核素显像对恶性肿瘤骨转移的临床价值

本文对150例患者进行了核素全身骨显像，诊断转移性骨肿瘤123例，并与X线片结果相对比，其灵敏度高于X线检查。骨转移发生率为82％。150例检查者，其中不明肿瘤发生部位，经骨显像后确诊找到原发灶者6例。故全身骨显像对转移性骨肿瘤有很高的灵敏度，并能较早地发现骨转移灶，对寻找原发灶有所帮助。     

99锝m N-2-巯基吡啶-N-氧化物与99锝m-甲氧基异丁基异腈对犬急性心肌梗死模型的对照研究

Objective The purpose of the present study is to compare the pharmacokinetic and biodistribution properties of 99Tcm N-mercaptopyridine-N-oxide (99 Tcm N-MPO) with 99 Tcm-sestamibi (99 Tcm-MIBI) in normal dogs, and to investigate the potential of 99TcmN-MPO as a myocardial perfusion agent in canines with acute myocardial infarction. Methods Twelve healthy mongrel dogs were injected intravenously with 99TcmN-MPO (n = 6) or 99Tcm-MIBI (n = 6). Tracer kinetics in body fluids were determined by collecting blood of 1 ml via a femoral vein catheter at 30 s, 1,2,3,4,5, 10, 20, 30, 40, 60and 90 min post-injection (p. i.). The collected blood samples were weighed and counted for radioactivity in a γ-counter. Anterior and posterior planar γ-camera images were collected at 10, 20, 30, 60, 90, and 120 min after injection, with organ uptake quantified by region-of-interest (ROIs) analysis. For comparison, 99Tcm-MIBI was also evaluated in the same twelve dogs. Canine infarct models were set up by micro-invasive interventional embolization. SPECT images in the canine infarct model were collected 24 hours after myocardial infarction at 30 min and 60 min after the administration of 99Tcm N-MPO (n = 5) or 99Tcm-MIBI (n = 5). Results Both of 99Tcm N-MPO and 99Tcm-M1BI had a rapid blood clearance with less than 50% of initial radioactivity remaining at 1 min [99TcmN-MPO: (35. 77 ± 6. 31)% ID/mg ,99Tcm-MIBI (34. 46 ± 6. 83) % ID/mg] and less than 5% at 30 min p. i. [99Tcm N-MPO(3. 11 ± 1.44) % ID/mg,99Tcm-MIBI (2.93 ±0. 39)% ID/mg] . After injection, 99TcmN-MPO showed significant accumulation in the myocardium and prolonged retention. This rapid liver clearance of 99TcmN-MPO led to favorable heart-to-liver ratios, reaching values of 0. 54 ±0. 06 at 10 min, 1.02 ±0. 06 at 30 min, and 1.38 ±0. 06 at 60 min p. i.In contrast, the heart/liver ratio of 99Tcm-MIBI remained low at all time points (0. 46 ± 0. 03 at 10 min,0. 63 ±0. 03 at 30 min, and 0. 62 ± 0. 12 at 60 min p. i.). SPECT imaging studies in canines with acute myocardial infarction indicated that good visualization of the left ventricular wall and perfusion defects could be achieved at 30 min after administration of 99TcmN-MPO, but not 99Tcm-MIBI. Conclusion The combination of high heart uptake and rapid liver clearance makes 99TcmN-MPO a promising new radiotracer for myocardial perfusion imaging.     

核素心血池显像测定76例冠心病患者左室功能的分析

核素心血池显像测定７６例冠心病患者左室功能的分析孙月桂，金钟男，王琳，迟鸿滨冠心病合并左室舒张功能障碍日益受到重视，我们报道７６例老年前期及老年冠心病患者左室功能的测定结果。资料和方法冠心病组７６例，男６２例，女１４例，年龄５５～７６岁，平均６４．４...     

