复方甘草酸苷治疗系统性红斑狼疮的疗效观察

系统性红斑狼疮( SLE)是一种累及多个系统、多个脏器的自身免疫性疾病,临床表现复杂,病程迁延、反复.皮质类固醇激素在目前仍然是治疗 SLE的一线药.复方甘草酸苷注射液(美能,日本美能发源制药公司生产,深圳健安医药有限公司总经销)具有一定的皮质类固醇激素样作用,笔者以其配合皮质类固醇激素治疗 SLE30例,取得一定疗效,现将观察结果报告如下.     

γ-干扰素加维A酸霜治疗扁平疣临床疗效观察

目的 :了解γ -干扰素加维A酸霜治疗扁平疣的疗效。方法 :选择360例门诊诊断明确的扁平疣患者 ,随机分为两组 ,治疗组γ-干扰素100万U肌注加0 025 %迪维霜外用 ,对照组0 025%迪维霜外用 ,进行对照治疗。结果 :治疗组总有效率为93 9% ,显效率为88 3% ,痊愈率为40 1% ,对照组总有效率为77 8 % ,显效率为48 9 % ,痊愈率为18 9% ,两组间总有效率、显效率、痊愈率经卡方检验 ,P<0 05,疗效差异有显著性 ,均无严重不良反应。结论 :γ -干扰素配合维A酸霜外用治疗扁平疣具有高效、起效快、疗程短、安全性好等优点。     

湿疹护理比用药更重要

湿疹是多种因素引起的具有多形性皮损和明显渗出倾向的皮肤炎症性疾病。湿疹一词源于希腊文ekzein,意为”沸腾或起泡”。湿疹的好发季节为春季、夏季和秋季。急性期主要表现为密集的粟粒大小的丘疱疹或小水疱．部分可能有大疱,基底部潮红,抓挠致丘疱疹、水疱破裂后．可出现小的糜烂面,渗出倾向明显;亚急性期表现为小丘疹、鳞屑及结痂．仅有少量丘疱疹、小水疱及糜烂：慢性期主要表现为皮肤增厚、色素沉着、表面粗糙,覆以少许鳞屑或结痂。     

磷酸化STAT3和磷酸化ERK1/2在皮脂腺癌中的过度表达

目的:了解磷酸化细胞信号传导与转录活化因子3(STAT3)及磷酸化细胞外信号调节激酶(ERK1/2)蛋白在皮脂腺癌中表达的意义。方法:采用免疫组化ABC的方法检测了磷酸化STAT3和磷酸化ERK1/2蛋白在17例皮脂腺癌和20例皮脂腺瘤包埋切片中的表达。结果:在17例皮脂腺癌包埋切片中有14例p-STAT3表达阳性,13例p-ERK1/2表达阳性,而在20例皮脂腺瘤中有2例p-STAT3表达阳性,3例p-ERK1/2表达阳性;p-STAT3和p-ERK1/2在皮脂腺癌中的阳性表达明显高于它们在皮脂腺瘤中的阳性表达(均为P<0.01)。结论:p-STAT3和p-ERK1/2在皮脂腺癌的肿瘤形成机制中起重要作用。     

Construction of Prokaryotic Expression Plasmid of mtrC Protein of Neisseria gonorrhoeae and Its Expression in E. Coli

Summary： In order to provide a rational research basis for detection of resistance of Neisseria gonorrhoeae to antimierobial hydrophobie agents and study on the resistant mechanism of multiple transferable resistance （mtr） efflux system, plasmid pET-28a（＋） encoding mtrC gene was constructed and the related target protein was expressed in Escherichia colt （E. cold DE3. The fragments of mtrC gene of Neisseria gonorrhoeae from the standard strains were amplified and cloned into prokaryotic expression plasmid pET-28a（＋） with restriction endonuelease to construct recombinant pET-mtrC which was verified by restriction endonuelease and DNA sequencing. The recom- binant was transformed into E. coli DE3 to express the protein mtrC induced by IPTG. The results showed mtrC DNA fragment was proved correct through restriction endonuelease and DNA sequencing. hs sequence was 99.5 % homologus to that published on GeneBank （U14993）. A 48.5 kD fusion protein which was induced by IPTG was detected by SDS-PAGE. It was concluded that the construction of prokaryotic expression plasmid of mtrC protein of Neisseria gonorrhoeae was correct and the fusion protein was successively expressed in E. coli.     

