用Hela229细胞培养法研究呼吸道肺炎衣原体感染

本文用Ｈｅｌａ２２９细胞培养与单克隆抗体免疫荧光法对９４０例咽拭子及２２４例纤支镜取材标本进行肺炎衣原体分离鉴定。结果正常组、上呼吸道感染组、下呼吸道感染组、肺部肿瘤组的咽拭子标本分离率分别为０％（０／２４８）,２．１３％（１０／４６８）,２．１％（３／１４６）,１．２８％（１／７８）。上呼吸道感染组和下呼吸道感染组的分离率均高于正常组和肺部肿瘤组。前二组与正常组的差异有显著性意义（Ｐ＜０．０５）,与肿瘤组的差异无显著性意义（Ｐ＞０．０５）。下呼吸道感染组、肺部肿瘤组的纤支镜取材分离率分别为１０．９６％（     

NSA－Ⅱ型生理信号处理系统

介绍一种ＩＢＭ－ＰＣ系列微机生理信号采集、处理系统。该系统信号采集卡集程控放大、脉冲甄别、Ａ／Ｄ、Ｄ／Ａ等电路于一体,可满足各种生理信号的采集需要。采集信号时可长时间显示,动态观察。采样后,按需要给出各种图表与结果。部分处理方法采用独特数学模型,使结果更加精确。     

单增李斯特菌末端氧化酶亚基QoxB的生物学功能探究

【目的】本研究旨在构建单增李斯特菌末端细胞色素aa3氧化酶亚基qoxB基因缺失株,并探索其在细菌生长及感染过程中发挥的生物学功能。【方法】利用同源重组方法构建获得缺失株ΔqoxB后,对野生株EGD-e和缺失株ΔqoxB的生长能力、细菌运动能力和细胞内黏附、侵袭、增殖及胞内迁移能力进行比较,同时利用荧光定量PCR方法检测ΔqoxB中鞭毛相关基因转录水平的变化。【结果】缺失qoxB基因后细菌在体外培养过程中生长能力没有差异,细菌的鞭毛运动能力显著降低,在30℃培养24 h和48 h后ΔqoxB运动圈直径分别较EGD-e下降35.86%和34.20%,且22个鞭毛相关基因转录水平显著降低。通过细胞感染试验发现缺失qoxB基因后细胞黏附、侵袭、增殖及胞内迁移能力均显著下降。【结论】本研究首次证实末端氧化酶亚基QoxB能降低单增李斯特菌的运动能力和对细胞的感染能力,此研究为进一步阐明末端细胞色素氧化酶影响单增李斯特菌的致病机制提供重要依据。     

Synthesis of (±)-16-thioketal and 16-keto prostaglandins

The synthesis of ((±)-16-thioketal and 16-keto PGE₂ methyl ester ( and ) is herein described.     

SPLUNC1 Regulates Cell Progression and Apoptosis through the miR-141-PTEN/p27 Pathway,but Is Hindered by LMP1

Little is known about the role of the host defensive protein short palate, lung and nasal epithelium clone 1 (SPLUNC1) in the carcinogenesis of nasopharyngeal carcinoma (NPC). Here we report that SPLUNC1 plays a role at a very early stage of NPC carcinogenesis. SPLUNC1 regulates NPC cell proliferation, differentiation and apoptosis through miR-141, which in turn regulates PTEN and p27 expression. This signaling axis is negatively regulated by the EBV-coded gene LMP1. Therefore we propose that SPLUNC1 suppresses NPC tumor formation and its inhibition by LMP1 provides a route for NPC tumorigenesis.     

Vascular Endothelial Growth Factor +936C/T, –634G/C, –2578C/A,and –1154G/A Polymorphisms with Risk of Preeclampsia: A Meta-Analysis

Background

Emerging evidence showed that VEGF gene polymorphisms are involved in the regulation of VEGF protein expression, thus increasing an individual''s susceptibility to preeclampsia (PE); but individually published results are inconclusive. The aim of this meta-analysis was to investigate the associations between VEGF gene polymorphisms and PE risk.

Methods

A systematic literature search of MEDLINE, Embase, Web of Science, and CNKI (Chinese National Knowledge Infrastructure) databases was conducted. Statistical analyses were performed using STATA 12.0 software and Review manager 5.1. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of associations.