~(99)mTC─—MAA核素显像诊断下肢深静脉血栓形成及肺栓塞临床评价

为了研究下肢深静脉血栓形成DVT与肺栓塞PE发病之间的关系，我科于1991－03～1997－03用99mTC─—MAA（大颗粒聚合白蛋白）进行下肢深静脉核素显像RNV，并随后行核素肺血流灌注显像PPS，现将阳性34例患者报告如下。1资料与方法1.1临床资料。39例中，男性32例，女性7例。年龄自35～70岁，平均年龄49．3岁。病程1个月～20年。1.2显像剂。用标记药MAA（上海红旗药厂生产的药盒。）1.3仪器与方法。ElcintApex─—SP4SPECT（单光子发射型电算机断层仪）。常规检查方法：患者取仰卧位。先于双下肢足背静脉滴注0．9％氯化钠注射液。在…     

99锝m N-2-巯基吡啶-N-氧化物与99锝m-甲氧基异丁基异腈对犬急性心肌梗死模型的对照研究

目的 在正常和心肌梗死犬模型上,研究99TcmN-2-巯基吡啶-N-氧化物(99TcmN-MPO)的药物代谢动力学特征、生物学分布特征和对急性心肌梗死的诊断能力,并与传统示踪剂99锝m-甲氧基异丁基异腈(99Tcm-MIBI)进行对比研究.方法 正常杂种犬12只.静脉注射99TcmN-MPO,于不同时间点(30 s及1、2、3、4、5、10、20、30、40、60和90 min)静脉分别采血1 ml,经γ探测器测量其放射活性;注药后10、20、30、60、90及120 min行全身SPECT显像,并于不同器官上各取同样大小的ROI,测量其放射性计数进行定量研究.每只犬均注射相同剂量的99TcmMIBI,进行相同的实验作为对照.介入学方法构建犬急性心肌梗死模型,24 h后,静脉注入99TcmN-MPO(5只)和99Tcm-MIBI(5只),并于注药后30、60min进行心肌SPECT显像.结果 在静脉注射90 min内,99TcmN-MPO和99Tcm-MIBI均表现为快速的血液清除.两种示踪剂的初始注射剂量均为370 MBq.注射后1 min,每毫克血浆的放射活性小于初始注射剂量的50%[99TcmN-MPO为(35.77±6.31)%ID/mg;99Tcm-MIBI为(34.46±6.83)%ID/mg],30 min时＜5%[99TcmN-MPO:(3.11±1.44)%ID/mg;99Tcm-MIBI:(2.93±0.39)%ID/mg].99TcmN-MPO在心脏的浓聚明显,且存留时间很长,由于其在肝脏清除的速度较快,因此可获得良好的心/肝摄取比值,注射后10 min为0.54±0.06,30 min为1.02±0.06,60 min上升到1.38±0.06.相比之下,99Tcm-MIBI的心/肝摄取比值上升较为缓慢(注射后10 min为0.46±0.03,30 min为0.63±0.03,60 min为0.62±0.12).犬心肌梗死后SPECT心肌断层扫描显示,在注射99TcmN-MPO 30 min后,心脏与肝脏分界清楚,可清楚显示心肌缺血、梗死灌注缺损区域、范围和程度;而99Tcm-MIBI注射后60 min,心脏和肝脏的分界依然不清,尤其是肝脏的高放射性对心脏下壁和左心室壁的影响很大,对心脏左心室壁的心肌梗死灌注缺损区域的显示不佳.结论 99TcmN-MPO具有心肌摄取量较高,肝脏代谢快的特点,是一种具有广阔临床应用前景的SPECT心肌灌注成像显像剂.

