A new method of preparing fiber-optic DNA biosensor and its array for gene detection

A new method of preparing fiber-optic DNA biosensor and its array for the simultaneous detection of multiple genes is described. The optical fibers were first treated with poly-l-lysine, and then were made into fiber-optic DNA biosensors by adsorbing and immobilizing the oligonucleotide probe on its end. By assembling the fiber-optic DNA biosensors in a bundle in which each fiber carried a different DNA probe, the fiber-optic DNA biosensor array was well prepared. Hybridization of fluorescent- labeled cDNA of p53 gene, N-ras gene and Rb1 gene to the DNA array was monitored by CCD camera. A good result was achieved.     

河北小五台山夏季鸟类初步调查

小五台山的鸟类,尚未见有系统报道。本省于1981年对该地组织了综合考察。现将我们于1981、1982两年的6—10月份所得鸟类调查资料整理如下。自然环境概况小五台山位于河北省西北部,东经114°50′—115°15′,北纬39°40′—40°10′。该山属恒山余脉,山势多较陡峻,五个台(峰)的绝对标高分别为2671—2882米,是河北省最高的山峰。该山目前所见均为次生植被,但随海拔高     

用于基因探测的光纤DNA传感器及其阵列的一种新的制备方法

报道了一种光纤DNA传感器及其阵列的新的制备方法,以及应用该阵列同时进行多基因检测的结果.光纤经0.2%的poly-l-lysine处理后,可用于吸附寡核苷酸探针,探针经固定后即制成一种光纤DNA传感器.将携带不同基因探针的传感器排成一个阵列,可用于多个基因的同时检测.用含有p53,N-ras,Rb1基因探针的阵列进行实验的结果表明,这种传感器及其阵列可用于特定基因的探测,或多个基因的同时检测.