Results

According to the inclusion criteria, 11 case-control studies were finally included in this meta-analysis. A total of 1,069 PE cases and 1,315 controls were included in this study. Our meta-analysis indicated that VEGF +936C/T (T vs. C, OR = 1.52, 95%CI = 1.08–2.12) or −634G/C polymorphism (C vs. G, OR = 1.24, 95% CI = 1.03–1.50) was associated with the risk of PE, whereas there was no association between −2578C/A (A vs. C, OR = 0.98, 95%CI = 0.82–1.16) or −1154G/A (A vs. G, OR = 1.30, 95%CI = 0.94–1.78) polymorphism and PE risk in our study.

Conclusion

Our meta-analysis suggested that VEGF −2578C/A or −1154G/A polymorphism had no association with PE risk in all examined patients, whereas there was an association between VEGF +936C/T or −634G/C polymorphism and risk of PE.     

灰树花多糖对乳腺癌细胞MCF-7的凋亡作用

采用MTT法测定不同给药浓度的灰树花多糖(PGF) (1、10、20、50、100和200 μg/mL)在24、48和72 h对乳腺癌细胞(MCF-7)增殖的抑制率,并采用Hoechst染色与流式细胞技术观察20、50和100 μg/mL PGF给药24 h后MCF-7的凋亡情况,同时采用Western blotting对20、50、100 μg/mL PGF给药24 h后MCF-7细胞中Bax、Bcl-2、Pro-Caspase-3以及Cleaved Caspase-3的蛋白表达水平进行检测。研究发现PGF给药24、48和72 h后对MCF-7的增殖均有显著的抑制作用。随着PGF给药浓度增加,MCF-7细胞核裂解增多,细胞凋亡数量增多。PGF 20、50和100 μg/mL给药对MCF-7细胞Bax、Bcl-2、Pro-Caspase-3以及Cleaved Caspase-3的蛋白表达水平可见显著性差异。     

不同佐剂对含S+PreS1融合抗原的乙型肝炎病毒颗粒疫苗免疫原性的影响

本研究在前期工作基础上,用CHO细胞表达的含PreS1+S融合抗原的新型基因工程HBV颗粒疫苗(HBSS1)与Al(OH)3、CpG及CpG+Al(OH)3等佐剂配伍,在Balb/C小鼠模型上研究不同佐剂对HBV颗粒疫苗肌肉注射后免疫应答的影响,主要包括抗体滴度、抗体亚型分类及特异性细胞免疫(γ-IFNELISpot检测)。结果表明:CpG佐剂结合HBSS1颗粒疫苗可快速诱导(单针免疫)高水平的抗PreS1及S抗体,IgG2a/IgG1比率1,同时可诱导较高抗原特异的细胞免疫应答;Al(OH)3+CpG双佐剂组一次免疫后可诱导产生最高的抗S抗体滴度(1:105),其产生的抗体亚类包括IgG1、IgG2a与IgG2b;在S抗原N端(13~49aa)存在优势CTL表位。结论:CpG佐剂结合HBSS1颗粒疫苗应是发展新型治疗性乙肝疫苗的较佳选项。     

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Whole-mount immunohistochemical analysis for imaging the entire vasculature is pivotal for understanding the cellular mechanisms of branching morphogenesis. We have developed the limb skin vasculature model to study vascular development in which a pre-existing primitive capillary plexus is reorganized into a hierarchically branched vascular network. Whole-mount confocal microscopy with multiple labelling allows for robust imaging of intact blood vessels as well as their cellular components including endothelial cells, pericytes and smooth muscle cells, using specific fluorescent markers. Advances in this limb skin vasculature model with genetic studies have improved understanding molecular mechanisms of vascular development and patterning. The limb skin vasculature model has been used to study how peripheral nerves provide a spatial template for the differentiation and patterning of arteries. This video article describes a simple and robust protocol to stain intact blood vessels with vascular specific antibodies and fluorescent secondary antibodies, which is applicable for vascularized embryonic organs where we are able to follow the process of vascular development.Download video file.^{(59M, mov)}