Abstract：

Objective The purpose of the present study is to compare the pharmacokinetic and biodistribution properties of 99Tcm N-mercaptopyridine-N-oxide (99 Tcm N-MPO) with 99 Tcm-sestamibi (99 Tcm-MIBI) in normal dogs, and to investigate the potential of 99TcmN-MPO as a myocardial perfusion agent in canines with acute myocardial infarction. Methods Twelve healthy mongrel dogs were injected intravenously with 99TcmN-MPO (n = 6) or 99Tcm-MIBI (n = 6). Tracer kinetics in body fluids were determined by collecting blood of 1 ml via a femoral vein catheter at 30 s, 1,2,3,4,5, 10, 20, 30, 40, 60and 90 min post-injection (p. i.). The collected blood samples were weighed and counted for radioactivity in a γ-counter. Anterior and posterior planar γ-camera images were collected at 10, 20, 30, 60, 90, and 120 min after injection, with organ uptake quantified by region-of-interest (ROIs) analysis. For comparison, 99Tcm-MIBI was also evaluated in the same twelve dogs. Canine infarct models were set up by micro-invasive interventional embolization. SPECT images in the canine infarct model were collected 24 hours after myocardial infarction at 30 min and 60 min after the administration of 99Tcm N-MPO (n = 5) or 99Tcm-MIBI (n = 5). Results Both of 99Tcm N-MPO and 99Tcm-M1BI had a rapid blood clearance with less than 50% of initial radioactivity remaining at 1 min [99TcmN-MPO: (35. 77 ± 6. 31)% ID/mg ,99Tcm-MIBI (34. 46 ± 6. 83) % ID/mg] and less than 5% at 30 min p. i. [99Tcm N-MPO(3. 11 ± 1.44) % ID/mg,99Tcm-MIBI (2.93 ±0. 39)% ID/mg] . After injection, 99TcmN-MPO showed significant accumulation in the myocardium and prolonged retention. This rapid liver clearance of 99TcmN-MPO led to favorable heart-to-liver ratios, reaching values of 0. 54 ±0. 06 at 10 min, 1.02 ±0. 06 at 30 min, and 1.38 ±0. 06 at 60 min p. i.In contrast, the heart/liver ratio of 99Tcm-MIBI remained low at all time points (0. 46 ± 0. 03 at 10 min,0. 63 ±0. 03 at 30 min, and 0. 62 ± 0. 12 at 60 min p. i.). SPECT imaging studies in canines with acute myocardial infarction indicated that good visualization of the left ventricular wall and perfusion defects could be achieved at 30 min after administration of 99TcmN-MPO, but not 99Tcm-MIBI. Conclusion The combination of high heart uptake and rapid liver clearance makes 99TcmN-MPO a promising new radiotracer for myocardial perfusion imaging.     

HSV1-tk报告基因真核表达载体的构建及其在人肺腺癌AGZY细胞中的表达

目的：构建含有HSV1-tk（ Herpes simplex virus type 1 thymidine kinase, HSV1-tk）基因的真核表达载体,并检测其在人肺腺癌AGZY细胞系中的表达。方法应用PCR反应从质粒pHSV106质粒中扩增HSV1-tk基因后与pMD18-T载体连接,构建重组质粒pHSV1-tk/18T。将测序正确的重组质粒插入plRES2-EGFP 载体内,通过LipofectamineTM 2000将表达载体转染人肺腺癌AGZY细胞。结果酶切鉴定结果表明扩增的HSV1-tk基因序列正确;用荧光显微镜观察HSV1-tk基因的转入和表达;RT-PCR和Western blot结果显示在AGZY细胞中HSV1-tk基因在转录水平和蛋白水平均可以正确表达。MTT结果显示转染后AGZY细胞与未转染细胞在细胞增殖能力方面无明显差别。结论成功构建HSV1-tk报告基因的真核表达载体,在人肺腺癌AGZY细胞中能有效表达。     

SPECT骨盆平面像与断层像早期诊断非创伤性的股骨头缺血坏死的对比分析

放射性核素骨盆显像可帮助早期诊断股骨头缺血坏死‘”，而且也有报道认为在股骨头平面显像不明显或可疑时，做SPECT断层显像，可提高股骨头缺血坏死的检出率‘”。我们通过268例股骨头平面像与断层像对比分析，探讨其方法早期诊断股骨头缺血坏死的临床意义。1资料和方法1．1一般资料。本级共268例，女40例，男228例。平均年龄42岁（24～65）。其中正常显像为129例，异常显像为139例，包括单侧股骨头缺血坏死95例，双侧股骨头缺血坏死44例，共183个股骨头受累。1．2材料。MDP（亚锡亚甲基二磷酸盐）由无锡原子医学研究所提供，SPECT为…     

