Pulsed cold plasma-induced blood coagulation and its pilot application in stanching bleeding during rat hepatectomy

This paper presents plasma-induced blood coagulation and its pilot application in rat hepatectomy by using a home-made pulsed cold plasma jet. Experiments were conducted on blood coagulation in vitro, the influence of plasma on tissue in vivo, and the pilot application of rat hepatectomy. Experimental results show that the cold plasma can lead to rapid blood coagulation. Compared with the control sample, the plasma-induced agglomerated layer of blood is thicker and denser, and is mostly composed of broken platelets. When the surface of the liver was treated by plasma, the influence of the plasma can penetrate into the liver to a depth of about 500 μm. During the rat hepatectomy, cold plasma was proved to be effective for stanching bleeding on incision. No obvious bleeding was found in the abdominal cavities of all six rats 48 h after the hepatectomy. This implies that cold plasma can be an effective modality to control bleeding during surgical operation.     

LCR触发多级多通道火花开关的设计及其性能分析

为了进行电粉碎岩石和等离子体灭菌的实验研究,研制了一种LCR触发多级多通道火花开关。采用μs上升前沿的初级脉冲充电电源对低电感陶瓷电容充电,以火花开关作为短脉冲形成开关。在600Ω负载电阻下,测试了火花开关的工作性能。利用脉冲电源开展水中灭菌实验,考察了开关的工作性能。实验发现:当气体间隙总长为14mm时,开关的导通电压范围为29～45kV。当火花开关的工作电压为34kV时,火花开关平均导通时延和抖动分别为115μs和71μs;负载上峰值功率为1.776MW;电阻上获得的电压波形基本一致,电压幅值在33.4～34kV范围内,电压的上升时间范围为23～28ns;开关的能量损耗为8%,其中触发电路引起的能量损耗为2.163%。火花开关在累计工作2.5万次后,电极烧蚀均匀,厚度无变化。火花开关分别在有、无LCR触发电路情况下连续运行20次的对比实验证明,LCR触发方法能够显著提高多级多通道的火花开关工作的稳定性。     

Experimental study on bacteria disinfection using a pulsed cold plasma jet with helium/ oxygen mixed gas

Cold atmospheric plasma jet is widely used in many fields due to the reactive oxygen species and low temperature for heat-sensitive products. This paper presents the inactivation of bacteria via a pulsed plasma jet with He/O₂ mixed gas. To evaluate the disinfection performance, Staphylococcus aureus was used as an indicator bacteria for experiments. When the plasma jet dealt with agar plates spraying bacteria, it was found that mixed gas has a better performance than pure inert gas, indicated by the disinfection area. The increment of oxygen gas addition was beneficial to the disinfection ability of the plasma jet, while the gas had an opposite effect on the length of jet production. The experiments showed the efficacy of Staphylococcus aureus disinfection could reach up to 99.47% via a helium/oxygen (2%) plasma jet.