不同病程尖锐湿疣患者血清γ干扰素、白介素-2水平的研究

目的 :研究不同病程尖锐湿疣 (CA)患者血清γ干扰素 (IFN -γ)、白介素 - 2 (IL - 2 )水平的变化在CA免疫发病机制中的作用。方法 :采用双抗体夹心ELISA法 ,检测了 6 0例不同病程CA患者血清IFN -γ、IL - 2水平。结果 :CA患者组血清IFN -γ、IL - 2水平明显低于健康对照组 (P <0 0 1) ,而CA长病程组血清IFN -γ、IL- 2水平明显低于CA短病程组 (P <0 0 1)。结论 :CA患者有TH1型细胞因子 (如 :IFN -γ、IL - 2 )表达低下 ,长病程患者更明显 ,患者TH1型细胞因子表达低下在CA的免疫发病机制中可能起重要作用。     

肾移植术后全身泛发性单纯疱疹1例

患者，男，43岁，农民。因“右小腿皮疹伴瘙痒20余天，泛发全身5天”，于2003年8月27日入院。患者20天前无明显诱因右小腿外侧发生水疱，伴瘙痒，未曾治疗，3天后形成脓疱，约1周后脓疱穿破后形成溃疡，伴疼痛，近5天全身出现散在性分布的水疱、脓疱及溃疡，无发热、头痛、关节疼痛等不适。发病以来，患者精神欠佳，饮食、睡眠、大小便正常。患者曾于2003年6月26日因肾功衰竭在本院泌尿外科行右侧肾移植手术。     

内皮素对黑素瘤A375细胞转化生长因子-β1及磷酸化Smad 3蛋白表达的调节

Objective To investigate the effects ofendothelin-1 (ET-1) and endothelin-3 (ET-3) on the expression of transformation growth factor-beta 1 (TGF-β1) and phosphorylation of Smad 3 in malignant melanoma cell line, A375. Methods Cultured A375 cells were classified into 5 groups, i.e. control group (no stimulation), ET-1 group (stimulated with ET-1), ET-1+BQ123 group (treated with ET-1 and BQ123),ET-1 + BQ788 group (treated with ET-1 and BQ788), ET-3 group (stimulated with ET-3), to receive different stimulation. The working concentrations were 0, 0.1, 1, 10, 100 nmol/L for ET-1 and ET-3, 10 μmol/L for BQ123 and BQ788. After another 12- and 24-hour culture, ELISA, RT-PCR and Western blot were used to detect the expression of TGF-β1 protein and mRNA as well as phosphorylated Smad 3 (P-Smad 3). Results The expression of TGF-β1 in A375 cells was up-regulated by ET-1, but down-regulated by ET-3, and both of the effects were in a concentration-dependent manner. Under the stimulation with ET-1 and ET-3 of 100 nmol/L, the level of TGF-β1 reached 1289.38 ± 89.42 ng/L per 105 cells and 85.09 ± 9.37 ng/L per 105 cells, respectively, significantly different from that in unstimulated cells (both P < 0.05). BQ123 signifi-cantly blocked the up-regnlatory effect of ET-1 on the expression TGF-β1 protein(P < 0.05), but BQ788 had no significant influence on the effect, so was the case with TGF-β1 mRNA. Western blot revealed that ET-1significantly elevated the expression of P-Smad 3 in A375 cells (P <0.05), and the elevation was significantly inhibited by BQ123, but not by BQ788. The expression of P-Smad 3 was statistically decreased by ET-3 in A375 cells (P <0.05). Conclusions The expression of TGF-β1 could be enhanced by ET-1, but suppressed by ET-3. It is likely that endothelin receptor A mediates the phosphorylation of Smad 3 induced by ET-1.     

皮肤肿瘤及正常皮肤组织中端粒酶活性的表达

目的 检测皮肤肿瘤及正常皮肤组织中端粒酶活性的表达。方法 采用端粒重复扩增TRAP及PCR方法，检测28例皮肤肿瘤(包括良性、癌前病变及恶性肿瘤)及15例正常皮肤组织中端粒酶活性。结果 15例正常皮肤中端粒酶表达仅为13．3％。28例皮肤肿瘤中有端粒酶表达的为82．1％，其中恶性肿瘤的端粒酶活性明显高于良性、癌前病变者。结论 端粒酶可能是皮肤肿瘤癌变中的一个早期事件，与皮肤癌变的发生发展过程有一定关系，可以反映组织的增生程度。     

不同病程尖锐湿疣IFN—γ、lL—2及lL—4水平的研究

目的 研究不同病程尖锐湿疣(CA)患者血清γ干扰素(IFN—γ)、白细胞介素-2(IL-2)及白细胞介素-4(IL-4)水平的变化在CA免疫发病机制中的作用。方法 采用双抗体夹心ELISA法，检测了60例不同病程CA患者血清IFN—γ、IL-2及IL-4水平。结果 CA患者组血清IFN—γ、IL-2水平明显低于健康对照组(P＜0．01)，而CA长病程组血清IFN—γ、IL-2水平明显低于CA短病程组(P＜0．01)；CA患者组血清IL-4水平明显高于健康对照组(P＜0．01)，而CA长病程组血清IL-4水平明显高于CA短病程组(P＜0．01)。结论 CA患者有Thl型细胞因子表达低下和Th2型细胞因子表达亢进，长病程患者更明显，患者Thl型细胞因子表达低下和Th2型细胞因子表达亢进在CA的免疫发病机制中可能起重要作用。