卵巢巧克力囊肿无水乙醇硬化治疗前后卵巢功能的变化

目的探讨超声引导下无水乙醇硬化治疗卵巢巧克力囊肿前后卵巢功能的变化。方法对45例卵巢巧克力囊肿患者在超声引导下进行介入治疗,分别于硬化治疗前及治疗后3、6、12个月检测患侧卵巢血流显示状况,并测量卵巢动脉收缩期峰值流速（Vs）、舒张期流速（Vd）及阻力指数（RI）,同时进行血清性激素6项测定,即垂体泌乳素（PRL）、卵泡生成素（FSH）、促黄体生成素（LH）、雌二醇（E2）、孕酮（P）和睾酮（T）。结果无水乙醇硬化治疗后3、6及12个月,患侧卵巢血液供应状况较治疗前有明显改善,患侧卵巢动脉Vs、Vd增高,RI减低,与治疗前相比有显著性差异（P〈0.05）。20例单侧卵巢巧克力囊肿（对侧卵巢正常）的患者各项性激素水平治疗后3个月与治疗前相比无显著性差异（P〉0.05）;15例单侧卵巢巧克力囊肿（对侧卵巢切除）患者的血清PRL、E2、P、T水平和10例双侧卵巢巧克力囊肿患者的PRL、E2、P水平治疗后3个月检查结果较治疗前明显降低,治疗后3个月与治疗前相比有显著性差异（P〈0.05）;治疗后6、12个月各组性激素水平基本恢复正常范围。结论无水乙醇硬化治疗卵巢巧克力囊肿能够改善患者卵巢的血液供应,并调整卵巢性激素水平维持在正常范围内。     

99锝m N-2-巯基吡啶-N-氧化物与99锝m-甲氧基异丁基异腈对犬急性心肌梗死模型的对照研究

Objective The purpose of the present study is to compare the pharmacokinetic and biodistribution properties of 99Tcm N-mercaptopyridine-N-oxide (99 Tcm N-MPO) with 99 Tcm-sestamibi (99 Tcm-MIBI) in normal dogs, and to investigate the potential of 99TcmN-MPO as a myocardial perfusion agent in canines with acute myocardial infarction. Methods Twelve healthy mongrel dogs were injected intravenously with 99TcmN-MPO (n = 6) or 99Tcm-MIBI (n = 6). Tracer kinetics in body fluids were determined by collecting blood of 1 ml via a femoral vein catheter at 30 s, 1,2,3,4,5, 10, 20, 30, 40, 60and 90 min post-injection (p. i.). The collected blood samples were weighed and counted for radioactivity in a γ-counter. Anterior and posterior planar γ-camera images were collected at 10, 20, 30, 60, 90, and 120 min after injection, with organ uptake quantified by region-of-interest (ROIs) analysis. For comparison, 99Tcm-MIBI was also evaluated in the same twelve dogs. Canine infarct models were set up by micro-invasive interventional embolization. SPECT images in the canine infarct model were collected 24 hours after myocardial infarction at 30 min and 60 min after the administration of 99Tcm N-MPO (n = 5) or 99Tcm-MIBI (n = 5). Results Both of 99Tcm N-MPO and 99Tcm-M1BI had a rapid blood clearance with less than 50% of initial radioactivity remaining at 1 min [99TcmN-MPO: (35. 77 ± 6. 31)% ID/mg ,99Tcm-MIBI (34. 46 ± 6. 83) % ID/mg] and less than 5% at 30 min p. i. [99Tcm N-MPO(3. 11 ± 1.44) % ID/mg,99Tcm-MIBI (2.93 ±0. 39)% ID/mg] . After injection, 99TcmN-MPO showed significant accumulation in the myocardium and prolonged retention. This rapid liver clearance of 99TcmN-MPO led to favorable heart-to-liver ratios, reaching values of 0. 54 ±0. 06 at 10 min, 1.02 ±0. 06 at 30 min, and 1.38 ±0. 06 at 60 min p. i.In contrast, the heart/liver ratio of 99Tcm-MIBI remained low at all time points (0. 46 ± 0. 03 at 10 min,0. 63 ±0. 03 at 30 min, and 0. 62 ± 0. 12 at 60 min p. i.). SPECT imaging studies in canines with acute myocardial infarction indicated that good visualization of the left ventricular wall and perfusion defects could be achieved at 30 min after administration of 99TcmN-MPO, but not 99Tcm-MIBI. Conclusion The combination of high heart uptake and rapid liver clearance makes 99TcmN-MPO a promising new radiotracer for myocardial perfusion imaging.     

SPECT/PET 的18FDG-PET 显像与MSCT融合对肺癌纵隔淋巴结转移的诊断价值

非小细胞肺癌(NSCLC)术前纵隔淋巴结分期是进行治疗选择的关键[1].文献报道正电子发射断层显像术显像(PET)对淋巴结转移分期的诊断价值明显高于CT[2].本研究对66例NSCLC患者进行18FDG-PET显像,旨在研究SPECT/PET的18FDG-PET显像与异机多层螺旋CT图像融合对的淋巴结转移分期的临床诊断价值.