Control of stroma-dependent hematopoiesis by basic fibroblast growth factor: stromal phenotypic plasticity and modified myelopoietic functions

The immunological agglutination reactions of physically absorbed F(ab')2 molecules onto anionic and cationic latex particles have been investigated by means of optical absorbance measurements. These measurements have been conducted under different conditions to determine the most influential factors. Surface F(ab')2 and BSA densities, particle concentration in the reaction medium and polyethylene glycol concentration are some of these factors. Sensitized cationic and anionic latexes differ considerably with respect to their colloidal stability and reactivity. As a general rule, the sensitized cationic latex has a relatively higher colloidal stability and hence, it provides reagents with a better optical response. Less than 0.025 microgram/ml of C-reactive protein has been detected using this particle enhanced optical immunoassay.     

On the hardness of approximating label-cover

The Label-Cover problem, defined by S. Arora, L. Babai, J. Stern, Z. Sweedyk [Proceedings of 34th IEEE Symposium on Foundations of Computer Science, 1993, pp. 724-733], serves as a starting point for numerous hardness of approximation reductions. It is one of six ‘canonical’ approximation problems in the survey of Arora and Lund [Hardness of Approximations, in: Approximation Algorithms for NP-Hard Problems, PWS Publishing Company, 1996, Chapter 10]. In this paper we present a direct combinatorial reduction from low error-probability PCP [Proceedings of 31st ACM Symposium on Theory of Computing, 1999, pp. 29-40] to Label-Cover showing it NP-hard to approximate to within 2^{(logn)1−o(1)}. This improves upon the best previous hardness of approximation results known for this problem.We also consider the Minimum-Monotone-Satisfying-Assignment (MMSA) problem of finding a satisfying assignment to a monotone formula with the least number of 1's, introduced by M. Alekhnovich, S. Buss, S. Moran, T. Pitassi [Minimum propositional proof length is NP-hard to linearly approximate, 1998]. We define a hierarchy of approximation problems obtained by restricting the number of alternations of the monotone formula. This hierarchy turns out to be equivalent to an AND/OR scheduling hierarchy suggested by M.H. Goldwasser, R. Motwani [Lecture Notes in Comput. Sci., Vol. 1272, Springer-Verlag, 1997, pp. 307-320]. We show some hardness results for certain levels in this hierarchy, and place Label-Cover between levels 3 and 4. This partially answers an open problem from M.H. Goldwasser, R. Motwani regarding the precise complexity of each level in the hierarchy, and the place of Label-Cover in it.     

A data-driven VLSI array for arbitrary algorithms

The design of specialized processing array architectures, capable of executing any given arbitrary algorithm, is proposed. An approach is adopted in which the algorithm is first represented in the form of a dataflow graph and then mapped onto the specialized processor array. The processors in this array execute the operations included in the corresponding nodes (or subsets of nodes) of the dataflow graph, while regular interconnections of these elements serve as edges of the graph. To speed up the execution, the proposed array allows the generation of computation fronts and their cancellation at a later time, depending on the arriving data operands; thus it is called a data-driven array. The structure of the basic cell and its programming are examined. Some design details are presented for two selected blocks, the instruction memory and the flag array. A scheme for mapping a dataflow graph (program) onto a hexagonally connected array is described and analyzed. Two distinct performance measures-mapping efficiency and array utilization-and some performance results are discussed     

Multiresolution Representation of 2D CSG Models

Multiresolution is currently one of the main schemes used in CAD modeling for representing objects, particularly when large-scale geometric data must be transferred interactively over a network, as in the case of collaborative design. Increasingly complex products and growing competition have turned design into a collaborative team effort. Furthermore, the widespread development of Internet viewers has also necessitated the transfer and display of large-scale CAD models over networks. In order to reduce the volume of transferred data, efforts have been made to transfer CSG (Constructive Solid Geometry) models rather than those based on B_rep (Boundary Representation). This paper presents an original new method for speeding up data transfer by using multiresolution CSG models at different levels of details (LOD). The multiresolution CSG algorithm generates a hierarchy of multiresolution CSG trees; at each level, the shape is further approximated and represented by a smaller number of CSG primitives. The paper analyzes the proposed algorithm, and demonstrates its feasibility.     

