疤痕表皮移植术的临床应用

皮片移植术是烧伤后疤痕增殖挛缩病人后期整复治疗的重要方法,在正常皮肤切取中厚或全厚皮片移植于疤痕切除后的创面,以恢复功能和改善外貌。但是,在大面积烧伤的病人中,由于正常皮肤组织极少,无法取得足够的皮片用于后期整复手术,给病人的治疗带来极大的困难。为此,在烧伤病人的     

多爱肤敷料在中厚皮片供皮区的应用

目的探讨用多爱肤（DuoDerm）敷料作封闭疗法对中厚皮片供皮区创面愈合的作用。方法对52例封闭疗法病人和50例传统疗法病人的创面愈合时间、感染可能性及其疼痛程度进行对比评估。结果封闭疗法较传统疗法创面平均愈合时间短6．5d,创面疼痛程度低,未引起创面感染及其它不良反应。结论用多爱肤敷料做封闭疗法是一种安全、有效简便的方法。     

水通道蛋白1在急性坏死性胰腺炎大鼠胰腺组织的表达及其意义

目的 探讨水通道蛋白1(AQP1)在急性坏死性胰腺炎(ANP)大鼠胰腺的表达及其意义.方法 将48只雄性SD大鼠随机分为对照组和ANP组,制模后3、6、12、18 h各时间点分别处死6只.记录腹水量,测定血清淀粉酶;采用酶联免疫吸附试验(ELISA)检测血清AQP1含量;苏木素-伊红(HE)染色观察胰腺组织病理改变;伊文思兰染料(EB)血管外渗法检测胰腺组织毛细血管通透性;免疫组织化学和Westem blot法检测胰腺组织AQP1蛋白表达;荧光定量聚合酶链反应(PCR)检测AQP1基因mRNA表达.结果 (1)对照组3、6、12、18 h血清淀粉酶水平分别为(1308±759)、(1077±508)、(1325±761)、(1328±762)U/L,ANP组分别为(9102±2199)、(8799±1634)、(9398±1473)、(9484±862)U/L;对照组胰腺组织EB含量分别为(205.61±32.99)、(141.46±27.18)、(96.94±26.79)、(61.43±24.82)mg/L;ANP组分别为(273.59±23.47)、(253.51±31.68)、(221.15±73.68)、(185.28±42.35)mg/L;血清AQP1含量对照组为(74.08±11.80)、(78.49±9.06)、(75.77±7.37)、(72.75±13.87)mg/L,ANP组为(73.29±9.61)、(62.85±7.28)、(62.07±4.39)、(46.33±11.91)mg/L,两组比较差异均有统计学意义(P<0.01).(2)对照组3、6、12、18 h免疫组织化学灰度值分别为114.13±7.92、122.39±7.99、145.98±6.48、113.98±6.48,ANP组分别为80.07±14.89、110.54±4.45、103.77±10.48、99.18±6.95;对照组Western blot蛋白含量分别为1.19±0.33、1.02±0.25、0.90±0.33、1.06±0.20,ANP组分别为0.83±0.11、0.96±0.21、0.58±0.28、0.72±0.14.结果 均显示ANP组胰腺AQP1蛋白表达低于对照组(P<0.05);(3)对照组3、6、12、18 h荧光定量PCR检测ANP组胰腺AQP1基因mR-NA表达分别为2.13±0.63、2.02±1.40、2.07±0.86、2.49±2.47,ANP组为0.91±0.22、1.01±0.83、0.48±0.23、0.61±0.51,ANP组较对照组减弱(P<0.01).结论 ANP大鼠胰腺组织AQP1表达明显减弱,这可能在毛细血管渗漏综合征的发生中起重要作用.     

水通道蛋白1在急性坏死性胰腺炎大鼠胰腺组织的表达及其意义

Objective To study the expression of aquaporin 1 ( AQP1 ) in pancreas and its pathogenic role in rats with experimental acute necrotizing pancreatitis (ANP). Methods Forty-eight male Sprague-Dawley rats were randomly divided into two groups : control group ( n = 24 ) and ANP group ( n = 24). Six rats in each group were sacrificed at 3,6,12 and 18 h after induction of experimental models. Quantity of ascites and levels of serum amylases were measured at each time point. Serum AQPI was determined by ELISA. Pathological changes of pancreatic tissues were examined. Capillary permeability in pancreatic tissues was tested by Evans Blue (EB) extravasation method. AQPI expression in pancreatic tissues was detected by immunohistochemieal staining, Western blot and fluorescence quantitative polymerase chain reaction (FQ-PCR). Results (1) Serum amylase level at 3,6,12, and 18 h in control group was (1308±759) ,(1077±508), (1325±761),(1328±762) U/L,and that in ANP group was (9102± 2199), (8799±1634), (9398±1473), (9484±862) U/L respectively. The concentration of EB in pancreatic tissues at each time point in control group was (205.61±32.99), (141.46±27.18 ), (96.94± 26.79), (61.43±24.82) mg/L, and that in ANP group was ( 273.59±23.47 ), ( 253.51±31.68 ), (221.15±73.68 ), (185.28±42.35) ,respectively. There was significandy difference between two groups. Serum AQP1 level in control group at each point was ( 74.08±11.80), (78.49±9.06 ), (75.77± 7.37 ), ( 72.75±13.87 ) mg/L, and that in AN P group was (73.29±9.61 ), ( 62.85±7.28 ), (62.07± 4.39 ), (46.33±11.91 ) mg/L, respectively ( P < 0.01 ). ( 2 ) Protein expression of AQPI in pancreatic tissues detected by immunohistochemical staining in control group at each time point was 114.13±7.92, 122.39±7.99,145.98±6.48,113.98±6.48, and that in ANP group was 80.07±14.89,110.54± 4.45,103.77±10.48,99.18±6.95;Protein expression of AQP1 in pancreatic tissues detected by Western blot in control group at each time point was 1.19±0.33,1.02±0.25,0.90±0.33,1.06±0.20,and that in ANP group was 0.83±0.11,0.96±0.21,0. 58±0.28,0.72±0.14, respectively ( P < 0.05 ). ( 3 ) The mRNA level of AQP1 gene expressed in pancreas in control group at each time point was 0.91±0.22,1.01±0.83,0.48±0.23,0.61±0.51,and that in ANP group was 2.13±0.63,2.02±1.40, 2.07±0.86,2.49±2.47,respectively (P<0.01).Condusion The expression of AQP1 wasdown-reg-ulated in pancreas in rats with ANP,which might could play an important role in the pathogenesis of capil-lary leak syndrome.     

疤痕表皮移植术的临床应用

皮片移植术是烧伤后疤痕增殖挛缩病人后期整复治疗的重要方法,在正常皮肤切取中厚或全厚皮片移植于疤痕切除后的创面,以恢复功能和改善外貌.但是,在大面积烧伤的病人中,由于正常皮肤组织极少,无法取得足够的皮片用于后期整复手术,给病人的治疗带来极大的困难.为此,在烧伤病人的疤痕部位切取表皮片,移植于创面,取得良好的效果.     

硅酮喷雾剂在预防增殖性疤痕中的应用

为防止烧伤创面愈合后增殖性疤痕挛缩造成功能障碍和形态上受损，应用硅酮喷雾剂治疗患者141例，152个部位，观察3月以上均达到较好的效果，且使用简便。作者介绍了硅酮喷雾剂应用时间、方法等。     

