抗衰老药“春回”对正常细胞和肿瘤细胞DNA合成的探索

根据中医古方配制的“春回”(胶囊),经一些医学单位初步试验,对延缓人体衰老、恢复青春活力具有一定的作用。这引起了人们很大的兴趣和关注。缘此,从细胞水平、分子水平研究“春回”的功效和作用机理很有必要。肿瘤是危害人体健康、使人短寿的多发病、常见病。为此,我们测定了“春回”水浸液对小白鼠正常骨髓细胞和小白鼠     

克感利咽口服液对流感病毒感染小鼠脾淋巴细胞释放氧自由基和NO水平的调节作用

流感病毒作为外源性抗原侵入机体 ,激活巨噬细胞和嗜中性白细胞产生氧自由基已被我们和其他学者的实验所证实〔1〕,而流感病毒感染对次级免疫器官脾脏Ｔ、Ｂ淋巴细胞产生活性氧和一氧化氮 (ＮＯ)自由基的体内实验尚未见报道。我们依据本项课题的研究目标和中药复方的作用特点 ,从中医整体观出发 ,研究了流感病毒ＦＭ1 呼吸道感染对小鼠脾淋巴细胞释放氧自由基和ＮＯ的影响及其克感利咽口服液对流感病毒感染引起脾淋巴细胞应激升高的调节作用。1　材料和方法1 1　动物与毒株　清洁级ＮＩＨ小鼠 ,13～ 15ｇ雌雄兼用 ,由解放军第三○一医院动物…     

克感利咽口服液对流感病毒感染诱导ROTS块形成和白细胞吞噬功能下降的调节作用--布氏显微镜在中药药理学中的应用实例

克感利咽口服液系中国中医研究院广安门医院的临床经验方研制而成的三类新药,由金银花、黄芩、荆芥、栀子、僵蚕、地黄等药物组成,具有疏风清热、解毒利咽功效.用于治疗急性上呼吸道感染(风热外侵、邪热内扰证候)疗效显著.药理实验证明,本药对流感病毒甲3、乙型和常见呼吸道病菌具有明显的抑制作用[1],对多种动物模型有明显的解热、抗炎、镇痛和调节免疫功能作用[2,3],为进一步了解本药对流感病毒诱导的氧化应激反应的调节作用,本文采用先进的布氏显微镜检查系统,对各组小鼠进行了氧自由基足迹(ROTS块血片)和白细胞吞噬功能(活血片)实验观察,得到非常有意义的结果,现将实验结果报告如下.     

ACUPUNCTURE TREATMENT OF SCIATICA AND A PRELIMINARY STUDY OF THE ANALGESIC MECHANISM

This article reports 145 cases of moderate andsevere sciatica treated with acupuncture.The totaleffective rate was 95.7%,the basically cured rate42.7%.The average number of acupuncture sessionsfor the basically cured was 15.The chief acupointsused were Shenshu (UB 23),Huatuojiaji L_4,L_5 (Extra21),Dachangshu (UB 25),Guanyuanshu (UB 26),and Weizhong (UB 40).Additional auxiliary pointsused for the Shaoyang type were Huantiao (GB 30),Yanglingquan (GB 34) and Huanzhong (GB 39).Forthe Taiyang type they were Zhibian (UB 54),Cheng-shu (UB 57) and Kunlun (UB 60).The points takenfor acute and severe pains were Sizhukong (SJ 23),Renzhong (Du 26) and Yanglao (SI 6).The casesof wind and cold type and kidney deficiency typewere given moxibustion and cupping in addition.The results of acupuncture treatment show theprocedure to be effective in relieving the pain of alltypes of sciatica,including cases of long duration.Follow-up of the cured patients indicates that thesecondary type is apt to relapse.Fifteen patients suffering from acute sciatica hadserum 5-HT increased after acupuncure.In ten ofthe 15 cases,plasma cAMP,cGMP and PGE_2 in-creased and PGF_(2α) decreased after acupuncture.These findings suggest that the physiological basis ofthe regulatory effect of acupuncture may be bioactivesubstances,a possibility meriting further research.     

