铝尘和石英粉尘对肺泡巨噬细胞表面形态的影响

用体外培养和扫描电镜技术研究铝尘和石英尘对兔肺泡巨噬细胞表面形态学的影响。结果表明两种粉尘均引起巨噬细胞吞噬和激活,细胞激活表现为表面皱褶或指状突起增大增粗;随培养时间延长,细胞表面皱褶或突起减少,表面光滑,用台盼蓝染色测定膜透过性增加。石英尘对细胞损害作用强于铝尘,表现为失去巨噬细胞表面结构特征的细胞聚集成堆、细胞膜出现“洞眼”样缺损;而用铝尘处理的巨噬细胞表面出现泡状结构,这一形态学改变的意义有待深入研究。     

卫氏并殖吸虫毛蚴超微结构的观察

在SEM下,卫氏并殖吸虫(三倍体型)毛蚴体表密布细长的纤毛。TEM示纤毛的微管呈典型的“9+2”形式排列。除纤毛外,体表密布一层点线状胞质突起。体表下依次为基质、基膜和肌肉层。肌肉层下方见一含大量直径为0.15μm左右的圆形结构的囊细胞。焰细胞2个,大小为3×5.4μm,胞质内含有许多线粒体。焰细胞的纤毛束由39～45根纤毛组成。胚细胞在体后部集中成团,体前、中部亦有分布。     

改制JSM—T300部分装置及确立照像条件

JSM-T300型扫描电镜,是主机附设黄金溅射镀膜装置的简易扫描电镜,因售价低,国内已引进7台。该机的各项性指标都比较低。我们在提高照像质量的同时,吸收日立型扫描电镜标本装置的优     

脑室内猪囊虫病

脑室内猪囊虫病占脑囊虫病的17％。如手术及时,效果较好。作者根据我院近年收治的28例脑室内囊虫病,并对其中2例的囊虫囊胞以扫描电镜进行了观察,现就有关的问题讨论如下。 一般资料 本组病例均经手术和病理检查证实。28例中男16例,女13例。年龄11～63岁,其中11～20岁9例,24～30岁5例,31～40岁4例,41～50岁4例,51～60岁3例,61岁以上者3例。囊虫位于第四脑室内26例(93％),位于侧脑室内2例(7％)。     

大平并殖吸虫后尾蚴的扫描电镜观察

大平并殖后尾蚴在扫描电镜下体表及吸盘上密生单生棘。口吸盘及腹吸盘上各具有两圈感觉乳突。体表除散在有感觉乳突外,在腹面两外侧各具有一排对称性感觉乳突。     

颅内占位性病变的钙化及其临床意义的初步探讨

作者报道39例由颅内占位性病变而具有钙化斑的病人,根据CT所见,提出被此鉴别的要点。用腐烂和剥离法取出标本中的钙斑,以光镜和扫描电镜对其做了观察,见到随病变性质不同而钙斑的形状结构也不同。5例钙斑还以扫描电镜能谱仪进行了成份分析,结果是:所有钙化斑除含有钙磷外,还含有硅,而其他成份如钾、镁、铁、硫等则是可有可无。为何在病变处出现钙质增多?经讨论后认为,那可能是免疫或其他反应的伴随现象,有助于限制病变的发展或向良性方面转化。     

扫描电镜连续观察长发的方法及应用

应用扫描电镜观察毛发的外部形态,常因标本台小,而取毛发中的几段(每段约10mm),以导电胶分别将毛发的两端固定在标本台上,然后喷金观察。这种方法不能完整地观察一根长发。为了提高     

华支睾吸虫尾蚴扫电镜观察

The surface structure of Clonorchis sinensis cercariae was observed under scanning electron microscope. The cercaria was of the parapleuro-lophocercous type. The measurements of the cercariae were as follows: body 137-240 x 62-90 microns, tail 320-470 x 21-34 microns, oral sucker 27-46 x 23-33 microns. The ventral sucker was incompletely developed and much smaller than the oral one. The whole body surface was covered with backward pointing spines. Four horizontal rows of oral spines were found around the mouth opening of the oral sucker. These spines diminished in size with the distance of the rows from the opening and their numbers were 12, 18, 19 and 18 respectively. Transverse and longitudinal plicae formed from tegument were found on the surface of the tail, being in connection with dorsal and ventral fins along posterior half of the tail. Many sensory structures or papillae were observed on the body. They were of three different types: 1) papillae with long or short cilia distributed widely on the body surface and around the mouth opening, the number of papillae with cilia on the body surface being greater than that described by Komiya et al (1940); 2) a few sensory fossa on the tail, measuring 1.0 x 0.6 microns; 3) a few ring-type papillae with knob on the body surface with pore of 0.5 micron in diameter.     

手鱼际部皮肤乳头层血管形态的扫描电镜观察

本文用二侧成人新鲜上肢,经肱动脉灌注ABS丁酮溶液,喷金后,用扫描电镜观察了手鱼际部皮肤乳头层微细血管的形态特点,将其分为五型:(1)发卡型;(2)球团型;(3)螺旋型;(4)双袢型;(5)不规则型。对各型的特点进行了描述。     

家兔视网膜扫描电镜断层图象观察

视网膜是视觉感受部位,由10层所构成。应用光镜及电镜观察视网膜,对其构造成超微结构已有较详细的了解。我们对家兔视网膜的断裂扫描电镜观察时,得出层次清晰,立体感强的图象,可以做为光镜和透射电镜的补充,有助于对视网膜构造的认识,现简解如下: 标本制作方法:正常家兔在乙醚麻醉下摘除眼球,剖开眼球清除玻璃体,用1%α—糜蛋白酶及生理盐水冲洗视网膜表面后,用虹膜恢复器轻轻地