霍乱弧菌单克隆抗体诊断应用的研究——Ⅱ.酶标霍乱弧菌单克隆抗体快速检测霍乱弧菌的实验研究

本文就所获得的12株霍乱弧菌单克隆抗体中有种特异性的三株(2B_1H_1C_6,2B_1H_1F_6及2B_1H_1F_3)单克隆抗体及抗霍乱弧菌多克隆抗体,作酶标抗体直接法和间接法,对霍乱弧菌模拟标本进行快速检测,探究在临床诊断应用中的价值。结果表明,酶标McAb较酶标多克隆抗体敏感,前者在10~4/ml菌时可以检出,后者要在10~4～10~6/ml菌时才能检出;同时表明酶标间接法比直法敏感,特异性实验结果显示酶标单克隆抗体对10株霍乱弧菌有很高的     

霍乱弧菌单克隆抗体诊断应用的研究——Ⅳ.McAb吖啶橙(McAb-AO)简易免疫荧光菌团法快速检定霍乱弧菌的实验研究

将获得的12株霍乱弧菌单克隆抗体中的一株“2B_1H_1C_6”的McAb与荧光素吖啶橙标记制成简易AOFA、对霍乱弧菌模拟标本进行快速检测,研究其敏感性,特异性和实用性同时以霍乱弧菌多克隆抗体比较。结果表明:McAb-AO比PcAb-AO敏感度高,特异性强(前者仅对霍乱弧菌呈现荧光菌团,对其它肠道菌无交叉反应),在每毫升类模拟标本中含10个     

Acute effects of using a mobile phone on CNS functions

Twenty volunteers participated in two experiments exploring the acute effects of using the mobile phone Motorola GSM 8700 on the functions of the CNS. When speaking (5 minutes reading a text from daily newspapers) the electromagnetic fields from the mobile apparatus did not affect the visual evoked potentials. Also a 6-min exposure did not reveal any effect of electromagnetic fields on the results in two tests (memory and attention) performed while speaking into the mobile. On the other hand the phone call itself strongly influenced the performance in a secondary task applying a test of switching attention which is a good model for driving a car. The response and decision speed were significantly worse. This is a proof that even a slight psychological stress involved in calling while driving can be a great risk.     

Interaction of intravenous anesthetics with human neuronal potassium currents in relation to clinical concentrations

BACKGROUND: Neuronal voltage-dependent potassium (K) currents are crucial for various cellular functions, such as the integration of temporal information in the central nervous system. Data for the effects of intravenous anesthetics on human neuronal K currents are limited. It was the authors' aim to evaluate the concentration-related effects of three opioids (fentanyl, alfentanil, sufentanil) and seven nonopioids (thiopental, pentobarbital, methohexital, propofol, ketamine, midazolam, droperidol) used in clinical anesthesia on neuronal voltage-dependent K currents of human origin. METHOD: K currents were measured in SH-SY5Y cells using the whole cell patch-clamp technique. Currents were elicited by step depolarization from a holding potential of -80 to -50 mV through +90 mV, and their steady state amplitudes were determined. RESULTS: All drugs inhibited the K currents in a concentration-dependent and reversible manner. Because time dependence of inhibition differed among the drugs, effects were measured after 54-64 ms of the test pulse. The IC50 values (concentration of half-maximal inhibition) for current suppression ranged from 7 microM for sufentanil to 2 mM for pentobarbital. Suppression of the K currents by the opioids occurred at 10-fold lower IC50 values (concentration of half-maximal inhibition) than that by the barbiturates. As estimated from the concentration-response curves, K-current suppression at clinical concentrations would be less than 0.1% for the opioids and approximately 3% for the other drugs. CONCLUSIONS: Effects of intravenous anesthetics on voltage-dependent K currents occur at clinical concentrations. The IC50 values for current inhibition of the nonopioid anesthetics correlated with these concentrations (r = 0.95). The results suggest that anesthetic drug action on voltage-dependent K currents may contribute to clinical effects or side effects of intravenous anesthetics.     

Diagnostic imaging in femur head necrosis

Diagnosis of avascular necrosis (AVN) of the hip has been improved by the technical progress of imaging modalities during the last decade. For a long period, only plain radiographs had been available. Scintigraphy and computed tomography contributed to differential diagnosis and early detection of bone necrosis. In the meantime, MR imaging has gained special value in the evaluation of AVN. It is now the method of choice for early detection as well as for assessment in later stage disorders. Using the ARCO system, all imaging modalities and their diagnostic viability are described. Findings regarding the different stages of AVN are correlated to tissue-specific changes.     

Postoperative prophylactic administration of beta-adrenergic blockers in patients at risk for myocardial ischemia

Perioperative myocardial ischemia (MI) is associated with postoperative cardiac morbidity. Postoperative sympatholysis may reduce the incidence of MI. This study evaluated such a reduction postoperatively with the administration of prophylactic beta-blockers in patients undergoing elective total knee arthroplasty with epidural anesthesia and postoperative epidural analgesia. One hundred seven patients were preoperatively randomized into two groups, control and beta-blockers, who received postoperative esmolol infusions on the day of surgery and metoprolol for the next 48 h to maintain a heart rate less than 80 bpm. Patients were followed for ST segment depression by using a Holter monitor and adverse cardiac outcomes. Postoperative electrocardiographic ischemia was significantly more prevalent in the control group compared with the beta-blocker group during esmolol blockade (0 of 52 vs 4 of 55; P = 0.04) and tended to be more common in the control group the next two days (8 of 55 vs 3 of 52; P = 0.135). In addition, the number of ischemic events (control, 50; beta-blockers, 16) and total ischemic time (control, 709 min; beta-blocker, 236 min) were also significantly different from the control group. Myocardial infarctions and cardiac events were more common in the control group, but these differences were not significant. Our results suggest that the use of prophylactic beta-blocker therapy may reduce the incidence of postoperative MI. Implications: Prophylactic beta adrenergic blockade administered after elective total knee arthroplasty was associated with a reduced prevalence and duration of postoperative myocardial ischemia detected with Holter monitoring.     

Vertebral body stenting. A method for repositioning and augmenting vertebral compression fractures

Purpose of the study was to demonstrate the effectiveness of expanding a fractured vertebral body by transpedicular dilatation and stenting. 7 human cadaveric vertebral bodies from L2 to L5 underwent axia compression until a vertebral burst fracture was provoked. Then, by bilateral transpedicular approach, balloon-catheters were introduced, which were armed with stents, usually used for angioplasty. The catheters were inflated with radiolucent fluid and the stents expanded under radiologic control. After expansion, the balloon was deflated and removed, the stents resting inside the vertebral body, holding their inflated shape. Then, the resulting hole was filled with an injectable biodegradable calcium-phosphate. CT-scans were performed after destruction and after expansion. Morphology before and after expansion was judged, using 3-D reconstructions. Vertebral body strength was measured before destruction and after treatment with an Instron testing machine. RESULTS: Vertebral body shape could be restored. Also impressed central parts of the bony endplate could be elevated by using a convergent approach through the pedicles. There was no collapse of the vertebral body after removing the catheter-balloons The vertebral body strength could be restored up to a physiologic level. This procedure gives new perspectives in the treatment either of osteoporotic compression or traumatic vertebral fracture. By using CT-guided technique, it could be performed by a minimally invasive approach percutaneously.     

重组人内皮细胞抑制素腺病毒载体的构建及其应用

目的　构建表达人内皮细胞抑制素 (Endostatin)的重组腺病毒载体 ,并进行活性检测。方法　将含有IL3分泌肽的人Endostatin全长cDNA插入穿梭质粒pAdCMV产生重组质粒pAd IL3 Endo ,通过脂质体介导与 pBHG1 0共转染 2 93细胞 ,经同源重组产生重组腺病毒Ad IL3 Endo。体外转染人结肠癌SW62 0细胞并观察其上清对人脐静脉内皮细胞 (HUVEC)生长的影响。结果　扩增到的Ad IL3 Endo的滴度为 4 .3× 1 0 1 2 空斑形成单位 (pfu) /L ,多聚酶链反应 (PCR)显示在转染的细胞中有人EndostatincDNA的存在 ,Westernblot显示转染细胞上清中有人Endostatin蛋白表达 ,台盼蓝染色显示其上清作用 72h后HUVEC的生长被抑制了 67% (P <0 .0 5)。结论　所制备的重组人Endostatin腺病毒载体能有效表达具有生物学活性的人Endostatin ,为进一步的研究奠定了基础