盐酸克伦特罗体外抑制大鼠胚胎肢芽细胞增殖和分化的研究

目的探讨盐酸克伦特罗对大鼠胚胎肢芽细胞的影响。方法采用大鼠胚胎肢芽细胞培养方法,观察盐酸克伦特罗对大鼠胚胎肢芽细胞增殖和分化的影响。结果盐酸克伦特罗对大鼠胚胎肢芽细胞增殖、分化均有抑制作用;细胞增殖:y^=0.507-0.000 7x,r=-0.949(P<0.01);细胞分化:y^=87.63-0.286x,r=-0.945(P<0.01),存在明显的剂量—反应关系,并呈显著性负相关;胚胎肢芽细胞半数抑制细胞增殖浓度(IP50)为0.34μg/L,半数抑制分化浓度(ID50)为0.09μg/L,IP50/ID50=3.78。结论盐酸克伦特罗可抑制大鼠胚胎肢芽细胞增殖和分化作用。     

硒多糖对镉中毒大鼠肾保护作用的研究

目的 探讨硒多糖对镉中毒大鼠肾的保护作用。方法 经腹腔注射2．5mg／kg．d氯化镉，连续3d，诱发大鼠肾中毒，同时给大鼠饮用不同剂量的硒多糖水溶液30d，分别测定肾组织匀浆的SOD、GSH-Px的活性，MDA含量，并同时观察大鼠肾组织的形态。结果 硒多糖能明显提升SOD、GSH-Px的括性，显著降低MDA含量，使大鼠肾脏维持正常的生理功能及组织结构。结论 硒多糖对镉中毒大鼠肾损伤有一定的保护作用。     

盐酸克伦特罗对大鼠SOD、MDA及免疫器官的影响

目的研究盐酸克伦特罗对大鼠血清SOD活性、MDA含量及免疫器官的影响。方法每天给大鼠分别经口一次染毒30、15、7．5mg／kg的盐酸克伦特罗，同时设对照组，连续6d，第7d颈动脉取血并处死动物，测定血清中SOD（超氧化物歧化酶）活性、MDA（丙二醛）含量，观察对免疫器官的影响。结果盐酸克伦特罗可使大鼠血清中SOD活性显著降低，MDA含量明显升高，与对照组比较P〈0．05或P〈0．01，并对胸腺和脾脏产生不同程度的病理损伤。结论盐酸克伦特罗可破坏机体氧化与抗氧化的动态平衡，并损及机体免疫器官的结构和功能。     

亚硒酸钠对大鼠胚胎肢芽细胞增殖和分化的影响

目的 探讨亚硒酸钠对大鼠胚胎的致畸性。方法 采用胚胎肢芽细胞培养方法，观察亚硒酸钠对大白鼠胚胎肢芽细胞增殖和分化的影响。结果 试验显示亚硒酸钠对大鼠肢芽细胞半数抑制分化浓度（ID50）为10．92μmol/L，半数抑制细胞增殖浓度（IP50）为7．95μmol/L。结论 亚硒酸钠的IP50／ID50值为0．728（＜6）,表明亚硒酸钠只是一种细胞毒性物质，而非致畸物。     

降钙素在糖尿病骨质疏松症治疗的应用研究

骨质疏松症（OP），1997年中国发生率为8390万人，占全国总人口数的6．6％，到2010年约为11040万人，8．2％。文旨在观察降钙素治疗。     

诊断“慢性支气管炎”要慎重

自2004年至2006年我院收治的所谓“慢性支气管炎”病人160例，其中被确诊为慢性支气管炎的病人只有40例，支气管炎扩张20例，咳嗽变异型哮喘30例，嗜酸细胞行支气管炎25例，鼻后滴液综合征25例．胃食管返流性咳嗽15例，吸烟5例。为什么会出现这种错误呢？是因为许多医生没有充分理解慢性支气管炎的定义。慢性支气管炎的定义为：慢性咳嗽、咳痰连续2年以上，每年至少3个月，排除其他心肺疾病，临床上很多医生没有意识到排除其他疾病蕴含的真正意义。只要是慢性咳嗽，X线检查没有明显异常，就给病人戴上“慢性支气管炎”这顶帽子。真所滑“慢性支气管炎是个筐．什么都往里面装。所以我们在诊治慢性咳嗽病人时要慎重，要注意以下几个方面的问题。     

二吲哚甲烷诱导荷鼻咽癌裸鼠肿瘤转归及对MDA、SOD和GSH-Px的影响

[目的]探讨3,3-二吲哚基甲烷(3,3-diindolylmethane,DIM)对荷鼻咽癌裸鼠肿瘤体积、丙二醛(Malondiadehycle,MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力及凋亡相关蛋白-半胱天冬酶-3信号分子的影响.[方法]采用完全随机的方式将48只BALB/C裸小鼠分为DIM低、中、高(0.004 g/kg.b.w、0.02 g/kg.b.w、0.1 g/kg.b.w)剂量组和溶剂对照组(体积分数为0.5%的羧甲基纤维素钠溶液),分别经口灌喂给予实验动物相应剂量的DIM或对照溶液10 d后,于其背部靠腋窝皮下接种人鼻咽癌细胞株CNE1,继续经口灌喂给予实验动物相应剂量的DIM或对照溶液.实验第30天时处死动物,计量肿瘤体积、留取肿瘤组织进行MDA含量、SOD和GSH-Px活力测定、活化型半胱天冬酶-3活性检测.[结果]DIM各剂量可不同程度地抑制肿瘤生长.与对照组比较,高剂量组SOD活力明显升高(P<0.05)、MDA含量明显降低(P<0.05)、GSH-Px活力明显升高(P<0.05).细胞凋亡的关键执行者活化型半胱天冬酶-3(Cleaved Caspase-3)表达随染毒剂量的增加逐渐增强(P<0.05).[结论]3,3-二吲哚基甲烷可抑制肿瘤生长,其机理可能与通过降低MDA含量,提高SOD活力、GSH-Px活力等途径降低氧化应激程度及通过Caspase-3信号分子诱导凋亡的作用相关.     

吲哚-3-甲醇对荷鼻咽癌裸鼠肿瘤转归及抗氧化体系的影响

Objective To observe the effects of indole-3-carbinol (I3C) on the outcome of the tumor as well as the changes of the anti-oxidative system in null mice grafted with nasopharyngeal carcinoma. Methods 48 BALB/c null mice were divided by means of random number table into control group (0. 5% sodium carboxyl methyl cellulose),low dosage (0.02 g/kg),middle dosage (0. 1 g/kg) and high dosage(0. 5 g/kg) of I3C. The mice were administered with different solutions by gavage for 10 days before CNEI cells were inoculated subcutaneously into the back (near the armpit) of the nude mice,then the solutions were continually administered by gavage. The tumor volume was measured and the tumor inhibitory rate was calculated. The level of malondialdehyde (MDA),the activity of superoxide dismutases (SOD),the activity of glutathione peroxidase (GSHPx) and the expression of cleaved caspase-3 were determined on the 31th day of the study. Results I3C could reduce the tumor volume [the tumor volumes of the control group,the middle dosage group and the high dosage group were(4. 13±0. 53)×10-6m3, (3.14±0.71)×10-6m3, (2. 72±0. 29)×10-6m3], as compared with the control, the shrinkage of tumor volume of the middle dosage group and the high dosage group were significant (the t valued at 0. 990 and 1.510, P< 0.01). The tumor inhibitory rates of 3 groups were 3.8%,20.5% and 34.9%,respectively. The contents of MDA in the tumor tissue tended to decrease [the values of control group,the low dosage group,the middle dosage group and the high dosage group were(31.29±2. 51)×10-6mol/L, (30. 12±2. 37)×10-6 mol/ L, (23. 32±1.93)×10-6 mol/L, (16.45±1.43)×10-6 mol/L](F=98. 752, P < 0. 01), and that of the high and the middle dosage group could obviously be reduced (t=8. 970,14. 840, P < 0.01) as compared with the control. The activity of SOD seemed to be elevated according to the increase of I3C dosage [the values were (387.24±23.16)×103 U/L, (399. 37±34. 45)×103 U/L, (431.63±31.24)×103 U/L, (476.45±44. 67)×103 U/L](F=53.444,P < 0. 01). When compared with the control, the SOD activity of the middle and the high dosage group be obviously increased (t=44. 390,89. 210,P <0.01). I3C could also elevate the GSHPx activity [the GSHPx values of the four groups were (226. 98±18.35)×103 U/L, (234.65±15.59)×103 U/L, (247.72±22. 73)×103 U/L, (300. 37±26. 02)×103 U/L](F=25.916,P < 0. 01). The GSHPx of the high dosage group was enhanced remarkable(t=73. 390, P < 0. 01) as compared with the control. The expression of cleaved caspase-3 (relative molecular weight=19 000 000) seemed to be elevated according to the increase of I3C dosage and the relative expression levels of wbich were 0. 87±0. 01,0. 97±0. 01,1.02±0. 06 and 1.14±0.02 (F=39. 864, P < 0. 01). When compared with the eontrol,the elevation of this kind of cleaved easpase-3 was considered statistical significant (the t values were 0. 100,0. 086 and 0. 303, respectively, P < 0. 05). When 13C dosage increased, the expression of cleaved caspase-3 (relative molecular weight=17 000 000) seemed to increased too [the relative expression levels of which were 0.00±0.00,0.05±0.02,0. 11±0.02,0.20±0.02(F=56.629,P<0.01)],and the increase of this kind of cleaved caspase-3 was esteemed significantly as compared with those of the control (the t valued at 0. 046,0. 103 and 0. 193, respectively, P < 0. 05). Linear correlate analysis showed that the correlation coefficients between the shrinkage of tumor volume and the expression of the two kinds of cleaved caspase-3 protein was-0.732 (t=3. 404, P < 0. 01) and-0. 901 (t=6. 642, P < 0. 01). Conclusion I3C could reduce the growth of tumor, the mechanism underlie it could be related to the decrease of the content of MDA as well as the elevated levels of SOD,GSHPx,and perhaps could be related to the apoptosis transducted by cleaved caspase-3.     

微生物蛋白质的毒性检测

微生物蛋白质是利用木薯渣经CO_(13)菌株发酵的产品,蛋白质含量30％,由广东省微生物研究所提供。它可广泛用作猪、鸡及其他动物饲料。我们对其作了毒性检测,结果如下:     

烹饪油烟对大鼠SOD、MDA及免疫器官影响的研究

目的 :研究烹饪油烟对大鼠血清 SOD (超氧化物歧化酶 )活性、MDA (丙二醛 )含量及免疫器官的影响。方法 :给大鼠分别经腹腔一次注入 80 0 0 mg/ kg、 40 0 0 mg/ kg、 2 0 0 0 mg/ kg的烹饪油烟凝集物 ,同时设对照组 ,6d后颈动脉取血并处死动物 ,测定血清中 SOD活性、 MDA含量 ,观察对免疫器官的影响。结果 :烹饪油烟可使大鼠血清中 SOD活性显著降低 ,MDA含量明显升高 ,与对照组比较 P<0 .0 5及 P<0 .0 1,并对胸腺和脾脏产生不同程度的病理损伤。结论 :烹饪油烟可破坏机体氧化与抗氧化的动态平衡 ,并损及机体免疫器官的结构和功能