Signal transduction mechanisms involved in angiotensin-(1-7)-stimulated arachidonic acid release and prostanoid synthesis in rabbit aortic smooth muscle cells

This study investigated the signal transduction mechanisms of angiotensin-(1-7) [Ang-(1-7)]- and Ang II-stimulated arachidonic acid (AA) release for prostaglandin (PG) production in rabbit aortic vascular smooth muscle cells. Ang II and Ang-(1-7) enhanced AA release in cells prelabeled with [3H]AA. However, 6-keto-PGF1 alpha synthesis produced by Ang II was much less than that caused by Ang-(1-7). In the presence of the lipoxygenase inhibitor baicalein, Ang II enhanced production of 6-keto-PGF1 alpha to a greater degree than Ang-(1-7). Angiotensin type (AT)1 receptor antagonist DUP-753 inhibited only Ang II-induced [3H]AA release, whereas the AT2 receptor antagonist PD-123319 inhibited both Ang II- and Ang-(1-7)-induced [3H]AA release. Ang-(1-7), receptor antagonist D-Ala7-Ang-(1-7) inhibited the effect of Ang-(1-7), but not of Ang II. In cells transiently transfected with cytosolic phospholipase A2 (cPLA2), mitogen-activated protein (MAP) kinase or Ca(++)-/cal-modulin-dependent protein (CAM) kinase II antisense oligonucleotides, Ang-(1-7)- and Ang II-induced [3H]AA release was attenuated. The CaM kinase II inhibitor KN-93 and the MAP kinase kinase inhibitor PD-98059 attenuated both Ang-(1-7)- and Ang II-induced cPLA2 activity and [3H]AA release. Ang-(1-7) and Ang II also increased CaM kinase II and MAP kinase activities. Although KN-93 attenuated MAP kinase activity, PD-98059 did not affect CaM kinase II activity. Both Ang II and Ang-(1-7) caused translocation of cytosolic PLA2 to the nuclear envelope. These data show that Ang-(1-7) and Ang II stimulate AA release and prostacyclin synthesis via activation of distinct types of AT receptors. Both peptides appear to stimulate CaM kinase II, which in turn, via MAP kinase activation, enhances cPLA2 activity and release of AA for PG synthesis.     

Comparative study of conventional and magnetically coupled piezoelectric energy harvester to optimize output voltage and bandwidth

Energy harvesting has experienced significant attention from researchers globally. This is due to the quest to power remote sensors and portable devices with power requirements of tens to hundreds of μW. Hence, ambient vibration energy has the potential to provide such power demands. Thus, cantilever beams with piezoelectric materials have been utilized to transduce mechanical energy in vibrating bodies to electrical energy. However, the challenge is to develop energy harvesters that can harvest sufficient amount of energy needed to power wireless sensor nodes at wide frequency bandwidth. In this article, piezoelectric energy harvester (PEH) beams with coupled magnets are proposed to address this issue. With macro fiber composite as the piezoelectric transducer, mathematical models of different system configurations having magnetic couplings are derived based on the continuum based model. Simulations of the system dynamics are done using numerical integration technique in MATLAB software to study the influence of magnetic interactions in generating power and frequency bandwidth due to base excitations at low frequency range. Experimental results comparing conventional system and the proposed piezoelectric beam configurations with coupled magnets are also presented. Finally, the optimal beam separation distance between the magnetic oscillator and PEH is presented in this work.

     

Harvesting vibration energy using piezoelectric material: Modeling,simulation and experimental verifications

Piezoelectric materials produce electrical charges when subjected to dynamic strain. These materials can be used to capture and store vibrational energy which later can be used to power up small devices. This paper presents an analytical estimation of voltage production of piezoelectric cantilever beam when subjected to base excitation, with and without attached proof masses. The beam is modeled using Euler–Bernoulli, also known as thin beam theory. As such, the model obtained here is applicable for micro- and nano-beams. The frequency response function (FRF) that relates the output voltage and transverse acceleration is identified for multi-mode vibration. These analytical predictions are then compared with experimental results and good agreement is obtained.