power of satellite imagery in modelling indicators of reed structure (height, diameter, density and cover of green/dry stems) relevant to ecosystem management and bird ecology. All indicators could be predicted accurately with a combination of bands (SWIR, NIR) and indices (SAVI, OSAVI, NDWI, DVI, DVW, MSI) issued from scenes of March, June, July, September or December and subtraction between these. All models were robust when validated with an independent set of satellite and field data. The high spatial resolution of SPOT-5 scenes (pixel of 10 × 10 m) permits the monitoring of detailed attributes characterizing the reed ecosystem across a large spatial extent, providing a scientifically-based, replicable tool for managers, stakeholders and decision-makers to follow wetland conditions in the short and long-term. Combined with models on the ecological requirements of vulnerable bird species, these tools can provide maps of potential species ranges at spatial extents that are relevant to ecosystem functioning and bird populations. 相似文献
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Fatih Çalışır Muhammet Baştan Özgür Ulusoy Uğur Güdükbay 《Multimedia Tools and Applications》2017,76(10):12433-12456
High user interaction capability of mobile devices can help improve the accuracy of mobile visual search systems. At query time, it is possible to capture multiple views of an object from different viewing angles and at different scales with the mobile device camera to obtain richer information about the object compared to a single view and hence return more accurate results. Motivated by this, we propose a new multi-view visual query model on multi-view object image databases for mobile visual search. Multi-view images of objects acquired by the mobile clients are processed and local features are sent to a server, which combines the query image representations with early/late fusion methods and returns the query results. We performed a comprehensive analysis of early and late fusion approaches using various similarity functions, on an existing single view and a new multi-view object image database. The experimental results show that multi-view search provides significantly better retrieval accuracy compared to traditional single view search. 相似文献
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Gaëtan Compère Jean‐François Remacle Johan Jansson Johan Hoffman 《International journal for numerical methods in engineering》2010,82(7):843-867
In this paper, we identify and propose solutions for several issues encountered when designing a mesh adaptation package, such as mesh‐to‐mesh projections and mesh database design, and we describe an algorithm to integrate a mesh adaptation procedure in a physics solver. The open‐source MAdLib package is presented as an example of such a mesh adaptation library. A new technique combining global node repositioning and mesh optimization in order to perform arbitrarily large deformations is also proposed. We then present several test cases to evaluate the performances of the proposed techniques and to show their applicability to fluid–structure interaction problems with arbitrarily large deformations. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
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Development, deployment and application of Information and Communication Technology (ICT) and digital infrastructure continue with unabated intensity in the petroleum-related activity on the Norwegian shelf. This development towards what is denoted Integrated Operations (IO) creates new ways of organizing work, new work processes and increased automation, e.g. closer collaboration offshore-onshore, cooperation across organizational and geographical borders. This creates new challenges for managing risk. Although there are different versions of IO today, we argue that it is possible to identify and study generic properties within such IO manifestations. The current paper focus on the potential complexity of IO in the generic sense, and some scientific implications in terms of addressing risk. The paper uses the century-old metaphor of “wildness in wait” to engage the wide field of complexity theory in a productive way to address systemic properties of risk. The paper further uses the Cynefin sensemaking framework in order to identify and address the crucial distinction between directed (resultant) and un-directed (emergent) order. The paper finally discusses the importance of seeing risk assessment as a social knowledge practice. 相似文献
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以小麦淀粉为原料 ,采用麦芽三糖淀粉酶AMT 1 .2L酶解制备麦芽三糖糖浆 .正交实验确定最佳糖化条件为淀粉乳浓度 2 5 % ,温度为 5 5℃ ,AMT 1 .2L加酶量 5u/g ,初始DE值 8,酶解 2 4h ,可以得到麦芽三糖比例在 70 %以上 ,转化率在 68%以上的糖浆 .用活性炭柱对糖化液进行分离提纯 .核磁共振图谱证实其结构为 3个葡萄糖残基以α 1 ,4键相连接 . 相似文献
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随机断续高频雷达波形设计和处理 总被引:9,自引:1,他引:9
本文综合分析了几种典型高频地波超视距雷达波形的特点,针对这些波形在高频段电磁干扰严重条件下可用连续频带较窄的问题,提出了一种依据外部电磁环境监测结果来截断干扰频段的波形.这种波形能够有效地利用不连续频段,从而提高雷达的有效带宽和抗干扰能力.针对波形频谱截断后引起的距离旁瓣过高问题,本文采用一种改进的自适应目标回波重建算法来处理,达到较好的结果. 相似文献