过氧化苯甲酰对面粉中B族维生素含量影响的研究

为探讨面粉处理剂－－过氧化苯甲酰对面粉中维生素的影响,采用新加工的小麦标准粉,按0．00,0．03,0．06,0．10,0．20kg/kg剂量添加过氧化苯甲酰,库存条件下放置21天,利用高效液相色谱法分析面粉中维生素含量,结果表明,面粉中硫胺素,核黄素,烟酸均随着过氧化苯甲酰的剂量增加而减少,并呈剂量反应负相关关系,提示过氧化苯甲酰对面粉中硫胺素,核黄素和烟酸有明显的破坏作用。     

过氧化苯甲酰对面粉中B族维生素含量影响的研究

为探讨面粉处理剂——过氧化苯甲酰对面粉中维生素的影响,采用新加工的小麦标准粉,按0.00,0.03,0.06,0.10,0.20g/kg 剂量添加过氧化苯甲酰,库存条件下放置21d,利用高效液相色谱法分析面粉中维生素含量.结果表明,面粉中硫胺素、核黄素、烟酸均随着过氧化苯甲酰的剂量增加而减少,并呈剂量反应负相关关系.提示过氧化苯甲酰对面粉中硫胺素、核黄素和烟酸有明显的破坏作用.     

无患子皂苷对自发性高血压大鼠的血压调节机制研究

目的 探讨无患子皂苷对自发性高血压大鼠的血压调节机制.方法 40只自发性高血压大鼠随机分为模型组、阳性对照组、无患子皂苷低剂量组、无患子皂苷中剂量组、无患子皂苷高剂量组,每组8只.8只健康SD大鼠设为对照组.对照组、模型组大鼠每日灌胃生理盐水,10 mL/(kg·d),连续灌胃14 d.阳性对照组每日灌胃替米沙坦,20 mg/(kg·d),连续灌胃14 d.无患子皂苷低剂量组、中剂量组、高剂量组,分别每日灌胃无患子皂苷20 mg/(kg·d)、50 mg/(kg·d)、70 mg/(kg·d),连续灌胃14 d.各组大鼠分别于每日给药前、给药后1、2、3 h测定尾动脉收缩压.各组大鼠分别于末次给药后1 h,采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清中一氧化氮(nitric oxide,NO)、内皮素-1(endothelin-1,ET-1)、血栓素A2(thromboxane A2,TXA2)、白介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)、C反应蛋白(C-reactive protein,CRP)水平.结果 给药后,阳性对照组、无患子皂苷各组收缩压值均显著低于给药前和模型组(P<0.05).给药后,无患子皂苷各组的大鼠尾动脉收缩压值与阳性对照组间无显著差异(P>0.05).阳性对照组、无患子皂苷各组的血清NO水平显著高于模型组(P<0.05),血清ET-1、TXA2水平均显著低于模型组(P<0.05).无患子皂苷剂量越高,血清NO水平越高,血清ET-1、TXA2水平越低.结论 无患子皂苷对自发性高血压的降压作用明显,其作用机制可能与改善血管内皮功能、降低机体炎症水平有关.     

成人骶前囊肿再手术10例临床分析

目的探讨成人骶前囊肿再手术的临床特点和原因。方法对成人骶前囊肿再手术10例的临床资料进行回顾性分析。结果本组再手术距首次手术时间1个月～3年。再手术经骶尾部入路9例,经腹及骶尾部联合入路1例,均完整切除囊壁,顺利完成手术,术后伤口愈合时间平均38 d,术后平均随访6.5个月,均无复发。结论导致成人骶前囊肿再手术的主要原因为对此病认识不足,其次为术中囊壁切除不彻底、形成窦道经久不愈。加强对此病的认识,完善术前影像学检查,选用恰当手术方式,完整切除囊壁,是避免骶前囊肿再手术的关键。     