The Prominin-1-Derived Peptide Improves Cardiac Function Following Ischemia

Myocardial infarction (MI) remains the leading cause of death in the western world. Despite advancements in interventional revascularization technologies, many patients are not candidates for them due to comorbidities or lack of local resources. Non-invasive approaches to accelerate revascularization within ischemic tissues through angiogenesis by providing Vascular Endothelial Growth Factor (VEGF) in protein or gene form has been effective in animal models but not in humans likely due to its short half-life and systemic toxicity. Here, we tested the hypothesis that PR1P, a small VEGF binding peptide that we developed, which stabilizes and upregulates endogenous VEGF, could be used to improve outcome from MI in rodents. To test this hypothesis, we induced MI in mice and rats via left coronary artery ligation and then treated animals with every other day intraperitoneal PR1P or scrambled peptide for 14 days. Hemodynamic monitoring and echocardiography in mice and echocardiography in rats at 14 days showed PR1P significantly improved multiple functional markers of heart function, including stroke volume and cardiac output. Furthermore, molecular biology and histological analyses of tissue samples showed that systemic PR1P targeted, stabilized and upregulated endogenous VEGF within ischemic myocardium. We conclude that PR1P is a potential non-invasive candidate therapeutic for MI.     

Small‐angle X‐ray scattering study of modified porous suspension–poly(vinyl chloride) particles

Small‐angle X‐ray scattering (SAXS) was applied to investigate the microstructure of unmodified and modified porous commercial suspension‐type poly(vinyl chloride) (PVC) particles. The modified PVC particles were prepared by an in situ stabilizer‐free polymerization/crosslinking of particles absorbed with a monomer/crosslinker/peroxide solution. The modifying polymers include styrene with or without divinyl benzene (DVB) as a crosslinker and methyl methacrylate (MMA) with or without ethylene glycol dimethacrylate (EGDMA) as a crosslinker. The SAXS method was used to highlight the effect of polystyrene (PS) on the microstructure of PVC particles and to evaluate the characteristic lengths, both in the PVC/PS and the PVC/XPS (PS crosslinked with 0 and 5% DVB, respectively) systems. A model is suggested, where during the synthesis modification process, swelling of PVC by styrene and styrene polymerization occur simultaneously. PVC swelling by styrene causes destruction of the PVC subprimary particles, whereas styrene polymerization leads to phase separation resulting from incompatibility of the polymers. It was further suggested that because of PVC swelling by styrene, structure of the subprimary particles is lost. Therefore the characteristic lengths of PVC/PS and PVC/XPS, as calculated from the SAXS measurements, were attributed to the size of the phase‐separated PS and XPS inclusions, respectively. The SAXS method also shows that PMMA and XPMMA do not influence the PVC microstructure. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 95: 1024–1031, 2005     

Efficient model checking for LTL with partial order snapshots

Certain behavioral properties of distributed systems are difficult to express in interleaving semantics, whereas they are naturally expressed in terms of partial orders of events or, equivalently, Mazurkiewicz traces. Two examples of such properties are serializability of a database and global snapshots of concurrent systems. Recently, a modest extension for LTL by an operator that expresses snapshots, has been proposed. It combines the ease of linear (interleaving) specification with this useful partial order concept. The new construct allows one to assert that a global snapshot appeared in the past, perhaps not in the observed execution sequence, but possibly in an equivalent one.     

Safety evaluation of flashing amber operation at signalized intersections

The safety of a flashing amber signal for all directions at off-peak hours as a replacement for regular traffic signal operations was evaluated. The proposed control strategy was motivated by the need for energy conservation through reduced amounts of acceleration and of idling time of vehicles. The methodology of the study employed as a measure a broadened definition of "conflict" that freed observers from the need to detect only emergency evasive manoeuvres and decreased their subjective interpretations. Observations were carried out by trained observers at a sample of intersections, using two control strategies: full signal operation and flashing amber phase. Stationed at each leg of an intersection, the observers noted the travel direction of any two vehicles involved in a conflict. The results showed that the most frequent type of conflict under full signal operation was of the rear-end type; during the flashing amber operation, crossing and merging conflicts were dominant. It was concluded that up to a volume of 600 vehicles per hour, flashing operation does not increase the number of conflicts.     

Pre-tensioning of fabrics in cement-based composites

This paper studies the effects induced by applying a pre-tension load during the production of fabric-cement composites. The flexural behavior as well as the bonding between the different fabrics and the cement matrix was examined, as a means for characterizing the processing parameters. Microstructure characteristics of the fabric-matrix interface as well as the viscous elasticity properties of the fabrics were also explored and correlated with the mechanical properties of the composite. It was found that pre-tensioning of fabrics and the time at which the tension is removed can significantly influence the performance of the composite depending on yarn properties, mainly the viscous-elastic properties, and fabric geometry.