Gaussian beam ray-equivalent modeling and optical design

It is shown that the propagation and transformation of a simply astigmatic Gaussian beam by an optical system with a characteristic ABCD matrix can be modeled by relatively simple equations whose terms consist solely of the heights and slopes of two paraxial rays. These equations are derived from the ABCD law of Gaussian beam transformation. They can be used in conjunction with a conventional automatic optical design program to design and optimize Gaussian beam optical systems. Several design examples are given using the CODE-V optical design package.     

Impact of Ozonation Process on the Microbiological Contamination and Antioxidant Capacity of Highbush Blueberry (Vaccinum corymbosum L.) Fruit during Cold Storage

In the studies conducted, the impact of the innovative ozonation procedure on the microbial state and antioxidant potential of highbush blueberry (Vaccinum corymbosum L.) stored under cold storage conditions was assessed. Microbiological analysis was carried out to determine the total number of mesophilic aerobic bacteria and the total number of fungi during the storage experiment. In addition, changes in the flavonoid, anthocyanins, and vitamin C content and the total antioxidant capacity were monitored during the storage. The degree of fruit infection with gray mold and anthracnose was determined. It was found that daily ozonation of fruits with a dose of 15 ppm for 30 min, every 12 h, for 28 days effectively reduced the development of aerobic mesophilic bacteria and fungi. On the last day of storage, symptoms of the infection by gray mold were observed in 27.5% of the control fruit, while the absence of symptoms was observed in case of the ozonated fruit. On the other hand, ozone was ineffective in case of inhibiting the infection by anthracnose. Nevertheless, the ozonation process allowed maintaining a high antioxidant potential of the fruit and substantially reduced losses of flavonoids, anthocyanins, and vitamin C. The utilized procedure has proved to be effective, providing the possibility of extensive use of ozone as a factor allowing sustaining a high commercial and consumption value of the fruit over extended time.     

Reduction of Captan,Boscalid and Pyraclostrobin Residues on Apples Using Water Only,Gaseous Ozone,and Ozone Aqueous Solution

Intensive use of plant protection products in modern agriculture and horticulture often results in increasing residue levels of active ingredients of plant protection products in fruits and vegetables. Even if the maximum residue levels are not exceeded, the synergic effects of various compounds may have a serious impact on consumers' health. In particular, more sensitive consumer groups, e.g., children, may be affected. Therefore, it is important to develop an effective method that could be utilized for reduction of pesticide residue levels in food products of plant origin. In this work, possible application of ozone to reduce pesticide residue levels in apples has been investigated. The fruits were treated with ozone in gaseous state, in ozonized water, and in water alone, which was used for rinsing the fruits. The experiments included tests on apples that were subjected to a protection program using captan, boscalid and pyraclostrobin. The ozonized water and treatment with a gaseous ozone were found to be not more efficient in reducing the pesticide content on the fruits than simple washing procedures, e.g., 81–95% reduction of captan residue or 40–67% reduction of boscalid residue and 20–42% reduction of pyraclostrobin residue. Still, ozonation of water used for rinsing prevented fruits from consecutive contamination by pesticide residues present in water after several rinsing cycles. It was proven that application of ozonation process as a part of post-harvest treatment of apples may be beneficial for the fruit quality.     

Design and characterization of dispersion compensating fiber basedon the LP01 mode

The practical implementation of erbium-doped fiber amplifiers with gain at 1.55 μm allows long unrepeatered transmission distances. However, in order to realize high data rates over these distances with already installed standard single mode fiber, techniques must be found to overcome the pulse spreading due to the positive chromatic dispersion of the transmission fiber in this window, we review a compensation technique based on propagating the signals through a specially designed fiber with large negative dispersion for the LP₀₁ mode, thereby ending up with zero net pulse spreading. The basis of the concept are discussed and a key figure of merit for dispersion compensating devices is defined. The design and optimization of dispersion compensating (DC) fiber is described with special attention to practical concerns including packaging and manufacturability. We describe experimental fabrication results of DC fiber, results of using the fiber to make compact dispersion compensating modules, and the outcome of recent systems experiments incorporating the fiber     