Ge─132对烈酒精之氧化损伤的保护作用

目的：探讨β－羧乙基锗倍半氧化物（Ｇｅ－１３２）在体内清除自由基、抗氧化作用。方法：下列药物作用于烈酒造成氧应激态的大鼠，观察其肝线粒体超氧化物歧化酶活性和肝脏的病理变化，并与维生素Ｃ进行了比较。Ｗｉｓｔａｒ大鼠随机分为４组。正常对照（Ａ），烈酒（Ｂ），烈酒＋维生素Ｃ（Ｃ），烈酒＋Ｇｅ－１３２（Ｄ）。各组大鼠常规饲喂。每种药的给药方法如下：烈酒（含体积分数为５５％乙醇），５ｇ／（ｋｇｄ），从第８天开始ｉｇ共２周；维生素Ｃ２００ｍｇ／（ｋｇｄ），从第１天开始ｓｃ共３周；Ｇｅ－１３２２００ｍｇ／（ｋｇｄ），从第１天开始ｉｇ共３周。结果：肝线粒体铜锌超氧化物歧化酶活性，烈酒组与对照组比较，其活性下降（Ｐ＜０．０５），烈酒＋Ｇｅ－１３２和烈酒＋维生素Ｃ组均与烈酒组比较，其活性有上升趋势。肝脂肪变性在烈酒＋Ｇｅ－１３２组较烈酒组为轻，烈酒＋维生素Ｃ组病变不明显。结论：Ｇｅ－１３２在一定程度上保护肝线粒体铜锌超氧化物歧化酶活性，改善肝脂肪变性，但对肝脏病变的保护作用不如高浓度维生素Ｃ。     

β-羧乙基锗倍半氧化物和还原型谷胱甘肽及维生素B1配伍抗氧化作用

 目的：探讨β-羧乙基锗倍半氧化物(Ge-132)与还原型谷胱甘肽(GSH)和维生素B₁(VB₁)联合应用在体内抗氧化的作用。方法：观察这些药物对乙醇诱发大鼠氧应激态作用下的抗氧化酶活性变化。结果：肝线粒体总超氧化物歧化酶和铜锌超氧化物歧化酶活性，乙醇组与正常对照组比较，其活性显著下降(P<0.05)；乙醇+GE-132+VB₁+GSH组与乙醇组比较，肝线粒体酮锌超氧化物歧化酶(Cu,Zn-SOD)活性上升(P<0.05)。结论：GE-132与GSH和VB₁联合应用对乙醇导致的自由基损伤有协同保护作用。     

衰老大鼠某些脏器形态学和生物化学变化的实验观察

我们观察了老年大鼠的外部形态特征,白内障发生率,一些器官的组织学变化和细胞内脂褐素的积累情况,并以生物化学方法,测定了与心血管系统疾病、肿瘤,糖尿病等关系密切的纤维蛋白溶酶原激活物(Plasmino-gen Activators,PA)活性的变化。     

EFFECT OFYIN-INVIGORATING MIXTURE ON THECELLMEMERANEFLUIDITYOF NORMALANDMALIGNANTMUTANTCELLS

Observations were made on the effect ofYin-invigorating Mixture(YIM)(Ⅰ),DialysedYIM(Ⅱ) and YIM dlalysate(Ⅲ)on Labelednormal cell V_(79)and malignant mutant cellV_(79)-B_1 of Chinese hamster with fatty acid spinlabel compound(10.3).An increase of the orderparameter of FSR spectra was seen in V_(79)and V_(79)-B_1 cells after treatment with Ⅰ,Ⅱ andⅢ,with the peak at 55′.The increase in V_(79)-B_1was greater than that in V_(79).The dynamicvariations of ESR spectra also showed an in-crease of the order parameter with time,greaterin V_(79)-B_1 cells than in V_(79) cells within 235′.Theresults of the rotational correlation time of V_(79)cells revealed that only Ⅲappeared to havesignificant effect as compared with the untreat-ed control.No significant difference was seenamong the other groups.The results indicatethat YIM is effective in increasing the orderparameter and decreasing membrane fluidity inthe hydrophobie area of phospholipid moleculesin normal and malignant mutant lung cells ofthe Chinese hamster.The effect of YIM on thecell membrane do not appear to be closelyrelated to the K+,Na+,Ca+,Mg++,Mn++andZn++ the drug contains.The sites of action of and Ⅲ on layers in the cell membrane aredifferent,I acting mainly on the hydrophobicregion of the cell membrane and Ⅲ on thehydrophilic region of the cell membrane.