EFFECT OF NITRATE AND PHOSPHATE ON ACCUMULATION OF β-CAROTENE ISOMERS IN DUNALIELLA SALINA

DunaIiella salina, a halotolerent unicellular green alga, can accurmulate a Iarge amount of β-caroteneunder environmental conditions. The isorners of β-carotene extIacted from D. salina culturedin medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-Talumins column. At least six isomers were found in different proportions depending on the culture me-dia's nitrate and/or phosphate concentrations. Nitrate and/or phosphae defidency was conducive tothe accumulation of totaI cis isomers but not of al1 trans isomer. lt is sUggeSted that 1 mmol/L KNO_3and 0.1 mmol/L KH_2, PO_4 are favourable for accumulation of total cis β-carotene.     

嵩县螃蟹沟隐爆角砾岩体与钼金矿化成因探讨

螃蟹沟隐爆角砾岩体是河南省嵩县西北部众多含钼、金角砾岩体之一,这些角砾岩体具有成群、成带分布的特点.螃蟹沟角砾岩体的定位与分布受北西向背形、北西向和北东向断裂构造的复合控制,由燕山期钙碱性岩体与岩脉的侵入,以及随后发生的隐蔽爆发-坍塌作用而生成了角砾岩体.其中包括了震碎角砾岩、隐爆角砾岩、侵入角砾岩及坍塌角砾岩.岩体被后期断裂构造作用所复合叠加,致使钼、金富集成矿,形成"多型一体"的钼金矿床.     

紫外线照射剂量对九孔鲍胚胎及幼虫的影响

采用不同的紫外线照射剂量的海水诱导九孔鲍产卵,观察其对卵子的受精率、畸形率和孵化率的影响.紫外线照射剂量的配制方法是:用紫外线照射过的最大剂量的海水与新鲜海水混合配制出各种剂量的海水.实验把雌亲鲍分成7组,每组20只,分别用紫外线照射剂量为0(对照组)、450、600、750、900、1 050和1 200mW.h/dm3的海水进行刺激产卵.试验重复3次.结果表明,紫外线照射剂量在1 200mW.h/dm3以内,对卵子的受精率影响不大;紫外线照射剂量在750mW.h/dm3以内,对卵子的畸形率影响不大,但在900~1 200mW.h/dm3,对卵子的畸形率影响很大;紫外线照射剂量在600mW.h/dm3以内,对卵子的孵化率没有什么影响,但在750~1 200mW.h/dm3,对卵子的孵化率影响很大.综合考虑卵子的受精率、畸形率和孵化率,九孔鲍催产的适宜的紫外线照射剂量应为450~750mW.h/dm3.     

大黄鱼(Pseudosciaena crocea)转铁蛋白(Tf)基因克隆及序列分析

提要应用RNeasy animal mini试剂盒抽提大黄鱼total RNA,逆转录合成模板cDNA;同时综合分析从GenBank数据库查询到已报道的转铁蛋白(Tf)基因序列,先设计并合成扩增引物扩增出大黄鱼转铁蛋白部分基因序列,再应用RACE技术,克隆出大黄鱼转铁蛋白全长cDNA基因,全长2461个核苷酸,编码661个氨基酸。应用WCG软件包,采用DNADIST和NEIGHBOR分析方法,构建了转铁蛋白系统进化树。树图显示与传统的分类地位大致相符,可以看出转铁蛋白在海水鱼和淡水鱼的进化上的差异,同时也显示大黄鱼Tf与真鲷的同源性最高。     

南海南部晚中新世的放射虫及其环境探讨

南海是东亚古季风产物的主要沉积盆地，保存着比陆地更加完整和连续的沉积记录。详细分析了ODP1143号钻井晚中新世的放射虫化石群，主要根据标志种Diartus petterssoni,D.hughesi和Stichocorys delmontensis等的分布特征，分别建立了南海南部晚中新世的RN6，RN7和RN8等3个放射虫化石带，并讨论了其地层年龄。探讨了以地层中放射虫的丰度变化特征等在南海南部作为东亚古夏季风活动替代性指标的可能性，初步说明东亚古夏季风可能早于8．7Ma B.P．出现，约在8．24Ma B.P.强化达到高峰，认为与印度季风的出现几乎同步或略早。     

Seasonal variation of global surface pressure and water vapor

Previous studies have shown that the seasonal variation of global-mean surface pressure ( p _s) results from variation of global-mean water vapor pressure ( p _w). The current study, employing the global data generated by Version 1 of the Goddard Earth Observing System (GEOS-1) Data Assimilation System, shows that seasonal variations of regional p _s and p _w tend to be out of phase (particularly in the subtropics of the two hemispheres) and that the magnitude of the former variation is generally much larger than that of the latter. The seasonal variations of these two quantities are maintained by airmass and water vapor transports by the global divergent circulation, which is driven by the latent heat released by cumulus convection over the water vapor sink, as the "water mass forcing" mechanism predicted. Since p _w and p _s are used often in depicting the climate system, assessments of climate change in terms of the global-mean and regional variations of these two variables should be interpreted with caution.     

RADIOLARIA IN CORES FROM THE OKINAWA TROUGH

A total of 149 genus and 238 species of Radiolaria in cores from four stations in the Okinawa Trough are examined in this paper. Water depths and cores lengths at these stations are 602-1900 m and 1.7-2.4 m respectively (see Fig. 1). Based on the distributional characteristic of Radiolaria and the radio-larian temperature number (Tr), the climatic fluctuations since the last 150 000 years and the stratigra-phic classification are also discussed.