Expanding the Scope of Sortase‐Mediated Ligations by Using Sortase Homologues

Sortase‐catalyzed transacylation reactions are widely used for the construction of non‐natural protein derivatives. However, the most commonly used enzyme for these strategies (sortase A from Staphylococcus aureus) is limited by its narrow substrate scope. To expand the range of substrates compatible with sortase‐mediated reactions, we characterized the in vitro substrate preferences of eight sortase A homologues. From these studies, we identified sortase A enzymes that recognize multiple substrates that are unreactive toward sortase A from S. aureus. We further exploited the ability of sortase A from Streptococcus pneumoniae to recognize an LPATS substrate to perform a site‐specific modification of the N‐terminal serine residue in the naturally occurring antimicrobial peptide DCD‐1L. Finally, we unexpectedly observed that certain substrates (LPATXG, X=Nle, Leu, Phe, Tyr) were susceptible to transacylation at alternative sites within the substrate motif, and sortase A from S. pneumoniae was capable of forming oligomers. Overall, this work provides a foundation for the further development of sortase enzymes for use in protein modification.     

Ion exchange kinetics in removal of small ions. Effect of salt concentration on inter- and intraparticle diffusion

Kinetics of ion removal from wastewaters by ion exchange on a commercial synthetic zeolite has been investigated. Dynamic breakthrough profiles have been registered and exploited to determine ion exchange equilibria and mass transport resistances.A simplified dynamic model based on the equation of solid-film driving force has been employed to determine coefficients of mass transport kinetics in the presence of different solutions of regenerating salt. Two diffusional resistances have been found to govern the mass transport kinetics: interparticle diffusion (external film diffusion) and the solid phase diffusion within micropores of the zeolite crystals. At low solution concentration interparticle mass transfer was dominant in the mass transport mechanism, whereas solid phase diffusion dominated at higher concentration. The influence of the salt concentration on mass transfer kinetics has been analyzed using the Nernst-Planck and the Maxwell-Stefan models.The kinetic and isotherm coefficients determined were used to simulate the cyclic process of ion removal: ion exchange and the column regeneration.To verify analysis a generalized dynamic model has been used accounting for directly all the diffusion resistances.     

Sand pads: A promising technique to quantify human visitation into nature conservation areas

In many places the expansion of urban areas has brought recreationists into close proximity to nature conservation areas, sometimes leading to conflict where recreation and sensitive environmental or natural values are incompatible. An important first step in managing these conflicts is to assess the degree and nature of the problem. We describe the application, and methodological considerations, associated with the use of an innovative, low-cost, practical technique to monitor human intrusions into a wetland reserve which has been designated as ‘off-limits’ to the general public. The use of seven frequently monitored sand pads over 13 weeks enabled us to determine that intrusions occurred in every week (3–14 per sand pad), deep inside the reserve during most weeks, and also identified the key access points. Most intrusions occurred during holiday periods and were by walkers or cyclists. We also conducted a series of simple experiments to examine the utility of sand pads. Our sand pads maintained their shape well and held footprints for over 1 month, they were rarely avoided by people and provided reliable indices of the level of human activity. Sand hardness varied with rainfall, and hardened sand was frequent (53.8% of 26 days) and potentially prevented detection of people. We conclude that the sand pad technique is an effective and efficient tool to measure recreational use of off-limits areas and other conservation and recreation areas, provided human traffic is not too intense, and that checks are made reasonably frequently.     

Design of continuous ion exchange process for the wastewater treatment

A generic design procedure for the continuous ion exchange process is proposed. The procedure is based on the optimized arrangement of parallel batch columns. The continuity of the process is achieved by proper shifting of the inlet conditions. The method for the optimization of the process variables is presented.The concept is demonstrated on the example of ammonia removal from wastewaters. Two flowsheet schemes are considered utilizing fresh or recycled regenerating agent.The superiority of the optimized process over the periodic operation is demonstrated.     

Strong Minimax Lower Bounds for Learning

Minimax lower bounds for concept learning state, for example, that for each sample size n and learning rule g _n , there exists a distribution of the observation X and a concept C to be learnt such that the expected error of g _n is at least a constant times V/n, where V is the vc dimension of the concept class. However, these bounds do not tell anything about the rate of decrease of the error for a fixed distribution-concept pair.In this paper we investigate minimax lower bounds in such a (stronger) sense. We show that for several natural k-parameter concept classes, including the class of linear halfspaces, the class of balls, the class of polyhedra with a certain number of faces, and a class of neural networks, for any sequence of learning rules {g _n }, there exists a fixed distribution of X and a fixed conceptC such that the expected error is larger than a constant timesk/n for infinitely many n. We also obtain such strong minimax lower bounds for the tail distribution of the probability of error, which extend the corresponding minimax lower bounds.