闭孔疝6例临床诊治分析

<正>闭孔疝是一种临床较少见的疝,由于其临床表现不典型,临床医师对该病的认识不足,常常误诊,延误病情,导致病死率较高。为探讨闭孔疝的临床特征,提高临床医师对该     

水通道蛋白1在急性坏死性胰腺炎大鼠胰腺组织的表达及其意义

Objective To study the expression of aquaporin 1 ( AQP1 ) in pancreas and its pathogenic role in rats with experimental acute necrotizing pancreatitis (ANP). Methods Forty-eight male Sprague-Dawley rats were randomly divided into two groups : control group ( n = 24 ) and ANP group ( n = 24). Six rats in each group were sacrificed at 3,6,12 and 18 h after induction of experimental models. Quantity of ascites and levels of serum amylases were measured at each time point. Serum AQPI was determined by ELISA. Pathological changes of pancreatic tissues were examined. Capillary permeability in pancreatic tissues was tested by Evans Blue (EB) extravasation method. AQPI expression in pancreatic tissues was detected by immunohistochemieal staining, Western blot and fluorescence quantitative polymerase chain reaction (FQ-PCR). Results (1) Serum amylase level at 3,6,12, and 18 h in control group was (1308±759) ,(1077±508), (1325±761),(1328±762) U/L,and that in ANP group was (9102± 2199), (8799±1634), (9398±1473), (9484±862) U/L respectively. The concentration of EB in pancreatic tissues at each time point in control group was (205.61±32.99), (141.46±27.18 ), (96.94± 26.79), (61.43±24.82) mg/L, and that in ANP group was ( 273.59±23.47 ), ( 253.51±31.68 ), (221.15±73.68 ), (185.28±42.35) ,respectively. There was significandy difference between two groups. Serum AQP1 level in control group at each point was ( 74.08±11.80), (78.49±9.06 ), (75.77± 7.37 ), ( 72.75±13.87 ) mg/L, and that in AN P group was (73.29±9.61 ), ( 62.85±7.28 ), (62.07± 4.39 ), (46.33±11.91 ) mg/L, respectively ( P < 0.01 ). ( 2 ) Protein expression of AQPI in pancreatic tissues detected by immunohistochemical staining in control group at each time point was 114.13±7.92, 122.39±7.99,145.98±6.48,113.98±6.48, and that in ANP group was 80.07±14.89,110.54± 4.45,103.77±10.48,99.18±6.95;Protein expression of AQP1 in pancreatic tissues detected by Western blot in control group at each time point was 1.19±0.33,1.02±0.25,0.90±0.33,1.06±0.20,and that in ANP group was 0.83±0.11,0.96±0.21,0. 58±0.28,0.72±0.14, respectively ( P < 0.05 ). ( 3 ) The mRNA level of AQP1 gene expressed in pancreas in control group at each time point was 0.91±0.22,1.01±0.83,0.48±0.23,0.61±0.51,and that in ANP group was 2.13±0.63,2.02±1.40, 2.07±0.86,2.49±2.47,respectively (P<0.01).Condusion The expression of AQP1 wasdown-reg-ulated in pancreas in rats with ANP,which might could play an important role in the pathogenesis of capil-lary leak syndrome.     

冬凌草甲素通过上调miR-204-5p抑制PC-3细胞迁移与侵袭的作用机制研究

目的 探索冬凌草甲素影响PC-3细胞迁移及侵袭的作用及机制,为冬凌草甲素治疗前列腺癌骨转移提供前期体外实验参考。 方法 通过CCK8检测冬凌草甲素对PC-3细胞增殖的影响,Transwell检测冬凌草甲素对PC-3细胞迁移和侵袭的影响,qRT-PCR分析冬凌草甲素溶液处理PC-3细胞后miR-204-5p及NF-κB信号通路表达情况,Western blotting分析冬凌草甲素溶液处理PC-3细胞后NF-κB信号通路蛋白表达情况。 结果 冬凌草甲素溶液以浓度依赖性和时间依赖性方式抑制PC-3细胞的增殖,与对照组相比差异有统计学意义（P<0.01）,冬凌草甲素IC50值为10 μmol/L;冬凌草甲素溶液呈浓度依赖性抑制PC-3细胞的迁移和侵袭,差异较对照组有统计学意义（P<0.01）;冬凌草甲素溶液能促进PC-3细胞表达miR-204-5p并阻断NF-κB信号通路下游基因表达;过表达miR-204-5p和冬凌草甲素均能抑制PC-3细胞NF-κB信号通路下游基因表达。 结论 冬凌草甲素能抑制PC-3细胞的增殖、迁移及侵袭,其作用机制可能与其能上调miR-204-5p阻断NF-κB信号通路有关。     

老年腹股沟疝无张力修补术与传统修补术的比较

目的 探讨无张力腹股沟疝修补术与传统腹股沟疝修补术在老年患者中应用的疗效。方法 老年患者84例．随机分为两组．分别施行疝环充填式无张力修补术和传统腹股沟疝修补术．并随访2年。结果 无张力腹股沟疝修补术具有手术时间短，术后疼痛轻、恢复快、并发症少及复发率低等优点。结论 无张力腹股沟疝修补术符合腹股沟管的解剖结构和生理需要．老年患者尤为适用。