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我院 1987～ 2 0 0 0年间收治脾动脉瘤 12例。现报告如下。1　临床资料　　一般资料　本组 12例 ,男 7例 ,女 5例。年龄 14～ 6 8岁 ,平均 4 6岁。术前影像学检查发现瘤体 10例 ,另 2例术中探查发现。瘤体最小 2 0ｃｍ× 3 8ｃｍ ,最大 5 2ｃｍ×6 0ｃｍ。 7例有肝硬化门静脉高压病史 ,1例有动脉粥样硬化病史。　　临床表现　除 1例行健康体检时发现 ,无明显症状外 ,本组病例大多症状笃重 :急性腹痛 8例 ,瘤体破裂出血 2例 ,其中并消化道出血 2例 ,腹腔血肿 2例 ,扪及腹部包块伴腰痛腹痛 2例 ,脾肿大脾机能亢进 5例。　　影像学检查　本…     

无患子皂苷对脑缺血再灌注损伤模型大鼠炎症因子的影响

目的：探讨无患子皂苷对脑缺血再灌注损伤模型大鼠炎症反应及其作用机制。方法：选取80只雄性SD大鼠,随机均分为假手术组、模型组、阳性药物组、无患子大、小剂量组。采用线栓法复制大脑中动脉缺血再灌注模型,缺血2h,随即实施再灌注,再灌注时间为22h。取血后用ELLISA法检测各组动物血清白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α,取脑一部分采用TTC染色观察梗死面积,其他匀浆后测黏附分子-1的含量。结果：与模型组比较,药物组IL-1β、TNF-α、ICAM-1含量显著降低,IL-6含量上升,脑梗死率明显降低。结论：无患子皂苷可抑制脑缺血再灌注损伤过程中的炎症反应;其机制可能与抑制炎症因子的级联反应以及其在血液和脑组织内的含量有关。     

Expressions of connexin and par-3 in the distal margin of rectal cancer after ultra-low anterior resection

This study examined the expression of connexin and protease-activated receptor 3 （par-3） in the distal resection margin of rectal cancer and the correlation of the expression of the two proteins with tumor relapse. A total of 40 patients with rectal cancer underwent ultra-low anterior resection with curved cutter stapler. The pathological specimens were divided into 3 groups in terms of sampling sites： tumor group, 2.0-cm group （in which the tissues were harvested 2.0 cm distal to the tumor tissues）, 3.0-cm group （in which the tissues were taken 3.0 cm away from the tumor tissues）. All the samples were pathologically observed and then measured for the expression of connexin and par-3 by employing immunohistochemistry and Western blotting. The operations in this series went uneventfully. No anastomotic stoma bleeding, stenosis and death occurred postoperatively. Histopathologically, in the tumor group, epithelial cells lost normal pattern of arrangement and polarity, and were loosely connected and even detached. In the 3.0-cm group, the epithelia had normal appearance, obvious cell polarity and essentially intact cell junction. Immunohistochemistry and Western blotting indicated that the 3.0-cm group had the strongest expression of connexin and par-3, and the expression in the 2.0-cm group and the tumor group was relatively weak. There existed significant difference in the expression of the two proteins among the three groups （P〈0.05 for all）. It was concluded that the down-regulated connexin and par-3 in the distal margin of rectal cancer tissues may indicate the progression of the disease and high likelihood of recurrence and metastasis. Although no tumor cells were found in the sections of the 2.0cm group, the decreased expression of connexin and par-3 may suggest the development of anaplasia and the increased odds of tumor relapse. Therefore, we are led to speculate that tumor resection only including 2.0 cm of unaffected rectum could not completely avoid the distant metastasis and local relapse.     

胆囊平滑肌肉瘤一例

病人,女,74岁,间歇性右上腹疼痛3年余,与进食油腻食物有关,疼痛向腰、肩部放射,无黄疸,无恶心呕吐,无腹泻。经抗炎治疗疼痛可缓解,B 超提示“胆囊多发结石、胆囊炎”,入院体检:巩膜及皮肤无黄染,全身浅表淋巴结不肿大,右上腹压痛,无反跳痛,Murphy 征(-),无腹水征。实验室检查:血常规:Hb 9.3g/L,RBC 3.39×10~9/L,WBC 6.1×10~9/L,中性粒细胞: