Omics Analysis of Blood-Responsive Regulon in Bordetella pertussis Identifies a Novel Essential T3SS Substrate

Bacterial pathogens sense specific cues associated with different host niches and integrate these signals to appropriately adjust the global gene expression. Bordetella pertussis is a Gram-negative, strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Though B. pertussis does not cause invasive infections, previous results indicated that this reemerging pathogen responds to blood exposure. Here, omics RNA-seq and LC–MS/MS techniques were applied to determine the blood-responsive regulon of B. pertussis. These analyses revealed that direct contact with blood rewired global gene expression profiles in B. pertussis as the expression of almost 20% of all genes was significantly modulated. However, upon loss of contact with blood, the majority of blood-specific effects vanished, with the exception of several genes encoding the T3SS-secreted substrates. For the first time, the T3SS regulator BtrA was identified in culture supernatants of B. pertussis. Furthermore, proteomic analysis identified BP2259 protein as a novel secreted T3SS substrate, which is required for T3SS functionality. Collectively, presented data indicate that contact with blood represents an important cue for B. pertussis cells.     

Sparse field level set method for non-convex Hamiltonians in 3D plasma etching profile simulations

Level set method [S. Osher, J. Sethian, J. Comput. Phys. 79 (1988) 12] is a highly robust and accurate computational technique for tracking moving interfaces in various application domains. It originates from the idea to view the moving front as a particular level set of a higher dimensional function, so the topological merging and breaking, sharp gradients and cusps can form naturally, and the effects of curvature can be easily incorporated. The resulting equations, describing interface surface evolution, are of Hamilton-Jacobi type and they are solved using techniques developed for hyperbolic equations. In this paper we describe an extension of the sparse field method for solving level set equations in the case of non-convex Hamiltonians, which are common in the simulations of the profile surface evolution during plasma etching and deposition processes. Sparse field method itself, developed by Whitaker [R. Whitaker, Internat. J. Comput. Vision 29 (3) (1998) 203] and broadly used in image processing community, is an alternative to the usual combination of narrow band and fast marching procedures for the computationally effective solving of level set equations. The developed procedure is applied to the simulations of 3D feature profile surface evolution during plasma etching process, that include the effects of ion enhanced chemical etching and physical sputtering, which are the primary causes of the Hamiltonian non-convexity.     

Simulation model of general human and humanoid motion

In the last decade we have witnessed a rapid growth of Humanoid Robotics, which has already constituted an autonomous research field. Humanoid robots (or simply humanoids) are expected in all situations of humans’ everyday life, “living” and cooperating with us. They will work in services, in homes, and hospitals, and they are even expected to get involved in sports. Hence, they will have to be capable of doing diverse kinds of tasks. This forces the researchers to develop an appropriate mathematical model to support simulation, design, and control of these systems. Another important fact is that today’s, and especially tomorrow’s, humanoid robots will be more and more humanlike in their shape and behavior. A dynamic model developed for an advanced humanoid robot may become a very useful tool for the dynamic analysis of human motion in different tasks (walking, running and jumping, manipulation, various sports, etc.). So, we derive a general model and talk about a human-and-humanoid simulation system. The basic idea is to start from a human/humanoid considered as a free spatial system (“flier”). Particular problems (walking, jumping, etc.) are then considered as different contact tasks – interaction between the flier and various objects (being either single bodies or separate dynamic systems).     

Modeling time criticality of information

In this paper, we continue the research on formal treatment of attributes of information, based on the computational approach. In this scenario, the usefulness of advisory information is measured by the decrease in complexity of a problem we need to solve. We propose to model the time criticality via usefulness of a piece of information which is received during the computation. As a modeling tool, we use deterministic finite automata.     

Synthesis of new hyperbranched urethane-acrylates and their evaluation in UV-curable coatings

Three series of hyperbranched urethane-acrylates (HB-UA), based on aliphatic hyperbranched polyesters and polyethyleneglycol acrylate, were prepared and evaluated for use in UV-curable coatings. UV-curing kinetics were monitored by FT-IR spectroscopy. The thermal and mechanical properties of UV-cured HB-UA were investigated by means of differential scanning calorimetry (DSC) and dynamic mechanical analysis (DMA) and correlated to the mechanical properties of coatings (hardness and flexibility). These new HB-UA's are very reactive and did not show oxygen inhibition. Obtained coatings have good mechanical properties and solvent resistance.     

The determination of molecular weights of polymers from critical concentrations of ternary systems polymer-polymer-solvent

The dependence of the critical concentration of the ternary system polymer-polymer-solvent on the molecular weight can be expressed by the relation where c_c is the critical concentration, M? the appropriate average of the molecular weight of both polymers; A and c_c∞ are constants for the given system. The possibility of determining the molecular weight of one of the polymers from the value c_c and the molecular weight of the known polymer with the aid of equation (1) is discussed.     

基于语音交互的脑瘫康复训练系统的设计

研究了使用人机交互的方式对脑瘫患儿进行康复训练的技术,设计了基于语音交互的脑瘫康复训练系统。该系统采用51单片机驱动整个系统,芯片采用LD3320语音芯片,下位机程序采用C语言编程,上位机采用Lab VIEW编程,通过串口传送的方式,把下位机接收到的命令传送到上位机,患儿通过识别上位机界面的图片,对应说出所要识别的文字,语音芯片进行识别,评价系统自动评判出患儿说的正确与否,并记录患儿每次识别所用的时间。案例实验验证了本设计的有效性。     

Improving practical exact string matching

We present improved variations of the BNDM algorithm for exact string matching. At each alignment our bit-parallel algorithms process a q-gram before testing the state variable. In addition we apply reading a 2-gram in one instruction. Our point of view is practical efficiency of algorithms. Our experiments show that the new variations are faster than earlier algorithms in many cases.     

Multi-view Superpixel Stereo in Urban Environments

Urban environments possess many regularities which can be efficiently exploited for 3D dense reconstruction from multiple widely separated views. We present an approach utilizing properties of piecewise planarity and restricted number of plane orientations to suppress reconstruction and matching ambiguities causing failures of standard dense stereo methods. We formulate the problem of the 3D reconstruction in MRF framework built on an image pre-segmented into superpixels. Using this representation, we propose novel photometric and superpixel boundary consistency terms explicitly derived from superpixels and show that they overcome many difficulties of standard pixel-based formulations and handle favorably problematic scenarios containing many repetitive structures and no or low textured regions. We demonstrate our approach on several wide-baseline scenes demonstrating superior performance compared to previously proposed methods.