老年梗阻性结肠癌临床特点及手术治疗分析

目的探讨老年梗阻性结肠癌患者的临床特点以及手术治疗的临床效果。方法回顾性分析47例老年梗阻性结肠癌患者手术治疗的临床资料。结果 45例痊愈出院,2例死亡,死亡率4.26%;术后并发症18例,发生率38.3%。结论梗阻性结肠癌老年患者误诊率高、并发症多,手术切除直接快速去除病灶,是临床治疗的主要手段。     

Identification of an 80 K Dalton Human Sperm Antigen

Monoclonal antibodies raised against human sperm were used as a probe to look for specific human sperm antigen. By “ Western blotting” ,an 80 K dalton molecular weight protein was recognized by the monoclonal antibody, 21-2-4. By the SDS polyacrylamide gel electrophoresis, the 80 Kd was the major band of deoxycholate extract in which this protein could be isolated from other components of sperm by eleetro-elution from the gel slab. For the purpose of evaluating the function of 80 Kd in fertilization, the rabbit anti-80 Kd polyclonal antibodies were produced and immunoassays were performed. The TAT result showed that the agglutination in patterns of tail to tail and head to head were induced by incubating human sperm with rabbit anti-8OKd antiserum. The SIT result showed that the human sperm motility was strongly inhibited by rabbit anti-80 Kd antiserum. The immanofluorescence staining of human sperm incubated with rabbit anti-80 Kd antiserum showed very strong fluorescence on postaerosome and tail. These results suggest that 80 Kd is a specific sperm antigen which locates on the surface of human sperm and possibly plays an important role in fertilization.     

早期乳腺癌老年患者保乳手术治疗效果观察

目的探讨早期乳腺癌老年患者保乳手术的临床效果。方法回顾性分析45例早期乳腺癌老年患者保乳手术的临床资料。结果手术顺利完成,平均出血量60 ml,随访6～24个月,1例复发,3例因非肿瘤原因死亡,其他患者无肿瘤复发和淋巴转移,乳房外形满意度95.56%。结论保乳手术疗效显著,术后并发症少、恢复快,适合早期乳腺癌老年患者。     

甲状腺癌再次手术的临床分析

目的探讨甲状腺癌再次手术的原因及治疗方法。方法对2006年8月至2010年12月我院25例甲状腺癌再次手术患者临床资料进行回顾性分析。结果行甲状腺次全切除+淋巴清扫术22例,行甲状腺全切除+淋巴清扫术3例,2例发生喉返神经损伤,其余患者均顺利结束手术。除1例于术后3年出现对侧的淋巴结转移,无患者复发和转移。结论误诊是甲状腺癌再次手术的主要原因,必须提高诊断的准确率,推广术中快速冰冻病理检查的方法,为选择合适的术式提供重要的参考依据。     

分离人类空肠弯曲菌两种培养方法的比较

空肠弯曲菌是人类肠炎的主要病原菌,其生长条件要求严格,在有氧(21％O_2)或厌氧环境中均不生长,需在减低氧压的微需氧环境中(含5％O_2)才能生长,故培养时需特殊装置。期望用烛缸法进行培养以代替含5％O_2的充气缸(下称气缸法)已成为分离本菌的研究方向。Luechtefeld等利用气缸法和烛缸法进行了动物粪便标本的对比试验。我室曾应用改良的Campy-BAP     

成人空肠弯曲杆菌肠炎(附7例报告)

我们在1981年7月至9月对182例成人急性腹泻患者无选择地进行粪便培养,其中7例分离到空肠弯曲杆菌,阳性率为3.85%。7例中仅有1例发热,腹痛一般不剧,大便多为黄水样,无1例血便。     

80K道尔顿的人精子抗原的鉴定

<正> 本文以抗人精子单克隆抗体作为探针进行人精子特异抗原的寻找工作。用转移免疫印迹法(Western blotting),已由单抗21-2-4识别出一分子量为80K道尔顿的精子蛋白。用人精子去氧胆酸钠提取物走的SDS-聚丙烯酰胺电泳显示80kd是提取物中的主要成分。电泳后将含有8Okd的胶条切下来,再用电泳洗脱法(Electro-elution)可将其回收纯化。为了评价80kd在生殖过程中     

大花罗布麻叶的药理学研究——Ⅱ大花罗布麻叶延缓衰老作用的实验观察

大花罗布麻叶(Poacgnam hendlrsonii(Hook.f.)Woodson)的乙醇提取物(P.H.I)可延长果蝇寿命,促进动物免疫功能,降低血清过氧化脂质水平,减少人工白内障的形成。体外试验有明显的抗自由基作用,经6个月长期毒性试验未见有明显的毒性反应,也未见有致突变现象,结果提示大花罗布麻叶有可能作为一种安全而有效的延缓衰老药物。     

Characterization of Monoclonal Antibodies against Human Sperm

Seven McAbs raised against human sperm were evaluated by several immunological assays. The results are shown as follows: (1) Among 7 MeAbs, 5 were IgG1, 2 were IgM.(2) By immunofluorescent assay, McAb 21-2-4 was localized on postacrosome, neck and midpiece of human sperm; other 5 McAbs were bound to plasma membrane and acrosome; another McAb gave a negative reaction. In addition, when McAbs were reacted with sperms of other species, positive results were also obtained, but with different patterns of staining. It is suggested that the location of the same antigen on sperms of various species may be different. (3) Five McAbs showed mixed pattern of agglutination in TAT, other two displayed tail-tail pattern; three McAbs gave pasitive test in SIT, two revealed weak positive reaction. (4) Cross-reactivity of seven McAbs was examined with seven human somatic tissues as well as peripheral blood lymphocytes, three McAbs reacted with lymphocytes of spleen, thymus and peripheral blood. (5) The reaction of McAbs with testes of various species was observed. McAb 21-2-4 reacted with germ cells of rabbit and hamster, and A 14 with germ cells of rat and hamster. Negative reaction of these two MeAbs with human testes was obtained, but they did react with ejaculatory, sperms, it is suggested that antigens reacted with McAbs probably, were coating antigens.     

空肠/结肠弯曲菌的血清学分型

按Penner血清分型方法,应用被动血凝试验对349株空肠/结肠弯曲菌进行了分型,其中病人287株,可分型208株(72.5%);健康带菌者17株,可分型11株(64.7%);动物45株,可分型32株(71.1%)。说明Penner血清分型法在我国基本适用,但我国还有新的血清型存在。可分型的菌株共达57个不同的血清型,与国外集中于2、4和3型的报道不一致。从动物菌株的血清分型提示鸡与人的关系比较密切,可能是人类空肠弯曲菌肠炎的主要传染源之一。