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裸粒水稻同源四倍体的人工诱导 总被引:2,自引:0,他引:2
裸粒水稻成熟胚在含不同浓度2,4-D与激动素的培养基上愈伤组织的诱导率不同,2,4-D为2.0mg/L、激动素为1.0mg/L时愈伤组织的诱导率最高,达53.3%。分别以200mg/kg、500mg/kg、800mg/kg和1000mg/kg浓度的秋水仙碱处理二倍体愈伤组织,结果都获得了裸粒水稻的同源四倍体无性系,但以500~1000mg/kg的浓度处理效果较好,800mg/kg的浓度处理最为理想,加倍效率达30.0%。
Abstract:The induced frequencies of wound calluses in naked seed rice were different on the culture media of different concentrations of 2,4-D and KT. It was the highest to 53.3%when 2,4-D was 2.0mg/L and KT was 1.0mg/L. The diploid wound calluses were respectively treated by colchicine of 200mg/kg, 500mg/kg, 800mg/kg and 1000mg/kg. The clonal lines of autotetraploid in naked seed rice were obtained in all treatments. The induced effects were better in 500~1000mg/kg, 800mg/kg was the best and its doubling efficiency was up to 30.0%. 相似文献
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激发学生对遗传学实验学习兴趣的教学方法探索 总被引:1,自引:0,他引:1
通过增设研究性较强或由学生自主设计的实验项目, 采用生动有趣的案例式教学和问题式教学, 用“多元性”的教学理念设计实验教学环节, 结合综合性的教学手段, 有效激发了学生对遗传学实验的学习兴趣。 相似文献
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