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QY Liu AE Schaffner YH Chang K Vaszil JL Barker 《Canadian Metallurgical Quarterly》1997,33(6):848-864
Embryonic rat hippocampal neurons were cultured in a serum-free defined medium (MEM/N3) either directly on poly-D-lysine (PDL) or on a confluent monolayer of postnatal cortical astrocytes, C6 glioma cells, or Rat2 fibroblasts. Neurons on PDL were grown in MEM/N3 or in MEM/N3 conditioned for 24 h by astrocytes or C6 cells. Membrane capacitance (Cm) and gamma-aminobutyric acid (GABA)-, glycine-, kainate-, and N-methyl-D-aspartate (NMDA)-induced currents were quantified using whole-cell patch-clamp recordings. Cm as well as the amplitude and the density of these currents in neurons cultured on astrocytes were significantly greater than those in neurons grown on PDL after 24 and 48 h. C6 cells mimicked astrocytes in promoting Cm and GABA-, glycine-, and NMDA-evoked, but not kainate-evoked, currents. Cm and currents in neurons grown on Rat2 cells were comparable to those in neurons on PDL. Astrocytes maintained in culture for 3 months were noticeably less effective than freshly prepared ones just grown to confluence. Suppression of spontaneous cytoplasmic Ca2+ (Ca[c]2+) elevations in astrocytes by 1,2-bis(2-aminophenoxy) ehane-N, N, N, N-tetraacetic acid acetoxymethyl ester (BAPTA-AM) loaded intracellularly blocked the observed modulatory effects. Medium conditioned by either astrocytes or C6 cells mimicked the effects of direct coculture of neurons on these cells in promoting Cm and amino acid-evoked currents. Inclusion of antagonists at GABA and glutamate receptors in coculture experiments blocked the observed effects. Thus, diffusible substances synthesized and/ or secreted by astrocytes in a Ca(c)2+-dependent manner can regulate neuronal growth and aminoacid receptor function, and these effects may involve neuronal GABA and glutamate receptors. 相似文献
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S. C. Hogg H. V. Atkinson P. Kapranos 《Metallurgical and Materials Transactions A》2004,35(3):899-910
The rheology of Al-30Si-5Cu and Al-30Si-5Cu-2Mg spray-formed alloys has been characterized after holding at various times
and temperatures, using semisolid rapid compression to obtain load-displacement curves. Microstructures were examined after
partial remelting and compression in an attempt to understand the flow behavior. The initial resistance to flow generally
increased with holding time at temperature. This increase was attributed to the increased strength of a three-dimensional
(3-D) solid silicon network present in these alloys in the semisolid state, with the initial resistance to flow smaller in
the magnesium-containing alloys. With increased temperature, the initial resistance decreased due to increased liquid content
and grain spheroidization. The viscosity vs shear rate response was studied using the approach of Laxmanan and Flemings. The materials shear thinned rapidly during the
test conditions, in which the shear rate rapidly increased from approximately 2.5 to 92 s−1. The results were fitted to a power-law model, and the viscosities showed a drop of approximately two to four orders of magnitude
when extrapolated to a shear rate of 1000 s−1 (a typical value in actual component manufacture). The rapid compression test, which is close to conditions present in component
manufacture, was shown to have more in common with a shear rate jump experiment, rather than steady-state viscometry. 相似文献
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Simon Atkinson 《Sealing Technology》2003,2003(2):10-11
This case study covers what is believed to be the world’s largest dry-running, non-contacting gas seal, manufactured by John Crane. Recently returned for routine maintenance, it has reached its five-year anniversary while remaining in excellent condition. 相似文献
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HJ Lee HK Ha MH Kim YK Jeong PN Kim MG Lee JS Kim DJ Suh SG Lee YI Min YH Auh 《Canadian Metallurgical Quarterly》1997,169(2):517-520
OBJECTIVE: The purpose of this study was to evaluate ERCP and CT findings of ectopic drainage of the common bile duct into the duodenal bulb. CONCLUSION: Although rare, the diagnosis of ectopic drainage of the common bile duct into the duodenal bulb is important to prevent inadvertent damage during biliary tract or gastric surgery and to clarify the cause of chronic peptic ulcers. 相似文献
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We present a technique for in situ lens nucleus emulsification using low phaco power and high vacuum, a continuous curvilinear capsulorhexis, and hydrodelineation. Emulsification is done with the phaco tip slanted down 30 or 45 degrees. Cutting and aspiration do not cause an undesirable energy loss. This technique can be combined with the nuclear chopping or divide and conquer methods because of its ability to drill and hold the nucleus. Posterior capsular rupture is prevented because the separated epinucleus acts as a barrier between the nucleus and the cortex. The low power used minimizes the energy transfer to the corneal endothelium. This technique is particularly useful in eyes with brunescent cataract. 相似文献